Effect of alfaalfa (Medicago sativa) extract on spatial memory and anxiety like behaviors of ovariectomized rats
Subject Areas :Hadi Semizeh 1 , Seyed Reza Fatemi Tabatabaei 2 , Naeem Erfanimajd 3 , Ali Shahriari 4
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Abstract :
Introduction: According to the high phytoestrogens content of alfalfa and the effect of phytoestrogens on CNS, the effect of ethyl acetate extract of alfalfa on spatial memory and anxiety like behaviors of ovariectomized rats were accessed. Methods: Forty-five female Wistar rats (three-month old) were accidentally divided into 5 groups, including sham, ovariectomy (ovx), sham+4 (4g/kg extract in diet), ovx+2 (2g/kg extract in diet) and ovx+4 (4g/kg extract in diet) two weeks after ovariectomy or sham operation. Memory and anxiety like behavior were elevate using Morris water maze and plus maze, respectively. MDA and antioxidant enzymes were also measured in hippocampus. Results: concentration of estrogen in plasma and superoxide dismutase activity (SOD) in hippocampus were reduced in ovx group, but consumption of alfalfa extract increased SOD activity while decreased catalase activity. Performance of ovx+2 group was decreased in training trial of Morris water maze, while consumption of alfalfa extract in ovariectomized rat increased percentage of open arm time (OAT%) in elevated plus maze. Conclusion: The findings revealed that the five-factor model of the Mc health-related quality of life questionnaire (SF-36) has satisfactory validity and reliability. Thus, this questionnaire can be used in future studies to assess the quality of life of patient’s type 2 diabetes.
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