List of Articles Enzyme

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  1 - Evaluation and comparison of some green methods for synthesis of silver nanoparticles
  sepide hamedi seyed abbas  shojaossadati
  10.29252/.3.5.59
  Nowadays, there is an increasing need to develop high-yield, low cost, nontoxic, and eco-friendly procedures for synthesis of metallic nanoparticles. Therefore, the green synthesis methods become customary for synthesis of nanoparticles. Among metallic nanoparticles, na More

  Nowadays, there is an increasing need to develop high-yield, low cost, nontoxic, and eco-friendly procedures for synthesis of metallic nanoparticles. Therefore, the green synthesis methods become customary for synthesis of nanoparticles. Among metallic nanoparticles, nanosilver has developed because of its therapeutic properties. So in this paper, polysaccharide, tollens and biological green methods were investigated. In polysaccharide and tollens methods, starch and β-D glucose were used as a satabilizer and reducer respectively. In biological method biomass and cell filtrate of the Fusarium oxysporum fungus were used for the synthesis of nanoparticles. SEM images and UV-visible absorbtion spectra of these procedures showed that the polysaccharide method produced smaller silver nanoparticles wih high productivity. The changes of NADH-dependant nitrate reductase enzyme activity was evaluated in growh duration by colorometric Harely method due to importance of this enzyme in extracellular synthesis of silver nanoparticles. Results showed that the changes of dry cell mass impact on the enzyme activity. Manuscript profile
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  2 - Laboratory diagnosis of liver disease in small animal practice
  Saba Ahmadi Morteza  Hasanabadi Mehrdad Mohri
  Today’s accurate diagnosis of disease requires using of different diagnostic and paraclinical methods. Diagnosis of liver disease was a serious challenge both in medicine and veterinary medicine from the past. Clinical biochemistry is one of the main parts of diagnostic More

  Today’s accurate diagnosis of disease requires using of different diagnostic and paraclinical methods. Diagnosis of liver disease was a serious challenge both in medicine and veterinary medicine from the past. Clinical biochemistry is one of the main parts of diagnostic methods. Liver function is evaluated by measuring the variables such as excreting and metabolic functions and enzymes. Because of large functional reserve of liver, symptoms of liver disease appear after loss of huge number of hepatocytes, therefore using of laboratory methods with high specificity and sensitivity could be helpful. None of existing laboratory methods has all characteristics mentioned above. It seems that using different laboratory methods of liver function beside other diagnostic methods such as sonography, cytology and … could be an appropriate approach for reaching a diagnosis of hepatobiliary disease. Current article reviews the perfect utility of liver function tests for general diagnosis of liver disease. Manuscript profile
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  3 - The Effects of Coenzyme Q10 Supplementation with Two Consecutive Soccer Games on Stress Oxidative and Muscle Injury Markers in Male Collegiate Soccer Players
  Ehsan Arabzadeh
  10.61186/jspac.19775.1.1.1
  Background: This study aimed to investigate the effect of coenzyme Q10 consumption for fourteen days on the response of some indicators of oxidative stress and muscle damage following two consecutive football matches in college players Materials and Methods: for this p More

  Background: This study aimed to investigate the effect of coenzyme Q10 consumption for fourteen days on the response of some indicators of oxidative stress and muscle damage following two consecutive football matches in college players Materials and Methods: for this purpose, 24 football players (with an average age of 20.08±12.1 years, a weight of 63.28±65.1 kg and a maximum oxygen consumption of 53.12± 0.98 ml /per /kg of body weight) The three groups were studied over a fourteen-day period. The control group did not receive these supplements and exercise activities, but the experimental groups included the placebo group (300 mg aspartame) and the coenzyme Q10 group (300 mg ubiquinone supplement) during the course in addition to receiving a placebo or supplement the two 90-minute football matches were less than 48 hours apart. Then, according to the research plan, blood samples were collected from the subjects in two stages 24 hours before and after the period and MDA, LDH, CK and AST indices were measured. Dependent t-test, one-way analysis of variance and Tukey's post hoc test were also used. Results: The results showed a significant difference between serum coenzyme Q 10 supplementation with serum MDA (P = 0.000) and AST (P = 0.006) concentrations from two consecutive football matches, while LDH levels (P = 0.970) and serum CK (P = 0.911) did not change significantly. Conclusion: Overall, the results show that supplementing coenzyme Q10 before and during a two-week match can have anti-oxidant benefits, so it can be recommended to college soccer players. Manuscript profile
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  4 - Comparing the effect of antioxidant and coenzyme 10Q supplementation on some indicators of muscle injury in water polo boys
  Peyman Azarmidakhtian Alireza  Eizadi Mohammad Samadi
  10.61186/jspac.33237.2.3.5
  Background: Reactive oxygen species are produced in response to strenuous, prolonged exercise, such as swimming, which results in cell damage. The aim of this study was to compare the effect of antioxidant and coenzyme 10Q supplementation on some indicators of muscle in More

