The efficiency of crude enzyme extracted from horseradish root in oxidation of catechol
Subject Areas : Water and wastewater technologySoheila Amiri Hosseini 1 , Fatemeh Nejatzadeh 2 , Eesmaeil Babanezhad 3 , fathollah gholami 4
1 - MSc student in Environmental Health Engineering, Faculty of Health, Mazandran university Sciences, Sari, Iran
2 - Department of Horticulture, Faculty of Agriculture, Khoy Branch, Islamic Azad University, Khoy, Iran
3 - Department of Environmental Health Engineering, Faculty of Health, Mazandran University of Medical Sciences, Sari, Iran
4 -
Keywords: Enzyme, Horse radish peroxidase, Catechol, Synthetic wastewater,
Abstract :
Catechol is one of the phenolic compounds (with chemical formula (C6H6O2) which is used as a raw material or final product of chemical, petrochemical and oil refineries. In this study, the efficiency of horseradish extract as a gross enzyme and removal of catechol from industrial wastewater was investigated. In order to study the efficiency of variable enzyme processes (20, 10, 40, ml / l), oxygenated water concentrations (0.58, 1.17, 2.35 mol / l) were evaluated for 20 minutes. The output catechol concentration of the process was measured by HPLC at a wavelength of 275 nm. The results of this study showed that with increasing the volume of oxygenated water and keeping the concentration of the enzyme constant, an increasing trend was observed at first, but after a while with a further increase in hydrogen peroxide, no significant change in efficiency was observed. For concentrations of hydrogen peroxide (0.58, 1.17, 1.78 mol / L) and hydrogen peroxide (10, 20, 40 ml / L), removal efficiencies of 45%, 60% and 80% were obtained, respectively. However, with the values of oxygenated water concentrations (1.17, 2.35, 3.53 mol / l) and fixed oxygenated water concentrations, the removal efficiency was 42%, 58% and 81%, respectively. Enzymatic treatment process as a suitable process can be used for catechol and its compounds from wastewater. To obtain the appropriate efficiency, it is better to set it in the optimal conditions under optimal conditions and add variables in the reaction.
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