High intensity interval training and Eryngium billardieri extract consumption on MMP-2 gene expression of visceral fat in rats with metabolic syndrome
الموضوعات :
Mostafa Haji Gholami
1
(Master of Exercise Physiology, Sports Nutrition.)
Shahin Riyahi Malayeri
2
(Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.)
Asal ziloubaf
3
(Master of Exercise Physiology, Sports Nutrition.)
Hedyeh Hassanpour
4
(Master of Exercise Physiology, Sports Nutrition.)
الکلمات المفتاحية: High intensity interval training, MMP-2, Eryngium billardieri extract, Glucose, Insulin.,
ملخص المقالة :
Background: Metabolic syndrome includes a set of metabolic disorders that expose the patient to cardiovascular disease and diabetes. The aim of the present study was to evaluate the effect of High intensity interval training and Eryngium billardieri extract consumption on the expression of visceral fat matrix metalloproteinase-2 gene and insulin resistance in rats with metabolic syndrome. Methods: In this experimental study, 32 male Sprague-Dawley rats weighing 200-250 g were randomly divided into 4 groups: 1- Healthy group 2- Metabolic syndrome 3- Metabolic syndrome + Eryngium billardieri 4- Metabolic syndrome + Eryngium billardieri + HIIT. The training protocol was performed on a rodent treadmill for 8 weeks and 5 sessions per week and 125 mg of Eryngium billardieri extract per kilogram of body weight was given by gastric gavage 5 days a week for 8 weeks. At the end of the eighth week, sample of visceral adipose tissue was taken to examine the expression of MMP-2 gene. Animal blood was collected from the heart. ANOVA were used for data analysis using SPSS 23 software at a significant level (p <0.05). Tukey significance test was used to compare significant differences. Results: Eight weeks of intense intermittent exercise with consumption of Eryngium billardieri extract caused a significant decrease in MMP-2 gene levels and glucose and insulin levels in comparison with the metabolic syndrome group (P <0.05). Conclusion: According to the results of the study, it seems that High intensity interval training with the use of Eryngium billardieri extract helps to improve insulin resistance and reduce MMP2.
1- Shojaei S, Daneshpour M A, Halalkhor S, Azizi F, Hedayati M. Genetic Association Between Metabolic Syndrome and Apolipoproteins. Iranian Journal of Endocrinology and Metabolism. 2011; 13 (2) :209-220. URL: http://ijem.sbmu.ac.ir/article-1-598-en.html.
2- Alberti KG, Zimmet PZ. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med. 1998 Jul;15(7):539-53. doi: 10.1002/(SICI)1096-9136(199807)15:7<539: AID- DIA668>3.0.CO;2-S. PMID: 9686693.
3- Zabetian A, Hadaegh F, Azizi F. Prevalence of metabolic syndrome in Iranian adult population, concordance between the IDF with the ATPIII and the WHO definitions. Diabetes Res Clin Pract. 2007 Aug;77(2):251-7. doi: 10.1016/j.diabres.2006.12.001. Epub 2007 Jan 16. PMID: 17234299.
4- Medina G, Vera-Lastra O, Peralta-Amaro AL, Jiménez-Arellano MP, Saavedra MA,Cruz-Domínguez MP, Jara LJ. Metabolic syndrome, autoimmunity and rheumatic diseases. Pharmacol Res. 2018 Jul; 133:277-288. doi: 10.1016/j.phrs.2018.01.009. `Epub 2018 Jan 31. PMID: 29382608.
5- Cornier MA, Dabelea D, Hernandez TL, Lindstrom RC, Steig AJ, Stob NR, Van Pelt RE, Wang H, Eckel RH. The metabolic syndrome. Endocr Rev. 2008 Dec;29(7):777-822. doi: 10.1210/er.2008-0024. Epub 2008 Oct 29. PMID: 18971485; PMCID: PMC5393149.
6- Kastorini CM, Milionis HJ, Esposito K, Giugliano D, Goudevenos JA, Panagiotakos DB. The effect of Mediterranean diet on metabolic syndrome and its components: a meta-analysis of 50 studies and 534,906 individuals. J Am Coll Cardiol. 2011 Mar 15;57(11):1299-313. doi: 10.1016/j.jacc.2010.09.073. PMID: 21392646.G.
7- Garvey WT. Phentermine and topiramate extended-release: a new treatment for obesity and its role in a complications-centric approach to obesity medical management. Expert Opin Drug Saf. 2013 Sep;12(5):741-56. doi: 10.1517/14740338.2013.806481. Epub 2013 Jun 6. PMID: 23738843; PMCID: PMC3786546.
8- MacColl E, Khalil RA. Matrix Metalloproteinases as Regulators of Vein Structure and Function: Implications in Chronic Venous Disease. J Pharmacol Exp Ther. 2015 Dec;355(3):410-28. doi: 10.1124/jpet.115.227330. Epub 2015 Aug 28. PMID: 26319699; PMCID: PMC4658486.
9- Henriet P, Emonard H. Matrix metalloproteinase-2: Not (just) a "hero" of the past. Biochimie. 2019 Nov;166:223-232. doi: 10.1016/j.biochi.2019.07.019. Epub 2019 Jul 27. PMID: 31362036.