  Background: Reactive oxygen species are produced in response to strenuous, prolonged exercise, such as swimming, which results in cell damage. The aim of this study was to compare the effect of antioxidant and coenzyme 10Q supplementation on some indicators of muscle injury in water polo boys. Materials and Methods: The 24 boy water polo players with at least 5 years of training experience and in the age range of 17 to 23 years were randomly divided into three groups )8 people (of vitamin C, coenzyme Q10 and control. Subjects in the vitamin C group (500 mg of vitamin C tablets) and subjects in the coenzyme Q10 group (300 mg of coenzyme Q10 tablets) were consumed daily with food for two weeks. Trainings were performed for two weeks, 6 sessions per week and 90 minutes per session. Evaluation of CK, LDH and AST indices in the state of at least twelve hours of fasting was performed in three stages: previous, immediately and 24 hours after the completion of the protocol. One-factor analysis of variance test with repeated measures was used. Results: The results showed that CPK decreased significantly after fourteen days of supplementation in coenzyme Q10 group and increased significantly 24 hours after the last training session and in vitamin C group after fourteen days of supplementation and 24 hours after the last training session increased significantly. There was no significant difference in LDH and AST levels between the groups (P <0.05). Conclusion: It seems taking coenzyme Q10 supplements may possibly reduce some indicators of muscle damage after water polo training. Manuscript profile
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  5 - The efficiency of crude enzyme extracted from horseradish root in oxidation of catechol
  Soheila Amiri Hosseini Fatemeh Nejatzadeh Eesmaeil  Babanezhad fathollah gholami
  Catechol is one of the phenolic compounds (with chemical formula (C6H6O2) which is used as a raw material or final product of chemical, petrochemical and oil refineries. In this study, the efficiency of horseradish extract as a gross enzyme and removal of catechol from More

  Catechol is one of the phenolic compounds (with chemical formula (C6H6O2) which is used as a raw material or final product of chemical, petrochemical and oil refineries. In this study, the efficiency of horseradish extract as a gross enzyme and removal of catechol from industrial wastewater was investigated. In order to study the efficiency of variable enzyme processes (20, 10, 40, ml / l), oxygenated water concentrations (0.58, 1.17, 2.35 mol / l) were evaluated for 20 minutes. The output catechol concentration of the process was measured by HPLC at a wavelength of 275 nm. The results of this study showed that with increasing the volume of oxygenated water and keeping the concentration of the enzyme constant, an increasing trend was observed at first, but after a while with a further increase in hydrogen peroxide, no significant change in efficiency was observed. For concentrations of hydrogen peroxide (0.58, 1.17, 1.78 mol / L) and hydrogen peroxide (10, 20, 40 ml / L), removal efficiencies of 45%, 60% and 80% were obtained, respectively. However, with the values of oxygenated water concentrations (1.17, 2.35, 3.53 mol / l) and fixed oxygenated water concentrations, the removal efficiency was 42%, 58% and 81%, respectively. Enzymatic treatment process as a suitable process can be used for catechol and its compounds from wastewater. To obtain the appropriate efficiency, it is better to set it in the optimal conditions under optimal conditions and add variables in the reaction. Manuscript profile
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  6 - Isolation and screening of pectinase producing Aspergillus niger and use of whey to optimize enzyme production
  Mahsa pouya shokoofeh Ghazi Amir Tukmechi
  Aims and Background: Pectinase that can break down pectin in plant cell walls is one of the most important industrial enzymes in the world, which can be isolated from a wide range of microorganisms such as bacteria and fungi. Therefore, considering the importance and ap More

  Aims and Background: Pectinase that can break down pectin in plant cell walls is one of the most important industrial enzymes in the world, which can be isolated from a wide range of microorganisms such as bacteria and fungi. Therefore, considering the importance and applications of this enzyme in food processing, agriculture, the purpose of this research is isolating and screening pectinase-producing fungi from some of rotten fruits and vegetables, as well as optimizing the enzyme production condition. Materials and methods: Isolation and identification of pectinase producing fungi from rotten fruits (peach, plum and apple) and vegetables (onion) were done. Isolation and screening the most capable fungal strains producing pectinase was used by cultivation on specific Pectin Agar medium, staining with lactophenol cotton blue and slide culture method. In order to identify the isolated fungi more accurately, the molecular technique like 18S rRNA sequencing was used. Optimizing the production rate of pectinase enzyme carried out using pectin substrate accompanied by different Lactic Whey and Permeate whey under different temperatures and pH levels, and the activity of enzyme was measured by the standard DNS method. Results: one Aspergillus niger fungus producing pectinase enzyme was isolated from onion. Results for optimization conditions shows that the identified strain, shows an enzyme activity equal to 103/018 (IU/ml) in the presence of permeate whey and pectin substrate, under incubation at 25 C° ,140 rpm, and pH 6.5. While, pectinase production with only pure pectin substrate was lower equal to 92.717 (IU/ml) when incubation at 25 C° , same rpm and pH 7. Conclusions: Potent native fungal strains isolated from our country are suitable candidates for the production of important industrial enzymes such as pectinase. Also using cost effective substrates like Whey can be replaced by expensive pure substrates in production industry for the important enzymes production process. Manuscript profile