Background: Some researchers attributed the positive effects of exercise training on secretion of different myokines from skeletal muscles. Acute exercise lead to changes in gene expression and phosphorylation that stimulates muscular adaptation. However, one bout exercise isn’t adequate to change muscle phenotype and phenotypic adaptation to training consists of aggregation stimulation of one bout exercise sessions Decorin is new discovered myokine that its changes in response to exercise such as high intensity interval exercise (HIIE) is unknown. It seems that Decorin has effects on skeletal muscle hypertrophy. Therefore, the aim of present study was investigated the effect of one bout high intensity interval exercise (HIIE) on serum levels of decorin and IGF-I in active young male. Materials and Methods: For this purpose, 10 active young males with mean age of 25.4±2.36 voluntary participated in this study. The subjects took part in HIIE protocol in 10 am and after 3 hours. HIIE protocol consisted of four minutes' intervals with 90-95 percent of maximum heart rate that between each interval, 3-minute active recovery with 60-70 percent of maximum heart rate performed. The subjects heart rate during HIIE protocol was monitored by polar belt. Blood samples were collected immediately after exercise, subsequently IGF-I and decorin levels were measured by ELISA method. In order to data analyzed, SPSS software version 24 and paired t test were used and significantly level was considered p<0.05. Results: The results indicated that decorin and IGF-I increased significantly after exercise (p≤0/05). Conclusion: It seems that some HIIT-induced adaptations partly are related to increase in decorin levels. Manuscript profile

Background: Deep-fried oils (DFO) produce toxins that endanger people's health. Using herbal supplements along with exercise training can help improve health. The purpose of this study was to investigate the effect of aerobic training (T) and octopamine (O) supplementation on VEGF and PDGFR gene expression levels in the heart tissue of rats poisoned with DFO. Materials and Methods: In this experimental study, 25 male Wistar rats were purchased and placed in 5 groups of 5 animals, including: 1) control, 2) DFO, 3) DFO+T, 4) DFO+O and 5) DFO+T+O. Over a course of 4 weeks, rats in groups 2 to 5 received DFO by gavage, and rats in groups 3 and 5 ran on the treadmill 5 times a week with moderate intensity; also, rats in groups 4 and 5 received 81 μmol/kg octopamine supplement intraperitoneally 5 days a week. The Kolmogorov-Smirnov statistical test, independent samples t-test and two-way analysis of variance were used to analyze the research findings (p<0.05). Results: DFO had a significant effect on increasing the gene expression levels of VEGF (p=0.001) and PDGFR (p=0.002); training had a significant effect on the reduction of VEGF (p=0.001) and PDGFR (p=0.012); also, octopamine consumption had a significant effect on the reduction of VEGF (p=0.001) and PDGFR (p=0.045). Conclusion: It seems that aerobic training and octopamine consumption alone have anti-inflammatory effects in the heart tissue of DFO-poisoned rats, however they do not have significant anti-inflammatory interactive effects. Manuscript profile

Background: Osteoarthritis (arthritis of the joints) is one of the most common metabolic disorders of bone tissue that reduces the process of absorption and reabsorption in bone. Exercise and stem cell injections can have beneficial effects in treating this disease. The enzymes alkaline phosphatase and osteopontin, as markers of bone formation, play an important role in diagnosing the progression or treatment of this disease. The aim of this study was to examine the effect of training, stem cells and hyaluronic acid on osteocalcin, ALP and osteopontin in the cartilage tissue of rats with osteoarthritis. Materials and Methods: In this study, 25 rats were divided in 5 groups including: (1) healthy control, (2) patient control, (3) endurance training (3 days a week for one month), (4) recipients of mesenchymal stem cells (1 × 106 cells / Kg), and (5) simultaneous recipients of endurance training and mesenchymal stem cells. Alkaline phosphatase gene expression was assessed by RT PCR and the amount of osteopontin synthesis was measured by immunohistochemistry procedure. Results: Training and mesenchymal stem cell injection had a significant effect on increasing alkaline phosphatase gene expression and osteopontin in patient rats compared to the patient control group (P <0.001). Also, simultaneous endurance training and stem cell injection have interactive effects on increasing both factors (P <0.001). Conclusion: Based on the findings of this study, it seems that endurance training and injection of mesenchymal stem cells in the joints, either separately or simultaneously, can increase the expression of alkaline phosphatase gene and the amount of osteopontin. Manuscript profile

Background and purpose: the use of heated oils has become an integral part of today's nutrition. Studies show that with the development of obesity, capillarization in adipose tissue does not occur in line with changes in cell size. Therefore, adipose tissue in obese people is always associated with lack of oxygen and it causes systemic inflammation by releasing inflammatory mediators. The purpose of this study is to investigate the effect of aerobic exercise and octopamine on HIF-1 protein concentration in visceral fat and white cells in rats fed with deeply-heated oil. Methodology: In an experimental trial, 30 male Wistar rats were divided into five groups: healthy control, control-heated oil, aerobic exercise-heated oil, octopamine-heated oil and exercise, and octopamine-heated oil. Octopamine was given to rats by IP intraperitoneal injection daily for four weeks and five days a week. Aerobic exercise was also performed for four weeks and five days a week with moderate intensity on the treadmill. Forty-eight hours after the last intervention, the rats were anesthetized and visceral adipose tissue was removed from the body to measure HIF-1a gene expression. As a result of receiving deeply-heated oil, the expression of HIF-1 gene and protein in visceral fat increased significantly (P=0.001), but the number of white cells in visceral adipose tissue increased significantly (P=0.001). Aerobic exercise significantly decreased HIF-1 gene and protein expression (P=0.01). In addition, octopamine supplementation had no significant effect on HIF-1 gene expression of visceral fat of white cells in rats poisoned with deeply-heated oil. Receiving octopamine also decreased HIF-1 gene and protein expression (P=0.002). In addition, exercise significantly reduced the number of white cells (P=0.001). Octopamine could significantly reduce the expression of HIF-1 protein and the number of white cells. The interaction of exercise and octopamine was significant for the expression of HIF-1 protein and the number of white cells. Findings: The results of this study showed that aerobic exercise and octopamine improve the angiogenesis process of the visceral adipose tissue that had been disrupted by heated oils, and reduce the damage caused by feeding with deeply-heated oils. Manuscript profile

Background: Resistance training improves skeletal muscle function by affecting the proteins of the nervous system. However, there are conflicting results regarding the effects of resistance training on Neural cell adhesion molecule (NCAM) gene expression. Therefore, the present study aimed to investigate the effect of 4 weeks of increasing resistance training on NCAM gene expression in the gastrocnemius muscle of healthy male rats. Materials and Methods: In an experimental trial, 12 young male rats were randomly divided into 2 groups of 6, including the control and resistance training groups. The training group performed increasing resistance training 5 days a week for 4 weeks on a special rodent ladder. Forty-eight hours after the end of the training intervention, the rats were sacrificed and the gastrocnemius muscle tissue was extracted for the expression of the NCAM gene using the real-time method. Results: resistance training in the neuromuscular junction, gastrocnemius muscle increased NCAM gene expression (P=0.036) compared to the control group. Conclusion: Four weeks of resistance training can improve skeletal muscle function by increasing NCAM gene expression at the end of muscle fibers. Manuscript profile