Effect of probiotic supplementation with aerobic training on hepatic IL-6 gene expression in non-alcoholic fatty liver rats
Subject Areas : Sport Nutrition
Masoumeh Hosseini
1
,
Zahra Tootoonchi
2
1 - Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.
2 - Department of Physical Education and Sports Sciences, East Tehran Branch, Islamic Azad University Tehran, Iran.
Keywords: Aerobic Training, Probiotic, IL 6, Non-alcoholic Fatty liver,
Abstract :
Background: non-alcoholic fatty liver disease NAFLD is the most common liver disease in the world, the most important cause of non-alcoholic fatty liver disease is poor eating habits and sedentary lifestyles that cause obesity. The aim of this study was the effect of aerobic training and probiotic consumption on gene expression of IL6 liver tissue in rats with non-alcoholic fatty liver. Materials and Methods: In this experimental study, 32 male Wistar rats, 200-250 gr, were randomly divided into 4 groups: healthy group, modeled group (steatosis), steatosis + probiotic group, steatosis + probiotic + training group. To create a fatty liver model, tetracycline at a dose of 100 mg / kg at a volume of 1.5 cc per rat was gavaged daily for two weeks. The main training program was for eight weeks/3times per week. In the first week with a speed of 18 meters per minute, the time started 10 minutes and every week quickly, 1-2 meters per minute and time was added to 10 minutes so that in the fourth week the speed to 22 meters per minute and time to 40 minute has arrived. Probiotic groups received 109 CFU / ml of Lactobacillus ramensus by gavage daily for 8 weeks and 5 days a week. 48 hours after the last training session, 10 ml of blood was taken directly from the hearts of rat to test for total cholesterol (TC) and liver tissue samples were taken to test for IL6 gene expression. One-way analysis of variance and Tukey post hoc test was used P≤0.05. Results: There is a significant difference between the IL-6 gene variable in the groups (P=0/000), Induction of fatty liver in rats was associated with a significant increase in interleukin-6 gene levels, these changes were significantly reduced after taking probiotics (P=0/000), Also probiotic consumption with aerobic training had a significant reduction compared to other groups. However taking probiotic with aerobic training had no significant effect on TC of rats with non-alcoholic fatty liver (P=0/306). Conclusion: Probably, performing aerobic training along with taking probiotic can have a better effect in controlling non-alcoholic fatty liver disease.
1. Marchesini G, Marzocchi R, Agostini F, Bugianesi E. Nonalcoholic fatty liver disease and the metabolic syndrome. Curr Opin Lipidol. 2005 Aug;16(4):421-7. doi: 10.1097/01.mol.0000174153.53683.f2. PMID: 15990591.
2. Nobili V, Marcellini M, Devito R, Ciampalini P, Piemonte F, Comparcola D, Sartorelli MR, Angulo P. NAFLD in children: a prospective clinical-pathological study and effect of lifestyle advice. Hepatology. 2006 Aug;44(2):458-65. doi: 10.1002/hep.21262. PMID: 16871574.
3. Arabzadeh E, Shirvani H, Ebadi Zahmatkesh M, Riyahi Malayeri S, Meftahi GH, Rostamkhani F. Irisin/FNDC5 influences myogenic markers on skeletal muscle following high and moderate-intensity exercise training in STZ-diabetic rats. 3 Biotech. 2022 Sep;12(9):193. doi: 10.1007/s13205-022-03253-9. Epub 2022 Jul 26. PMID: 35910290; PMCID: PMC9325938.
4. Nigam P, Bhatt SP, Misra A, Vaidya M, Dasgupta J, Chadha DS. Non-alcoholic fatty liver disease is closely associated with sub-clinical inflammation: a case-control study on Asian Indians in North India. PLoS One. 2013;8(1):e49286. doi: 10.1371/journal.pone.0049286. Epub 2013 Jan 11. PMID: 23326306; PMCID: PMC3543427.
5. Shirvani, H., Riayhi malayeri, S., Akbarpour Bani, M., Kazemzadeh, Y. The Effects of Taurine Supplementation with High Intensity Intermittent Exercise on Serum IL-6 and TNF-α in Well-Trained Soccer Players. Journal of Sport Biosciences, 2013; 5(2): 59-79. doi: 10.22059/jsb.2013.35040. https://jsb.ut.ac.ir/article_35040.html?lang=en .
6. Fischer CP. Interleukin-6 in acute exercise and training: what is the biological relevance? Exerc Immunol Rev. 2006;12:6-33. PMID: 17201070.
7. Huh JY. The role of exercise-induced myokines in regulating metabolism. Arch Pharm Res. 2018 Jan;41(1):14-29. doi: 10.1007/s12272-017-0994-y. Epub 2017 Nov 25. PMID: 29177585.
