Influence of Β-Hydroxy-Β-Methyl Butyrate Supplementation on Strength, Muscle and Liver-Damage Indices Induced by Dual Pyramid Resistance Training in Beginner Bodybuilders
Subject Areas : Sport Nutrition
Soleyman Ansari
1
(PhD -Exercise Physiology, Department of Physical Education, Rasht Branch, Islamic Azad University, Rasht, Iran)
Shahram Gholamrezaei
2
(Assistant Professor, Department of Physical Education, Rasht Branch, Islamic Azad University, Rasht, Iran)
Fahimeh AdibSaber
3
(Assistant Professor, Department of Physical Education, Rasht Branch, Islamic Azad University, Rasht, Iran)
Mohammad Moradnia
4
(MA in Exercise Physiology, Department of Physical Education, Faculty of Humanities, Islamic Azad University, Rasht Branch, Rasht, Iran)
Keywords: β-Hydroxy-β-Methyl butyrate, muscle strength, liver-damage markers, muscle-damage indices, dual pyramid resistance training,
Abstract :
Background: It has been suggested that ingesting supplements decrease muscle soreness, increase muscle strength, hypertrophy, and health-related indices. This study investigated the influence of 8-week β-Hydroxy-β-Methyl butyrate (HMB) supplementation combined with a dual pyramid resistance exercise program on strength, muscle, and liver-damage indices (ALT, AST, CK, and LDH) and body mass index (BMI) in beginner bodybuilders. Materials and Methods: A total of 40 beginner bodybuilders was randomized to an intervention group (resistance training + HMB supplementation) and a control group (resistance training + placebo) for 8 weeks. Blood samples and body measurements were taken at baseline and after the end of the intervention. The subjects were evaluated for BMI, 1 repetition maximum (1RM) bench press and leg press prior to and after the training intervention. In addition, blood samples were obtained before and after 8-week resistance training to evaluate creatine kinase (CK), lactate dehydrogenase (LD), aspartate aminotransferase (AST), and alanine aminotransferase (Johnson et al.) responses. Results: After 8 weeks of HMB ingestion, serum levels of CK (p<0.001), LDH (p<0.014), and ALT (p<0.009) of participants in the experimental group significantly decreased compared to the placebo group. Furthermore, The HMB group showed greater gains compared with the placebo group in 1RM leg and bench press (p<0.001). Regarding BMI and AST serum levels, there were no significant differences between groups. Conclusion: The results of the present study showed that HMB supplementation may attenuate the resistance exercise-induced muscle and liver damage indices and have beneficial effects on muscle strength.
1. Fleck SJ, Kraemer W. Designing resistance training programs, 4E: Human Kinetics; 2014.
2. Shirato, M., Tsuchiya, Y., Sato, T. et al. Effects of combined β-hydroxy-β-methylbutyrate (HMB) and whey protein ingestion on symptoms of eccentric exercise-induced muscle damage. J Int Soc Sports Nutr 13, 7 (2016). https://doi.org/10.1186/s12970-016-0119-x
3. Bompa T, Buzzichelli C. Periodization training for sports, 3e: Human kinetics; 2015.
4. Kirby TJ, Triplett NT, Haines TL, Skinner JW, Fairbrother KR, McBride JM. Effect of leucine supplementation on indices of muscle damage following drop jumps and resistance exercise. Amino Acids. 2012 May;42(5):1987-96. doi: 10.1007/s00726-011-0928-9. Epub 2011 May 12. PMID: 21562819.
5. Cooke MB, Rybalka E, Williams AD, Cribb PJ, Hayes A. Creatine supplementation enhances muscle force recovery after eccentrically-induced muscle damage in healthy individuals. J Int Soc Sports Nutr. 2009 Jun 2;6:13. doi: 10.1186/1550-2783-6-13. PMID: 19490606; PMCID: PMC2697134.
6. R Pincus M, A McPherson R. Henry’s Clinical Diagnosis and Management by Laboratory Methods, 23e. Elsevier; 2017.
7. Johnson NA, Sachinwalla T, Walton DW, Smith K, Armstrong A, Thompson MW, George J. Aerobic exercise training reduces hepatic and visceral lipids in obese individuals without weight loss. Hepatology. 2009 Oct;50(4):1105-12. doi: 10.1002/hep.23129. PMID: 19637289.
