Review the effect of eight weeks of aerobic training with gallic acid on tumor necrosis factor alpha and nuclear transcription factor kappa B in prostate tissue of rats with prostate cancer
الموضوعات : Sport Physiology
Sayeh Toutounchi Avval
1
,
Mohammad Ali Azarbayjani
2
,
Seyed Ali Hosseini
3
,
Shahram Soheili
4
,
Rouhallah Ershadi
5
1 - Department of Physical Education and Sport Science, Ki.C., Islamic Azad University, Kish, Iran
2 - Department of Physical Education and Sport Sciences, CT.C., Islamic Azad University, Tehran, Iran
3 - Department of Sport Physiology, Marv.C, Islamic Azad University, Marvdasht, Iran
4 - Department of Physical Education and Sport Science, ShQ.C., Islamic Azad University, Shahr-e Qods, Iran
5 - Department of Physical Education and sport science, Bu.C., Islamic Azad University, Bushehr, Iran
الکلمات المفتاحية: Exercise, Gallic Acid, Inflammation, Prostate Cancer,
ملخص المقالة :
Introduction: Increased inflammation in cancer-prone tissue can lead to faster progression and damage to that tissue. Although the role of exercise and antioxidants in cancer improvement has been reported, their interactive effect is not yet well understood. Therefore, the aim of the present study was to investigate the effect of eight weeks of aerobic training (AT) with gallic acid (GA) on tumor necrosis factor alpha (TNF-α) and nuclear transcription factor kappa B (NF-kB) levels in prostate tissue of rats with prostate cancer (PC).
Methods: 40 male rats (age 10- 12 months and weight 260- 300 g) with prostate cancer (with the LNCaP cell line) were randomly assigned to the following groups: 1) cancer control (PCa), 2) sham (solvent of GA/(Sh)), 3) GA, 4) AT, and 5) AT+GA. Also, to investigate the effect of PC induction on the research variables, 8 healthy rats were assigned to the healthy control (HC) group. The AT groups ran three sessions per week at an intensity of 15 m/min on a 15 degree incline for 30-60 minutes for eight weeks. The GA groups also consumed daily 20 mg/kg GA orally.
Results: The results showed that in the AT, GA and AT+GA groups, the levels of TNF-α and NF-kB were significantly lower than PCa group. The levels of TNF-α and NF-kB in the AT group were significantly lower than GA group. Also, in the AT+GA group, they were significantly lower than AT and GA groups.
Conclusion: It seems that although AT and GA alone have a reducing effect on some inflammatory markers. However, these two (AT and GA) can enhance each other's effect on reducing inflammatory markers in prostate tissue following PC.
Brown, J. C., Compton, S. L., Kang, A., Jayaraman, A., Gilmore, L. A., Kirby, B. J., ... & Spielmann, G. (2025). Effects of exercise on inflammation, circulating tumor cells, and circulating tumor DNA in colorectal cancer. Journal of Sport and Health Science, 101036.
Chen, X., Zhang, J., Gao, F., Liu, N., Du, H., Li, J., ... & Chen, R. (2025). Exercise therapy: an effective approach to mitigate the risk of cancer metastasis. World Journal of Surgical Oncology, 23(1), 1-20.
Chu, F., Chen, L., Guan, Q., Chen, Z., Ji, Q., Ma, Y.,. & Zheng, Y. (2025). Global burden of prostate cancer: age-period-cohort analysis from 1990 to 2021 and projections until 2040. World Journal of Surgical Oncology, 23(1), 98.
Donate-Moreno, M. J., Lorenzo-Sánchez, M. V., Migallón, I. D. D. M. S., Herraiz-Raya, L., Esper-Rueda, J. A., Legido-Gómez, O., ... & Salinas-Sánchez, A. S. (2020). Inflammatory markers as prognostic factors in metastatic castration-resistant prostate cancer. Actas Urológicas Españolas (English Edition), 44(10), 692-700.
Driscoll, C., Handa, N., Hao, Y., Alshalalfa, M., Hakansson, A. K., Proudfoot, J. A., ... & Kundu, S. D. (2025). Tumor necrosis factor alpha signaling activity and NCCN risk, adverse pathology, and progression during active surveillance in prostate cancer.
Ji, L. L. (2025). Nuclear Factor κB Signaling Revisited: Its Role in Skeletal Muscle and Exercise. Free Radical Biology and Medicine.
Jiang, Y., Pei, J., Zheng, Y., Miao, Y. J., Duan, B. Z., & Huang, L. F. (2022). Gallic acid: A potential anti-cancer agent. Chinese journal of integrative medicine, 28(7), 661-671.
