The effect of aerobic training and curcumin supplementation on the expression of IGF-1 gene in muscle rat
محورهای موضوعی : تغذیه ورزشی
Masoumeh Hosseini
1
,
Hamid Vakilian
2
1 - Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.
2 - Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.
کلید واژه: IGF-1R, IGF-1, curcumin,
چکیده مقاله :
Background: The purpose of this research was to investigate the effect of four weeks of moderate aerobic training and nanocurcumin supplementation on IGF-1 gene expression and its receptor in the soleus muscle of rats. Materials and Methods: 32 male Wistar rats, approximately eight weeks old, with a weight range of 200±10 grams, were prepared and kept for one week under standard conditions of light, temperature, and humidity. Rats were randomly divided into 4 groups of 8 series: healthy control group, aerobic training group, nanocurcumin group and aerobic training + nanocurcumin group. The training protocol was for 4 weeks, 5 days a week, in the first week, 20 minutes of activity with a speed of 18 meters per minute, and finally in the 4th week, it reached 35 minutes of activity with a speed of 20 meters per minute. Nanocurcumin supplement was also used for each animal at 80 mg / kilogram of body weight. One-way analysis of variance and Bonferroni's post hoc test was used at the significance level (P<0.05). Results: The results showed an increase in IGF-1 and IGF-1R gene expression levels in the soleus muscle in all groups compared to the control group. Conclusion: The combination of training and nanocurcumin supplementation probably increases the antioxidant capacity of the body and causes a greater increase in the expression of the IGF-1 gene and IGF-1R in skeletal muscles, which indicates more anabolic effects.
Background: The purpose of this research was to investigate the effect of four weeks of moderate aerobic training and nanocurcumin supplementation on IGF-1 gene expression and its receptor in the soleus muscle of rats. Materials and Methods: 32 male Wistar rats, approximately eight weeks old, with a weight range of 200±10 grams, were prepared and kept for one week under standard conditions of light, temperature, and humidity. Rats were randomly divided into 4 groups of 8 series: healthy control group, aerobic training group, nanocurcumin group and aerobic training + nanocurcumin group. The training protocol was for 4 weeks, 5 days a week, in the first week, 20 minutes of activity with a speed of 18 meters per minute, and finally in the 4th week, it reached 35 minutes of activity with a speed of 20 meters per minute. Nanocurcumin supplement was also used for each animal at 80 mg / kilogram of body weight. One-way analysis of variance and Bonferroni's post hoc test was used at the significance level (P<0.05). Results: The results showed an increase in IGF-1 and IGF-1R gene expression levels in the soleus muscle in all groups compared to the control group. Conclusion: The combination of training and nanocurcumin supplementation probably increases the antioxidant capacity of the body and causes a greater increase in the expression of the IGF-1 gene and IGF-1R in skeletal muscles, which indicates more anabolic effects.
1. Stein, Angelica Miki et al. Physical exercise, IGF-1 and cognition A systematic review of experimental studies in the elderly. Dementia & Neuropsychologia [online]. 2018, v. 12, n. 2 [Accessed 19 December 2022], pp. 114-122. Available from:
2. Majorczyk M, Smoląg D. Effect of physical activity on IGF-1 and IGFBP levels in the context of civilization diseases prevention. Rocz Panstw Zakl Hig. 2016;67(2):105-11. PMID: 27289505.
3. Nindl BC, Santtila M, Vaara J, Hakkinen K, Kyrolainen H. Circulating IGF-I is associated with fitness and health outcomes in a population of 846 young healthy men. Growth Horm IGF Res. 2011 Jun;21(3):124-8. doi: 10.1016/j.ghir.2011.03.001. Epub 2011 Apr 2. PMID: 21459641.
4. Puche JE, Castilla-Cortázar I. Human conditions of insulin-like growth factor-I (IGF-I) deficiency. J Transl Med. 2012 Nov 14;10:224. doi: 10.1186/1479-5876-10-224. PMID: 23148873; PMCID: PMC3543345.
5. Rommel C, Bodine SC, Clarke BA, Rossman R, Nunez L, Stitt TN, Yancopoulos GD, Glass DJ. Mediation of IGF-1-induced skeletal myotube hypertrophy by PI(3)K/Akt/mTOR and PI(3)K/Akt/GSK3 pathways. Nat Cell Biol. 2001 Nov;3(11):1009-13. doi: 10.1038/ncb1101-1009. PMID: 11715022.