8. Gómez-Rubio P, Trapero I. The Effects of Exercise on IL-6 Levels and Cognitive Performance in Patients with Schizophrenia. Diseases. 2019 Jan 22;7(1):11. doi: 10.3390/diseases7010011. PMID: 30678202; PMCID: PMC6473765.
9. Cai GS, Su H, Zhang J. Protective effect of probiotics in patients with non-alcoholic fatty liver disease. Medicine (Baltimore). 2020 Aug 7;99(32):e21464. doi: 10.1097/MD.0000000000021464. PMID: 32769877; PMCID: PMC7592998.
10. Hertz CJ, Kiertscher SM, Godowski PJ, Bouis DA, Norgard MV, Roth MD, Modlin RL. Microbial lipopeptides stimulate dendritic cell maturation via Toll-like receptor 2. J Immunol. 2001 Feb 15;166(4):2444-50. doi: 10.4049/jimmunol.166.4.2444. PMID: 11160304.
11. Tang Y, Huang J, Zhang WY, Qin S, Yang YX, Ren H, Yang QB, Hu H. Effects of probiotics on nonalcoholic fatty liver disease: a systematic review and meta-analysis. Therap Adv Gastroenterol. 2019 Sep 25;12:1756284819878046. doi: 10.1177/1756284819878046. PMID: 31598135; PMCID: PMC6764034.
12. Rahimlou M, Hosseini SA, Majdinasab N, Haghighizadeh MH, Husain D. Effects of long-term administration of Multi-Strain Probiotic on circulating levels of BDNF, NGF, IL-6 and mental health in patients with multiple sclerosis: a randomized, double-blind, placebo-controlled trial. Nutr Neurosci. 2022 Feb;25(2):411-422. doi: 10.1080/1028415X.2020.1758887. Epub 2020 Jun 5. PMID: 32500827.
13. Hosseini M, Ghasem Zadeh Khorasani N, Divkan B, Riyahi Malayeri S. Interactive Effect of High Intensity Interval Training with Vitamin E Consumption on the Serum Levels of Hsp70 and SOD in Male Wistar Rats. Iranian J Nutr Sci Food Technol 2019; 13 (4) :21-28. URL: http://nsft.sbmu.ac.ir/article-1-2689-en.html.
14. Le T, Hekmat S. Development of pulse-based probiotics by fermentation using Fiti sachets for the developing world. Nutrition & Food Science. 2020 Mar 3;50(6):1109-21. https://doi.org/10.1108/NFS-08-2019-0272
15. Pinto AP, da Rocha AL, Cabrera EMB, et al. Role of interleukin-6 in inhibiting hepatic autophagy markers in exercised mice. Cytokine. 2020 Apr;130:155085. DOI: 10.1016/j.cyto.2020.155085. PMID: 32259772.
16. Rong YD, Bian AL, Hu HY, Ma Y, Zhou XZ. Study on relationship between elderly sarcopenia and inflammatory cytokine IL-6, anti-inflammatory cytokine IL-10. BMC Geriatr. 2018 Dec 12;18(1):308. doi: 10.1186/s12877-018-1007-9. PMID: 30541467; PMCID: PMC6292155.
17. Zheng G, Qiu P, Xia R, Lin H, Ye B, Tao J, Chen L. Effect of Aerobic Exercise on Inflammatory Markers in Healthy Middle-Aged and Older Adults: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Front Aging Neurosci. 2019 Apr 26;11:98. doi: 10.3389/fnagi.2019.00098. PMID: 31080412; PMCID: PMC6497785.
18. Riyahi Malayeri, S., Mirakhorli, M. The Effect of 8 Weeks of Moderate Intensity Interval Training on Omentin Levels and Insulin Resistance Index in Obese Adolescent Girls. Sport Physiology & Management Investigations, 2018; 10(2): 59-68. https://www.sportrc.ir/article_67070.html?lang=en.
19. Wedell-Neergaard AS, Lang Lehrskov L, Christensen RH, Legaard GE, Dorph E, Larsen MK, Launbo N, Fagerlind SR, Seide SK, Nymand S, Ball M, Vinum N, Dahl CN, Henneberg M, Ried-Larsen M, Nybing JD, Christensen R, Rosenmeier JB, Karstoft K, Pedersen BK, Ellingsgaard H, Krogh-Madsen R. Exercise-Induced Changes in Visceral Adipose Tissue Mass Are Regulated by IL-6 Signaling: A Randomized Controlled Trial. Cell Metab. 2019 Apr 2;29(4):844-855.e3. doi: 10.1016/j.cmet.2018.12.007. Epub 2018 Dec 27. PMID: 30595477.