8. Pettersson J, Hindorf U, Persson P, Bengtsson T, Malmqvist U, Werkström V, Ekelund M. Muscular exercise can cause highly pathological liver function tests in healthy men. Br J Clin Pharmacol. 2008 Feb;65(2):253-9. doi: 10.1111/j.1365-2125.2007.03001.x. Epub 2007 Aug 31. PMID: 17764474; PMCID: PMC2291230.
9. Brancaccio P, Lippi G, Maffulli N. Biochemical markers of muscular damage. Clin Chem Lab Med. 2010 Jun;48(6):757-67. doi: 10.1515/CCLM.2010.179. PMID: 20518645.
10. Durkalec-Michalski K, Jeszka J. The efficacy of a β-hydroxy-β-methylbutyrate supplementation on physical capacity, body composition and biochemical markers in elite rowers: a randomised, double-blind, placebo-controlled crossover study. J Int Soc Sports Nutr. 2015 Jul 30;12:31. doi: 10.1186/s12970-015-0092-9. PMID: 26225130; PMCID: PMC4518594.
11. Wilson JM, Kim JS, Lee SR, Rathmacher JA, Dalmau B, Kingsley JD, Koch H, Manninen AH, Saadat R, Panton LB. Acute and timing effects of beta-hydroxy-beta-methylbutyrate (HMB) on indirect markers of skeletal muscle damage. Nutr Metab (Lond). 2009 Feb 4;6:6. doi: 10.1186/1743-7075-6-6. PMID: 19193206; PMCID: PMC2642830.
12. Wilson JM, Lowery RP, Joy JM, Andersen JC, Wilson SM, Stout JR, Duncan N, Fuller JC, Baier SM, Naimo MA, Rathmacher J. The effects of 12 weeks of beta-hydroxy-beta-methylbutyrate free acid supplementation on muscle mass, strength, and power in resistance-trained individuals: a randomized, double-blind, placebo-controlled study. Eur J Appl Physiol. 2014 Jun;114(6):1217-27. doi: 10.1007/s00421-014-2854-5. Epub 2014 Mar 6. PMID: 24599749; PMCID: PMC4019830..
13. Nunes EA, Lomax AR, Noakes PS, Miles EA, Fernandes LC, Calder PC. β-Hydroxy-β-methylbutyrate modifies human peripheral blood mononuclear cell proliferation and cytokine production in vitro. Nutrition. 2011 Jan;27(1):92-99. doi: 10.1016/j.nut.2009.12.008. Epub 2010 Jun 11. PMID: 20541366.
14. Ellis AC, Hunter GR, Goss AM, Gower BA. Oral Supplementation with Beta-Hydroxy-Beta-Methylbutyrate, Arginine, and Glutamine Improves Lean Body Mass in Healthy Older Adults. J Diet Suppl. 2019;16(3):281-293. doi: 10.1080/19390211.2018.1454568. Epub 2018 Apr 19. PMID: 29672184; PMCID: PMC6314919..
15. Panton LB, Rathmacher JA, Baier S, Nissen S. Nutritional supplementation of the leucine metabolite beta-hydroxy-beta-methylbutyrate (hmb) during resistance training. Nutrition. 2000 Sep;16(9):734-9. doi: 10.1016/s0899-9007(00)00376-2. PMID: 10978853.
16. Faul F, Erdfelder E, Lang AG, Buchner A. G*Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods. 2007 May;39(2):175-91. doi: 10.3758/bf03193146. PMID: 17695343.
17. Asadi A, Arazi H, Suzuki K. Effects of β-Hydroxy-β-methylbutyrate-free Acid Supplementation on Strength, Power and Hormonal Adaptations Following Resistance Training. Nutrients. 2017 Dec 2;9(12):1316. doi: 10.3390/nu9121316. PMID: 29207472; PMCID: PMC5748766.
18. Bompa T, Di Pasquale M, Cornacchia L. Serious strength training: Human Kinetics; 2012.
19. Medicine ACoS. ACSM's resource manual for guidelines for exercise testing and prescription: Lippincott Williams & Wilkins; 2012.