Kang, D. Y., Bae, S. W., & Jang, K. J. (2025). Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells. Molecular Medicine Reports, 31(6), 151.
Liu, B., Tian, H., & Momeni, M. R. (2025). The interplay of exercise and green tea: a new road in cancer therapy. Cancer Cell International, 25(1), 1-23.
McCullough, D. J., Nguyen, L. M. D., Siemann, D. W., & Behnke, B. J. (2013). Effects of exercise training on tumor hypoxia and vascular function in the rodent preclinical orthotopic prostate cancer model. Journal of applied physiology, 115(12), 1846-1854.
Naghizadeh, N., Riyahi Malayeri, S., Roozbahani, M., Khademi, A., Shirvani, H. The Effectiveness of Endurance Training and Nano Curcumin Supplementation on the Expression of Mir-21and P53 Genes in Brain Tumor Tissue in an Animal Model of Glioblastoma Multiform. Journal of Nutrition,Fasting and Health, 2024; 12(1): 26-35. doi: 10.22038/jnfh.2023.73964.1455
Nowroozi, M. R., Ohadian Moghadam, S., Amini, E., Nowroozi, A., Ayati, M., & Jamshidian, H. (2017). Infection, inflammation and prostate cancer. Journal of Medical Council of Iran, 35(3), 233-239.
Ojo, O. A., Rotimi, D. E., Ojo, A. B., Ogunlakin, A. D., & Ajiboye, B. O. (2023). Gallic acid abates cadmium chloride toxicity via alteration of neurotransmitters and modulation of inflammatory markers in Wistar rats. Scientific Reports, 13(1), 1577.
Pang, H., & Badehnoosh, B. (2025). Synergistic strength: unleashing exercise and polyphenols against breast cancer. Cancer Cell International, 25(1), 144.
Parastesh, M., Bayatiani, M., Khaleghi, M., Sadeghian Shahi, M., Ayatizadeh Tafti, F., & Aria, B. (2022). The effect of high-intensity interval training on inflammatory markers in male rats undergoing X-ray radiation. J Bas Res Med Sci, 9(1), 10-8.
Rebillard, A., Lefeuvre-Orfila, L., Gueritat, J., & Cillard, J. (2013). Prostate cancer and physical activity: adaptive response to oxidative stress. Free radical biology and medicine, 60, 115-124.
Saif-Elnasr, M., El-Ghlban, S., Bayomi, A. I., El-Sayyad, G. S., & Maghraby, M. S. (2023). Gallic acid and/or cerium oxide nanoparticles synthesized by gamma-irradiation protect cisplatin-induced nephrotoxicity via modulating oxidative stress, inflammation and apoptosis. Archives of Biochemistry and Biophysics, 740, 109594.
Sun, Y., Ma, Y., Shi, L., Liu, T., Dong, Y., & Jin, Q. (2025). The Impact and Molecular Mechanisms of Exercise in Cancer Therapy. Current Issues in Molecular Biology, 47(5), 374.
Tobias, G. C., Gomes, J. L., Fernandes, L. G., Voltarelli, V. A., de Almeida, N. R., Jannig, P. R., ... & Brum, P. C. (2023). Aerobic exercise training mitigates tumor growth and cancer-induced splenomegaly through modulation of non-platelet platelet factor 4 expression. Scientific Reports, 13(1), 21970.
Veras, A. S. C., Correia, R. R., Batista, V. R. G., de Almeida Tavares, M. E., Rubira, R. J. G., Fiais, G. A., ... & Teixeira, G. R. (2023). Aerobic physical exercise modifies the prostate tumoral environment. Life Sciences, 332, 122097.
Verma, S., Singh, A., & Mishra, A. (2013). Gallic acid: Molecular rival of cancer. Environmental toxicology and pharmacology, 35(3), 473-485.
Webber, M., Li, M., Cai, C., & Zou, K. (2024). Effects of Exercise Training on Prostate Cancer: Current Evidence and Potential Molecular Mechanisms. Advanced Exercise and Health Science.
Yu, L., Tian, D., Su, Z., Zhang, L., Guo, S., Zhu, W., ... & Zhang, N. (2025). Gallic acid alleviates exercise-induced muscle damage by inhibiting mitochondrial oxidative stress and ferroptosis. Journal of Translational Medicine, 23(1), 30.
Zhu, L., Gu, P., & Shen, H. (2019). Gallic acid improved inflammation via NF-κB pathway in TNBS-induced ulcerative colitis. International immunopharmacology, 67, 129-137.