6. Peng XD, Xu PZ, Chen ML, Hahn-Windgassen A, Skeen J, Jacobs J, Sundararajan D, Chen WS, Crawford SE, Coleman KG, Hay N. Dwarfism, impaired skin development, skeletal muscle atrophy, delayed bone development, and impeded adipogenesis in mice lacking Akt1 and Akt2. Genes Dev. 2003 Jun 1;17(11):1352-65. doi: 10.1101/gad.1089403. PMID: 12782654; PMCID: PMC196068.
7. Bodine SC, Latres E, Baumhueter S, Lai VK, Nunez L, Clarke BA, Poueymirou WT, Panaro FJ, Na E, Dharmarajan K, Pan ZQ, Valenzuela DM, DeChiara TM, Stitt TN, Yancopoulos GD, Glass DJ. Identification of ubiquitin ligases required for skeletal muscle atrophy. Science. 2001 Nov 23;294(5547):1704-8. doi: 10.1126/science.1065874. Epub 2001 Oct 25. PMID: 11679633..
8. Sahebkar A. Are curcuminoids effective C-reactive protein-lowering agents in clinical practice? Evidence from a meta-analysis. Phytother Res. 2014 May;28(5):633-42. doi: 10.1002/ptr.5045. Epub 2013 Aug 7. PMID: 23922235..
9. Mohammadi A, Sahebkar A, Iranshahi M, Amini M, Khojasteh R, Ghayour-Mobarhan M, Ferns GA. Effects of supplementation with curcuminoids on dyslipidemia in obese patients: a randomized crossover trial. Phytother Res. 2013 Mar;27(3):374-9. doi: 10.1002/ptr.4715. Epub 2012 May 21. PMID: 22610853..
10. Hedayati S, Riyahi Malayeri S, Hoseini M. The Effect of Eight Weeks of High and Moderate Intensity Interval Training Along with Aloe Vera Consumption on Serum Levels of Chemerin, Glucose and Insulin in Streptozotocin-induced Diabetic Rats: An Experimental Study. JRUMS 2018; 17 (9) :801-814. URL: http://journal.rums.ac.ir/article-1-4209-en.html.
11. Hosseini M, Eftekhar B, Riyahi Malayeri S. Effect of Interval Training with Curcumin Consumption on Some Adipokines in Menopausal Obese Rats. JRUMS 2017; 16 (6) :505-516. URL: http://journal.rums.ac.ir/article-1-3644-en.html.
12. Riyahi Malayeri S, Abdolhay S, Behdari R, Hoseini M. The combined effect of resveratrol supplement and endurance training on IL-10 and TNF- α in type 2 diabetic rats. RJMS 2019; 25 (12) :140-149. URL: http://rjms.iums.ac.ir/article-1-5526-en.html
13. Arabzadeh E, Shirvani H, Ebadi Zahmatkesh M, Riyahi Malayeri S, Meftahi GH, Rostamkhani F. Irisin/FNDC5 influences myogenic markers on skeletal muscle following high and moderate-intensity exercise training in STZ-diabetic rats. 3 Biotech. 2022 Sep;12(9):193. doi: 10.1007/s13205-022-03253-9. Epub 2022 Jul 26. PMID: 35910290; PMCID: PMC9325938.
14. Osali A. Effect of six -week aerobic exercise and consumption of nanocurcumin on TNF-α and memory in 60-65 years old women with metabolic syndrome. Koomesh. 2018; 20 (3) :503-509.
15. El-Bahr S M. Curcumin regulates gene expression of insulin like growth factor, B-cell CLL/lymphoma 2 and antioxidant enzymes in streptozotocin induced diabetic rats. BMC complementary and alternative medicine 2013; 13(1): 1-11.
16. Rashidi E, Hosseini Kakhak S A R, Askari R. The Effect of 8 Weeks Resistance Training With Low Load and High Load on Testosterone, Insulin-like Growth Factor-1, Insulin-like Growth Factor Binding Protein-3 Levels, and Functional Adaptations in Older Women. Salmand: Iranian Journal of Ageing 2019; 14 (3) :356-367. URL: http://salmandj.uswr.ac.ir/article-1-1498-en.html.
17. Vakili J, Sari Sarraf V, Khanvari T. Effects of High-intensity Interval Training on Body Composition and Hormone Growth Agents in Overweight Adolescent Boys. J Arak Uni Med Sci 2021; 24 (1) :136-149.
18. Sarmadiyan M, Khorshidi D. Effect of combined training on body composition, lipids levels and indicators of metabolic syndrome in overweight and obesed postmenopausal women. joge 2016; 1 (2) :36-44
URL: http://joge.ir/article-1-100-en.html
