The effect of endurance training and mesenchymal stem cells on ALP gene expression and osteopontin levels in rats with knee osteoarthritis
Subject Areas : Sport Physiology
Marjan Haghjoo
1
(Department of Exercise Physiology, Islamic Azad University, Central Tehran Branch, Tehran,Iran.)
Mohammad Ali Azarbayjani
2
( Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran )
Seyed Ali Hosseini
3
(Department of Exercise Physiology, Islamic Azad University, Marvdasht Branch, Marvdasht,Iran.)
Maghsoud Peeri
4
(Department of Exercise Physiology, Islamic Azad University, Central Tehran Branch, Tehran,Iran.)
Keywords: Osteoarthritis, Mesenchymal stem cells, Endurance training, Osteopontin, Alkaline phosphatase.,
Abstract :
Background: Osteoarthritis (arthritis of the joints) is one of the most common metabolic disorders of bone tissue that reduces the process of absorption and reabsorption in bone. Exercise and stem cell injections can have beneficial effects in treating this disease. The enzymes alkaline phosphatase and osteopontin, as markers of bone formation, play an important role in diagnosing the progression or treatment of this disease. The aim of this study was to examine the effect of training, stem cells and hyaluronic acid on osteocalcin, ALP and osteopontin in the cartilage tissue of rats with osteoarthritis. Materials and Methods: In this study, 25 rats were divided in 5 groups including: (1) healthy control, (2) patient control, (3) endurance training (3 days a week for one month), (4) recipients of mesenchymal stem cells (1 × 106 cells / Kg), and (5) simultaneous recipients of endurance training and mesenchymal stem cells. Alkaline phosphatase gene expression was assessed by RT PCR and the amount of osteopontin synthesis was measured by immunohistochemistry procedure. Results: Training and mesenchymal stem cell injection had a significant effect on increasing alkaline phosphatase gene expression and osteopontin in patient rats compared to the patient control group (P <0.001). Also, simultaneous endurance training and stem cell injection have interactive effects on increasing both factors (P <0.001). Conclusion: Based on the findings of this study, it seems that endurance training and injection of mesenchymal stem cells in the joints, either separately or simultaneously, can increase the expression of alkaline phosphatase gene and the amount of osteopontin.
1. Silva ALP, Imoto DM, Croci AT. Comparison of crytoherapy, exercise and short waves in knee osteoarthritis treatment. Acta ortopedica brasileira. 2007 Mar 15(4):204-9. doi: 10.1590/S1413-78522007000400006
2. Shojaedin SS, Sahebozamani M, Mehrabian H. Knee Joint Osteoarthritis in Retired Professional Athletes and Non- athletic Persons. J Rafsanjan Univ Med Scie. 2012 Jul 11(3):247-58. URL: http://journal.rums.ac.ir/article-1-1432-en.html.
3. Bavardi Moghadam E, Shojaedin SS. The effect of eight weeks Aerobic training on functional indicators and range of motion in active older men with knee osteoarthritis. RJMS. 2017 Jun 24 (156):100-110. URL: http://rjms.iums.ac.ir/article-1-4567-en.html.
4. Ramezani M, Alizadeh MH, Kordi MR. Effect of intensity and volume of endurance training on the incidence of knee osteoarthritis in healthy male rats. Razi Journal of Medical Sciences. 2015 May 22(131):97-105. URL: http://rjms.iums.ac.ir/article-1-3789-en.html.
5. Mokbel AN, El Tookhy OS, Shamaa AA, Rashed LA, Sabry D, El Sayed AM. Homing and reparative effect of intra-articular injection of autologus mesenchymal stem cells in osteoarthritic animal model. BMC Musculoskelet Disord. 2011 Nov 15; 12:259. doi: 10.1186/1471-2474-12-259. PMID: 22085445; PMCID: PMC3232438.
6. Banfi G, Colombini A, Lombardi G, Lubkowska A. Metabolic markers in sports medicine. Adv Clin Chem. 2012;56:1-54. doi: 10.1016/b978-0-12-394317-0.00015-7. PMID: 22397027.
7. Bijeh, N. Moazami, M. Mansouri, J. Nematpour, F. Ejtehadi, MM. Effect of aerobic exercises on markers of bone metabolism in middle-aged women. Trauma Monthly. 2011 Jun 16:129-135. [Persian]
8. Killian G. Physiology and endocrinology symposium: evidence that oviduct secretions influence sperm function: a retrospective view for livestock. J Anim Sci. 2011 May 89(5):1315-22. doi: 10.2527/jas.2010-3349. Epub 2010 Oct 8. PMID: 20935135.
