Effect of resistance training and nanocurcumin supplementation on the expression of FNDC5 and PPARƔ genes in rat muscle tissue
محورهای موضوعی : تغذیه ورزشی
Amir Maleki
1
,
Masoumeh Hosseini
2
1 - MSc in Exercise Physiology, Sport Nutrition
2 - Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran.
کلید واژه: Nanocurcumin, FNDC5, PPARƔ Resistance training,
چکیده مقاله :
Background: Irisin is released from the Fndc5 protein in muscle cell through physical activity and effects on metabolism through browning of white fat. The purpose of this study was to the effect of resistance training and supplementation of nanocurcumin on the expression of genes of FNDC5 and PPARɣ rat muscle tissue. Materials and Methods: In this experimental study, 32 rats were randomly divided into four groups (Control, resistance training, nanocurcumin, resistance training + nanocurcumin). The training groups program included 4 weeks, 3 days a week from climbing on a stepladder. Nanocarcmine (80 mg / kg) was given gavage in complementary groups for four weeks daily. FNDC5 and PPARƔ gene expression were measured using the RT-PCR method. Data were analyzed using one-way ANOVA with a significant level of P≤0.05. Results: The results showed that resistance training and supplementation of Nanocarcmine significantly increased the expression of the gene of FNDC5 and PPARɣ in muscle tissue of rat (P <0.05). Conclusion: It seems resistance training with nanocurcumin supplementation may stimulate secretion of FNDC5 & PPARƔ from muscle, that has a key role in the metabolism of adipose tissue and the conversion of white tissue to brown fat tissue.
Background: Irisin is released from the Fndc5 protein in muscle cell through physical activity and effects on metabolism through browning of white fat. The purpose of this study was to the effect of resistance training and supplementation of nanocurcumin on the expression of genes of FNDC5 and PPARɣ rat muscle tissue. Materials and Methods: In this experimental study, 32 rats were randomly divided into four groups (Control, resistance training, nanocurcumin, resistance training + nanocurcumin). The training groups program included 4 weeks, 3 days a week from climbing on a stepladder. Nanocarcmine (80 mg / kg) was given gavage in complementary groups for four weeks daily. FNDC5 and PPARƔ gene expression were measured using the RT-PCR method. Data were analyzed using one-way ANOVA with a significant level of P≤0.05. Results: The results showed that resistance training and supplementation of Nanocarcmine significantly increased the expression of the gene of FNDC5 and PPARɣ in muscle tissue of rat (P <0.05). Conclusion: It seems resistance training with nanocurcumin supplementation may stimulate secretion of FNDC5 & PPARƔ from muscle, that has a key role in the metabolism of adipose tissue and the conversion of white tissue to brown fat tissue.
1. Hosseini M, Eftekhar B, Riyahi Malayeri S. Effect of Interval Training with Curcumin Consumption on Some Adipokines in Menopausal Obese Rats. JRUMS. 2017; 16 (6) :505-516
URL: http://journal.rums.ac.ir/article-1-3644-en.html
2. Lee P, Linderman JD, Smith S, Brychta RJ, Wang J, Idelson C, et al. Irisin and FGF21 are cold-induced endocrine activators of brown fat function in humans. Cell metabolism. 2014;19(2):302-9. DOI: 10.1016/j.cmet.2013.12.017
3. Aghamohammadi M, Habibi A, Ranjbar R. The effect ofselective aerobic training on serum irisin levels andinsulin resistance index in women with type 2 diabetes. Arak Medical University Journal (AMUJ). 2016;18(04):1-9. [Persian]. URL: http://jams.arakmu.ac.ir/article-1-3802-en.html
4. Panati K, Suneetha Y, Narala V.R. Irisin/FNDC5 – Anupdated review. European Review for Medical and Pharmacological Sciences. 2016;20:689-97.
5. Swick A. Irisin, a novel myokine: potential role in obesity and diabetes. Heart Metab. 2013;61:39-40. https://doi.org/10.1155/2018/7816806
6. Lo KA, Sun L. Turning WAT into BAT: areview on regulators controlling the browninof white adipocytes. Biosci Rep. 2013;33(5):e00065. DOI: 10.1042/BSR20130046
7. Hosseinzadeh M, Rashidalmir A, Hejazi SM. The Effect of Progressive Resistance and Endurance Training on Gastrocnemius Muscle’s FNDC5 Gene Expression in Male Rats. Journal of Sabzevar University of Medical Sciences. 2018;25(5):629-37. [Persian]. http://jsums.medsab.ac.ir
8. Han Y. Effects of aerobic exercise on the expressions of skeletal muscle fiber type-related genes and FNDC-5 in high-fat diet-induced obese rats. Interl J Applied Sport Sci. 2017;29(1):42-53. DOI:10.24985/ijass.2017.29.1.42
9. Hashemi-Taklimi M, , Shabani M , Shadmehri S, Sherafati-Moghadam M, Fathalipour M. The effect of 4 weeks High-Intensity Interval Training (HIIT) on the content of PPARγ and PRDM16 in adipose tissue of diabetic obese male rats.Feyz. 2019;23(4):389-97.[ Persian]. URL: http://feyz.kaums.ac.ir/article-1-3791-en.html
10. Hamidnia L, Nematy M, Salari R, Taghipour A, Motavasselian M. Comparing the efficacy of therapeutic packages in Persian Medicine with Classical Medicine in overweight patients: a randomized clinical trial. Electronic physician. 2018;10(6):68-92. [Persian]. DOI: 10.19082/6982
11. Zhang DW, Fu M, Gao SH and Liu JL. Curcumin and diabetes: a systematic review. Evid. Based Complement. Alternat Med. 2013;636053. DOI: 10.1155/2013/636053
12. Aggarwal BB, Gupta S.C, Sung B. Curcumin: an orally bioavailable blocker of TNF and otherpro‐inflammatory biomarkers. Br J Pharmacol. 2013;169(8):1672-92. DOI: 10.1111/bph.12131
13.Lone J, Choi J, Kim S, Yun W. Curcumin induces brown fat-like phenotype in 3T3-L1 and primary white adipocytes. J Nutr Biochem. 2016;27:193-202. DOI: 10.1016/j.jnutbio.2015.09.006
14. Hannoush RN. Synthetic protein lipidation. Current Opinion in Chemical Biology. 2015;28:39-46. https://doi.org/10.1016/j.cbpa.2015.05.025
15. Vijayakurup V, Shankar G M, Retnakumari AP, Nandan CD, Somaraj J, et al. Chitosan Encapsulation Enhances the Bioavailability and Tissue Retention of Curcumin and Improves its Efficacy in Preventing B[a]P-induced Lung Carcinogenesis. Cancer Prevention Research. 2019;12(4):225-36. DOI: 10.1158/1940-6207.