10- Fazilaty H, Gardaneh M, Bahrami T, Salmaninejad A, Behnam B. Crosstalk between breast cancer stem cells and metastatic niche: emerging molecular metastasis pathway? Tumour Biol. 2013 Aug;34(4):2019-30. doi: 10.1007/s13277-013-0831-y. Epub 2013 May 19. PMID: 23686802.
11- Rashid-lamir A, Fazolahzade-Mousavi R, Mohammad Rahimi G. Effect of Eight Weeks of Aerobic Exercise on the Plasma Levels of Matrix Metalloproteinase in Life Guards: A Pilot Study. Health Educ Health Promot. 2019; 7 (2) :59-63. URL: http://hehp.modares.ac.ir/article-5-15260-en.html.
12- Berg G, Miksztowicz V. Metalloproteinases in the pathogenesis and progression of metabolic syndrome: potential targets for improved outcomes. Metalloproteinases in Medicine. 2015. Volume 2015:2 Pages 51—59. DOI https://doi.org/10.2147/MNM.S88993.
13- Olejarz W, Łacheta D, Kubiak-Tomaszewska G. Matrix Metalloproteinases as Biomarkers of Atherosclerotic Plaque Instability. Int J Mol Sci. 2020 May 31;21(11):3946. doi: 10.3390/ijms21113946. PMID: 32486345; PMCID: PMC7313469.
14- Daneshzadeh M, Abbaspour H, Amjad L, Mohammadi Nafchi A. An investigation on phytochemical, antioxidant and antibacterial properties of extract from Eryngium billardieri billardieri F. Delaroche. Journal of Food Measurement and Characterization. 2019, (Persian).
15- Motallebi Riekandeh S, Mazandarani M, Ebrahimzadeh MA, Zargari M. Antioxidant activities of Eryngium billardieri caucasicum inflorescence. Eur Rev Med Pharmacol Sci. 2016 Mar;20(5):946-9. PMID: 27010155.
16- Paun G, Neagu E, Moroeanu V, Albu C, Savin S, Lucian Radu G. Chemical and Bioactivity Evaluation of Eryngium billardieri planum and Cnicus benedictus Polyphenolic-Rich Extracts. Biomed Res Int. 2019 Mar 12; 2019:3692605. doi: 10.1155/2019/3692605. PMID: 30993111; PMCID: PMC6434295.
17- Castro-Torres IG, De la O-Arciniega M, Naranjo-Rodríguez EB, Castro-Torres VA, Domínguez-Ortíz MÁ, Martínez-Vázquez M. The Hypocholesterolemic Effects of Eryngium billardieri carlinae F. Delaroche Are Mediated by the Involvement of the Intestinal Transporters ABCG5 and ABCG8. Evid Based Complement Alternat Med. 2017; 2017:3176232. doi: 10.1155/2017/3176232. Epub 2017 Dec 14. PMID: 29387127; PMCID: PMC5745665.
18- Roshanravan N, Asgharian P, Dariushnejad H, Mesri Alamdari N, Mansoori B, Mohammadi A, Alipour Sh, Barati M, Ghavami A, Ghorbanzadeh V, Aamazadeh F, Ostadrahimi A. Eryngium billardieri Billardieri Induces Apoptosis via Bax Gene Expression in Pancreatic Cancer Cells Adv Pharm. Bull. 2018. 8, 667–674, (Persian).
19- Tondpa Khaghani B, Dehkhoda M R, Amani Shalamzari S. Improvement of Aerobic Power and Health Status in Overweight Patients with Non-Alcoholic Fatty Liver Disease with High Intensity Interval Training. payavard. 2019; 13 (1) :71-80
URL: http://payavard.tums.ac.ir/article-1-6700-en.html.
20- Bagheri M, Azamian Nazari A, Bani Talebi E, Nasr Esfahani M. A Comparison Between the Effects of Eight Weeks of High Intensity Interval Training and Low Intensity Endurance Training on Expression of Hig2 and Intrahepatic Lipid Content in Rats with Non-Alcoholic Fatty Liver Diseases. Armaghane danesh. 2019; 23 (6) :666-682
URL: http://armaghanj.yums.ac.ir/article-1-2215-en.html.
21- LaForgia J, Withers RT, Gore CJ. Effects of exercise intensity and duration on the excess post-exercise oxygen consumption. J Sports Sci. 2006 Dec;24(12):1247-64. doi: 10.1080/02640410600552064. PMID: 17101527.
22- Lee. M. O. Determination of the surface area of the white rat with its application to the expression of metabolicresults. American Journal of Physiology-Legacy Content 1929; 89(1): 24-33, https://doi.org/10.1152/ajplegacy.1929.89.1.24
23- Hedayati S, Riyahi Malayeri S, Hoseini M. The Effect of Eight Weeks of High and Moderate Intensity Interval Training Along with Aloe Vera Consumption on Serum Levels of Chemerin, Glucose and Insulin in Streptozotocin-induced Diabetic Rats: An Experimental Study. JRUMS. 2018; 17 (9) :801-814. URL: http://journal.rums.ac.ir/article-1-4209-en.html.