20. ARAZI, H., & ROHANI, H., & GHIASI, A., & DAVARAN, M. (2015). THE EFFECT OF HMB SUPPLEMENTATION ON CARDIOVASCULAR RISK FACTORS AFTER FOUR WEEKS OF RESISTANCE TRAINING IN AMATEUR ATHLETES. INTERNATIONAL CARDIOVASCULAR RESEARCH JOURNAL, 9(2), 89-93. https://www.sid.ir/en/journal/ViewPaper.aspx?id=436571
21. Cohen J. Statistical power analysis for the behavioral sciences: Academic press; 2013.
22. Asjodi F, Izadi A. The Effects of 8 weeks beta-hydroxy-beta-methylbutyrate (HMB) supplementation on body composition, inflammatory response and muscle damage after eccentric exercise in untrained males. Progress In Nutrition. 2019;21:184-90. https://doi.org/10.23751/pn.v21i1-S.5883
23. Nissen S, Sharp R, Ray M, Rathmacher JA, Rice D, Fuller JC Jr, Connelly AS, Abumrad N. Effect of leucine metabolite beta-hydroxy-beta-methylbutyrate on muscle metabolism during resistance-exercise training. J Appl Physiol (1985). 1996 Nov;81(5):2095-104. doi: 10.1152/jappl.1996.81.5.2095. PMID: 8941534.
24. van Someren KA, Edwards AJ, Howatson G. Supplementation with beta-hydroxy-beta-methylbutyrate (HMB) and alpha-ketoisocaproic acid (KIC) reduces signs and symptoms of exercise-induced muscle damage in man. Int J Sport Nutr Exerc Metab. 2005 Aug;15(4):413-24. doi: 10.1123/ijsnem.15.4.413. PMID: 16286672.
25. Saki B, Gaeini AA, Choubineh S. The Effects of Short-Term β-Hydroxy-β-Methylbutyrate Supplementation on Serum AST, ALT, AP, and Urea Levels after Intense Resistance Exercise in Untrained Male Students. Journal of Isfahan Medical School. 2012;30(190).
26. Nissen S, Sharp RL, Panton L, Vukovich M, Trappe S, Fuller JC Jr. beta-hydroxy-beta-methylbutyrate (HMB) supplementation in humans is safe and may decrease cardiovascular risk factors. J Nutr. 2000 Aug;130(8):1937-45. doi: 10.1093/jn/130.8.1937. PMID: 10917905..
27. Tritto AC, Bueno S, Rodrigues RMP, Gualano B, Roschel H, Artioli GG. Negligible Effects of β-Hydroxy-β-Methylbutyrate Free Acid and Calcium Salt on Strength and Hypertrophic Responses to Resistance Training: A Randomized, Placebo-Controlled Study. Int J Sport Nutr Exerc Metab. 2019 Sep 1;29(5):505-511. doi: 10.1123/ijsnem.2018-0337. PMID: 30859862..
28. Lamboley CR, Royer D, Dionne IJ. Effects of beta-hydroxy-beta-methylbutyrate on aerobic-performance components and body composition in college students. Int J Sport Nutr Exerc Metab. 2007 Feb;17(1):56-69. doi: 10.1123/ijsnem.17.1.56. PMID: 17469236..
29. Flakoll P, Sharp R, Baier S, Levenhagen D, Carr C, Nissen S. Effect of beta-hydroxy-beta-methylbutyrate, arginine, and lysine supplementation on strength, functionality, body composition, and protein metabolism in elderly women. Nutrition. 2004 May;20(5):445-51. doi: 10.1016/j.nut.2004.01.009. PMID: 15105032..
30. Jówko E, Ostaszewski P, Jank M, Sacharuk J, Zieniewicz A, Wilczak J, Nissen S. Creatine and beta-hydroxy-beta-methylbutyrate (HMB) additively increase lean body mass and muscle strength during a weight-training program. Nutrition. 2001 Jul-Aug;17(7-8):558-66. doi: 10.1016/s0899-9007(01)00540-8. PMID: 11448573..
