Background and purpose: the use of heated oils has become an integral part of today's nutrition. Studies show that with the development of obesity, capillarization in adipose tissue does not occur in line with changes in cell size. Therefore, adipose tissue in obese people is always associated with lack of oxygen and it causes systemic inflammation by releasing inflammatory mediators. The purpose of this study is to investigate the effect of aerobic exercise and octopamine on HIF-1 protein concentration in visceral fat and white cells in rats fed with deeply-heated oil. Methodology: In an experimental trial, 30 male Wistar rats were divided into five groups: healthy control, control-heated oil, aerobic exercise-heated oil, octopamine-heated oil and exercise, and octopamine-heated oil. Octopamine was given to rats by IP intraperitoneal injection daily for four weeks and five days a week. Aerobic exercise was also performed for four weeks and five days a week with moderate intensity on the treadmill. Forty-eight hours after the last intervention, the rats were anesthetized and visceral adipose tissue was removed from the body to measure HIF-1a gene expression. As a result of receiving deeply-heated oil, the expression of HIF-1 gene and protein in visceral fat increased significantly (P=0.001), but the number of white cells in visceral adipose tissue increased significantly (P=0.001). Aerobic exercise significantly decreased HIF-1 gene and protein expression (P=0.01). In addition, octopamine supplementation had no significant effect on HIF-1 gene expression of visceral fat of white cells in rats poisoned with deeply-heated oil. Receiving octopamine also decreased HIF-1 gene and protein expression (P=0.002). In addition, exercise significantly reduced the number of white cells (P=0.001). Octopamine could significantly reduce the expression of HIF-1 protein and the number of white cells. The interaction of exercise and octopamine was significant for the expression of HIF-1 protein and the number of white cells. Findings: The results of this study showed that aerobic exercise and octopamine improve the angiogenesis process of the visceral adipose tissue that had been disrupted by heated oils, and reduce the damage caused by feeding with deeply-heated oils. Manuscript profile