24- Machado MV, Vieira AB, da Conceição FG, Nascimento AR, da Nóbrega ACL, Tibirica E. Exercise training dose differentially alters muscle and heart capillary density and metabolic functions in an obese rat with metabolic syndrome. Exp Physiol. 2017 Dec 1;102(12):1716-1728. doi: 10.1113/EP086416. Epub 2017 Oct 26. PMID: 28921743.
25- Erman H, Gelisgen R, Cengiz M, Tabak O, Erdenen F, Uzun H. The association of vascular endothelial growth factor, metalloproteinases and their tissue inhibitors with cardiovascular risk factors in the metabolic syndrome. Eur Rev Med Pharmacol Sci. 2016;20(6):1015-22. PMID: 27049251.
26- Farzanegi P, Akbari A, Azarbayjani M A. Effect of Portulaca oleracea Seeds on the Levels of Matrix Metalloproteinase 2, 9 and Tissue Inhibitor Matrix Metalloproteinase 1 in Patients with Type 2 Diabetes. mjms. 2013; 16 (2) :65-73.URL: http://mjms.modares.ac.ir/article-30-6818-en.html.
27- Atashak S, Batourak K, Azizbeigi K. The effect of moderate-intensity aerobic exercise training on metabolic syndrome factors and acylated ghrelin in middle- age women. RJMS. 2017; 24 (159): 10-20.URL: http://rjms.iums.ac.ir/article-1-4715-en.html.
28- Habibian M, Khosravi M. THE EFFECT OF 8 WEEKS REGULAR SWIMMING EXERCISE ON THE CARDIAC LEVELS OF MATRIX METTALOPROTEINASE-2 AND TRANSFORMING GROWTH FACTOR-Β1 IN DIABETIC RATS. ijdld. 2016; 15 (2) :67-74URL: http://ijdld.tums.ac.ir/article-1-5342-en.html.
29- Bellafiore M, Battaglia G, Bianco A, Farina F, Palma A, Paoli A. The involvement of MMP-2 and MMP-9 in heart exercise-related angiogenesis. J Transl Med. 2013 Nov 7; 11:283. doi: 10.1186/1479-5876-11-283. PMID: 24195673; PMCID: PMC3827823.
30- Akbari N, peeri M, Azarbayjani M A, Delfan M. Comparison of the effect of 8 weeks of continuous and high intensity interval training on the gene expression of TIMP-2 and MMP-2 in male diabetic rats. RJMS. 2019; 26 (10) :107-116. URL: http://rjms.iums.ac.ir/article-1-5702-en.html.
31- Peña-Montes DJ, Huerta-Cervantes M, Ríos-Silva M, Trujillo X, Huerta M, Noriega-Cisneros R, Salgado-Garciglia R, Saavedra-Molina A. Protective Effect of the Hexanic Extract of Eryngium billardieri carlinae Inflorescences In Vitro, in Yeast, and in Streptozotocin-Induced Diabetic Male Rats. Antioxidants (Basel). 2019 Mar 26;8(3):73. doi: 10.3390/antiox8030073. PMID: 30917540; PMCID: PMC6466845.
32- Zhang Y, Gu M, Wang R, Li M, Li D, Xie Z. Dietary supplement of Yunkang 10 green tea and treadmill exercise ameliorate high fat diet induced metabolic syndrome of C57BL/6 J mice. Nutr Metab (Lond). 2020 Feb 4; 17:14. doi: 10.1186/s12986-020-0433-9. PMID: 32042300; PMCID: PMC7001212.
33- Kolahdouzi, S., Talebi Garakani, E., Hamidian, G., Safarzade, A. The Effects of High-Intensity Intermittent Aerobic Training on Adipose Tissue Angiogenesis in Rats Fed a High Fat Diet. Sport Physiology, 2018; 10(38): 143-162. doi: 10.22089/spj.2017.3896.1528
34- Iaccarino G, Franco D, Sorriento D, Strisciuglio T, Barbato E, Morisco C. Modulation of Insulin Sensitivity by Exercise Training: Implications for Cardiovascular Prevention. J Cardiovasc Transl Res. 2021 Apr;14(2):256-270. doi: 10.1007/s12265-020-10057-w. Epub 2020 Jul 31. PMID: 32737757; PMCID: PMC8043859.
35- Khalegi, S. Abedantanzi, H. Gholami, M. The effect of intermittent aerobic exercise and Eryngium billardieri billardieri extract on adipose tissue MCP-1 expression and insulin resistance in male mice with metabolic syndrome, International Conference on Iranian Islamic Medicine, Tehran - University of Tehran, Lamasu Holding - Permanent Secretariat of the Conference. 2019.
36- Sharifi R, Nazari A. Study of Effective Factors on Insulin Sensitivity and Matrix Metalloproteinase in Women with Rheumatoid Arthritis. J Ardabil Univ Med Sci. 2018; 18 (3) :397-409 URL: http://jarums.arums.ac.ir/article-1-1562-en.html.