9. Alghadir AH, Aly FA, Gabr SA. Effect of Moderate Aerobic Training on Bone Metabolism Indices among Adult Humans. Pak J Med Sci. 2014 Jul 30(4):840-4. doi: 10.12669/pjms.304.4624. PMID: 25097528; PMCID: PMC4121709.
10. Riyahi Malayeri, S., Saei, M. Changes in Insulin resistance and serum levels of resistin after 10 weeks high intensity interval training in overweight and obese men. Sport Physiology & Management Investigations, 2019;10(4): 31-42.http://www.sportrc.ir/article_82662.html?lang=en.
11. Zilaeibouri SH, Peeri M. The effect of exercise intensity on the response of some of adipocytokines and biochemical marker of bone in obese and overweight young female. Iranian journal of endocrinology and metabolism. 2015; 16(6):425-432. URL: http://ijem.sbmu.ac.ir/article-1-1780-en.html. [Persian]
12. shariatzadeh, M., Moghadam, Z., Maleki, L., Keshavarz, E., Hedayati, M. Short-Term Effect of Two Types of High-Intensity Interval Training on Plasma Level of TNF-α, IL-6, CRP and Lipid Profile of Overweight Women. Journal of Sport Biosciences, 2017 Sep 9(2):195-207. doi: 10.22059/jsb.2017.128575.963.
13. Zhang H, Ji L, Yang Y, Wei Y, Zhang X, Gang Y, Lu J, Bai L. The Therapeutic Effects of Treadmill Exercise on Osteoarthritis in Rats by Inhibiting the HDAC3/NF-KappaB Pathway in vivo and in vitro. Front Physiol. 2019 Aug 20;10:1060. doi: 10.3389/fphys.2019.01060. PMID: 31481898; PMCID: PMC6710443.
14. Blaney Davidson EN, van Caam AP, van der Kraan PM. Osteoarthritis year in review 2016: biology. Osteoarthritis Cartilage. 2017 Feb 25(2):175-180. doi: 10.1016/j.joca.2016.09.024. Epub 2017 Jan 16. PMID: 28100421.
15. Riyahi Malayeri S, Kaka Abdullah Shirazi S, Behdari R, mousavi Sadati K. Effect of 8-week Swimming training and garlic intake on serum ICAM and VCAM adhesion molecules in male obese rats. . JSSU 2019; 26 (10) :867-878.URL: http://jssu.ssu.ac.ir/article-1-4695-en.html
16. Ghasemalipour H, Eizadi M. The Effect of Aerobic Training on Some Bone Formation Markers (Osteocalcin, Alkaline Phosphatase) in Asthma Treated with Inhaled Corticosteroids. Zahedan J Res Med Sci. 2018 Jan 20(1):56-63. doi:10.5812/zjrms.58477.
17. Tong X, Chen X, Zhang S, Huang M, Shen X, Xu J, Zou J. The Effect of Exercise on the Prevention of Osteoporosis and Bone Angiogenesis. Biomed Res Int. 2019 Apr 18;2019:8171897. doi: 10.1155/2019/8171897. PMID: 31139653; PMCID: PMC6500645.
18. Yang DH, Yang MY. The Role of Macrophage in the Pathogenesis of Osteoporosis. Int J Mol Sci. 2019 Apr 28;20(9):2093. doi: 10.3390/ijms20092093. PMID: 31035384; PMCID: PMC6539137.
19. Mancuso P, Raman S, Glynn A, Barry F, Murphy JM. Mesenchymal Stem Cell Therapy for Osteoarthritis: The Critical Role of the Cell Secretome. Front Bioeng Biotechnol. 2019 Jan 29;7:9. doi: 10.3389/fbioe.2019.00009. PMID: 30761298; PMCID: PMC6361779.
20. Hanna H, Mir LM, Andre FM. In vitro osteoblastic differentiation of mesenchymal stem cells generates cell layers with distinct properties. Stem Cell Res Ther. 2018 Jul 27;9(1):203. doi: 10.1186/s13287-018-0942-x. PMID: 30053888; PMCID: PMC6063016.
21. Roy K, Kanwar RK, Kanwar JR. Molecular targets in arthritis and recent trends in nanotherapy. Int J Nanomedicine. 2015 Aug 26;10:5407-20. doi: 10.2147/IJN.S89156. PMID: 26345140; PMCID: PMC4554438.
