Background: Diabetes is a metabolic disorder recognized as one of the most common diseases in the world. The disease has also increased dramatically in Iran.Today, there are many ways to treat diabetes, one of which is the increase in the level of pancreatic beta cells. The increase in these cells is done in several ways Several studies have demonstrated that the lipasin or betatrophin gene, a liver-expressed peptide hormone, increases the proliferation of beta cells, and that overexpression of this gene can increase the number of beta cells. Materials and Methods: The study was conducted on 16 Wistar rats with a mean weight of 160 ± 10. They were induced by diabetes (seven months of diabetes mellitus was caused in rats). They then were divided into two groups of 6: Control (C) and High-Intensity Interval Training (HIIT). Eight weeks of exercise training was conducted on rats.The qRT-PCR technique was used to investigate changes in lipasin expression. An Independent t-test was used for data analysis, and Pearson correlation was used to determine the correlation between lipasin expression and insulin resistance index (P <0.05). Results: The results showed that expression of lipasin gene in the liver of rats in the training group was significantly higher than the control group rats after 8 weeks of training; Insulin resistance index of plasma, plasma insulin and plasma glucose decreased considerably after eight weeks of HIIT. Between lipasin expression and insulin resistance index in rats with type 2 diabetes in the training group, a consider correlation has been observed. Conclusion: This study showed that an 8-week HIIT training period, with increased lipasin expression, could increase beta cells and also recover type 2 diabetes, which had been destroyed by these cells, and as a result of this increase in Insulin secretion and there is a way to prevent the disease. پرونده مقاله