Background: Fatty liver disease is the most important reason for chronic liver diseases such as liver fibrosis, cirrhosis and liver cancer. The aim of this study was the effect of endurance training and probiotic consumption on IL-18 gene expression in male rats with steatosis. Materials and Methods: In this experimental study, 32 rats (average weight 200-250 grams) were randomly divided into four groups (healthy control, modeled (steatosis) group, steatosis + probiotic group, and steatosis + probiotic + exercise group). Training groups for 8 weeks, 5 sessions every week, in the beginning week with a speed of 18 meters per minute, time of 10 minutes, and every week the speed of 1-2 meters per minute and the time was increased by 10 minutes, so that In the fourth week, the speed reached 22 meters per minute and the time reached 40 minutes. The groups receiving the supplement received 109 CFU/ml of Lactobacillus rhamnus GG by gavage daily for 8 weeks and 5 days a week. IL-18 gene expression in liver tissue was measured by Real-time PCR method. The data were evaluated using the statistical method of one-way analysis of variance and significance level (P ≥ 0.05). Results: The results showed that endurance training along with probiotic consumption significantly decreased IL-18 gene expression in rats with steatosis. Conclusion: It seems that performing endurance training along with probiotic consumption can have a greater effect than the effect of probiotic alone on IL-18 gene expression. Manuscript profile