20. Albaghdadi MS, Wang Z, Gao Y, Mutharasan RK, Wilkins J. High-Density Lipoprotein Subfractions and Cholesterol Efflux Capacity Are Not Affected by Supervised Exercise but Are Associated with Baseline Interleukin-6 in Patients with Peripheral Artery Disease. Front Cardiovasc Med. 2017 Mar 2;4:9. doi: 10.3389/fcvm.2017.00009. PMID: 28303243; PMCID: PMC5332379.
21. Kocsis T, Molnár B, Németh D, Hegyi P, Szakács Z, Bálint A, Garami A, Soós A, Márta K, Solymár M. Probiotics have beneficial metabolic effects in patients with type 2 diabetes mellitus: a meta-analysis of randomized clinical trials. Sci Rep. 2020 Jul 16;10(1):11787. doi: 10.1038/s41598-020-68440-1. PMID: 32678128; PMCID: PMC7366719.
22. Amaral AL, Mendonça AM, Giolo JS, Costa JG, Mariano IM, de Souza TCF, Batista JP, Rodrigues ML, de Souza AV, Caixeta DC, Peixoto LG, de Oliveira EP, Espindola FS, Puga GM. The effects of isoflavone supplementation plus combined exercise on salivary markers of oxidative stress in postmenopausal women. J Clin Biochem Nutr. 2020 Jan;66(1):43-48. doi: 10.3164/jcbn.19-44. Epub 2019 Oct 31. PMID: 32001955; PMCID: PMC6983439.
23. Barzegar H, Vosadi E, Borjian Fard M. The Effect of Different Modes of Training on Plasma Levels of IL-6 and IL-10 in Male Mature Wistar Rats. Journal of Sport Biosciences. 2017 Aug 23;9(2):171-81. https://doi.org/10.22059/jsb.2017.98145.726
24. Loman BR, Hernández-Saavedra D, An R, Rector RS. Prebiotic and probiotic treatment of nonalcoholic fatty liver disease: a systematic review and meta-analysis. Nutr Rev. 2018 Nov 1;76(11):822-839. doi: 10.1093/nutrit/nuy031. PMID: 30113661.
25. Comassi M, Vitolo E, Pratali L, Del Turco S, Dellanoce C, Rossi C, Santini E, Solini A. Acute effects of different degrees of ultra-endurance exercise on systemic inflammatory responses. Intern Med J. 2015 Jan;45(1):74-9. doi: 10.1111/imj.12625. PMID: 25371101.
26. Wang L, Guo MJ, Gao Q, Yang JF, Yang L, Pang XL, Jiang XJ. The effects of probiotics on total cholesterol: A meta-analysis of randomized controlled trials. Medicine (Baltimore). 2018 Feb;97(5):e9679. doi: 10.1097/MD.0000000000009679. PMID: 29384846; PMCID: PMC5805418.
27. Pourrajab B, Fatahi S, Dehnad A, Kord Varkaneh H, Shidfar F. The impact of probiotic yogurt consumption on lipid profiles in subjects with mild to moderate hypercholesterolemia: A systematic review and meta-analysis of randomized controlled trials. Nutr Metab Cardiovasc Dis. 2020 Jan 3;30(1):11-22. doi: 10.1016/j.numecd.2019.10.001. Epub 2019 Oct 11. PMID: 31748179.
28. Wu Y, Zhang Q, Ren Y, Ruan Z. Effect of probiotic Lactobacillus on lipid profile: A systematic review and meta-analysis of randomized, controlled trials. PLoS One. 2017 Jun 8;12(6):e0178868. doi: 10.1371/journal.pone.0178868. PMID: 28594860; PMCID: PMC5464580.
29. Sivamaruthi BS, Kesika P, Chaiyasut C. A mini-review of human studies on cholesterol-lowering properties of probiotics. Scientia Pharmaceutica. 2019;87(4):26. https://doi.org/10.3390/scipharm87040026.
30. Jensen CS, Musaeus CS, Frikke-Schmidt R, Andersen BB, Beyer N, Gottrup H, Høgh P, Vestergaard K, Wermuth L, Frederiksen KS, Waldemar G, Hasselbalch S, Simonsen AH. Physical Exercise May Increase Plasma Concentration of High-Density Lipoprotein-Cholesterol in Patients With Alzheimer's Disease. Front Neurosci. 2020 May 27;14:532. doi: 10.3389/fnins.2020.00532. PMID: 32536853; PMCID: PMC7269030.
31. Zhang J, Miao G, Cao JM, Zhou HT, Niu YL, Zhang Y, Ren Y, Bao XY, Xing YW. [Effects of aerobic exercise combined with chlorella pyrenoidos of disintegrated cell wall on some indicators of lipid metabolism in rats with high-fat diet]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2018 May 8;34(5):445-449. Chinese. doi: 10.12047/j.cjap.5688.2018.101. PMID: 30788927.