16. Lu Y, Bhat M , Shen SW, Shen ZH, Xu M, Yang CJ, et al. Swimming exercise increases serum irisin level and reduces body fat mass in high-fat-diet fed Wistar rats. Lipids in health and disease . 2016;15(1):93. DOI: 10.1186/s12944-016-0263-y
17. Pang M, Yang J , Rao J, Wang H, Zhang J, Wang S, et al. Time-dependent changes in increased levels of plasma irisin and muscle PGC-1α and FNDC5 after exercise in mice. The Tohoku journal of experimental medicine. 2018;244(2):93-103. DOI: 10.1620/tjem.244.93
18. Khalafi M, Ravasi AA, Soori R, Moradi M, Soleimani M. The acute effects of high intensity interval training (HIIIT)on PGC-1α and FNDC5 genes expression in diabetic rats. Sport biological sciences. 2021;12(3):249-61. [Persian]. DOI: 10.22059/jsb.2020.119643.902
19. Ghaderi M, Mohebbi H, Soltani B. The Effect of 14 Weeks of Endurance Training with Two different Intensity on Serum Irisin Level, Gene Expression of Skeletal Muscle PGC1-α and FNDC5 and Subcutaneous Adipose Tissue UCP1 in Obese Rats. Med J Tabriz Uni Med Sciences Health Services. 2019;41(1):72-81. [Persian]. DOI:10.34172/mj.2019.009
20. Pekkala S, Wiklund PK, Hulmi JJ, Ahtiainen JP, Horttanainen M, Pöllänen E, et al. Are skeletal muscle FNDC5 gene expression and irisin release regulated by exercise and related to health. The Journal of physiology. 2013;591(21):5393-400. DOI: 10.1113/jphysiol.2013.263707
21. Reisi J, Rajabi H, Ghaedi K, Marandi M, samani Z, kazemi nasab F. Effect of eight weeks’resistance training on plasma irisin protein level and muscle FNDC5 and adipose tissue UCP1 genes expression in male rats. Sport physiology.2015;28:117-30. [Persian]. DOI: 20.1001.1.2322164.1394.7.28.8.4
22. Wang S, Wang X , Ye Z, Xu C, Zhang M, Ruan B, and et al. Curcumin promotes browning of white adipose tissue in a norepinephrine-dependent way. Biochem Biophys Res Commun. 2015;466(2):247-53. DOI: 10.1016/j.bbrc.2015.09.018
23. Ray Hamidie R, Yamada T, Ishizawa R, Saito Y, Masuda K. Curcumin treatment enhances the effect of exercise on mitochondrial biogenesis in skeletal muscle by increasing cAMP levels. Metabolism. 2015;64(10):1334-134738. DOI:10.1016/j.metabol.2015.07.010
24. Zou T, Li M Bo W, Zirui W, Yue L, Jinming Y. Curcumin improves insulin sensitivity and increases energy expenditure in high-fat-diet–induced obese mice associated with activation of FNDC5/irisin. Basic nutritional investigation. 2021;90. DOI:10.1016/j.nut.2021.111263
25. Shabani M, Daryanoosh F, Salesi M, Kooshki Jahromi M, Fallahi AA. Effect ofcontinuous training on the level of PPAR-γ and PRDM16 proteins in adipose tissue in overweight diabetes rats. J Qazvin Univ Med Sci. 2018;22(3):4-12. [Farsi]. URL: http://journal.qums.ac.ir/article-1-2627-en.html
26. Turgut M, Cinar V, Pala R, Tuzcu M, Orhan C, Telceken H, et al. Biotinand chromium histidinate improve glucose metabolism and proteins expression levels of IRS-1, PPAR-γ, and NF-κB in exercise-trained rats. J Int Soc Sports Nutr. 2018;15(1):45. DOI: 10.1186/s12970-018-0249-4
27. Adedapo AA, Asunloye OO, Adeoye BO. Peroxisome proliferator-activated receptor-review. International Journal of Biochemistry Research & Review. 2018;21(2):1-19. DOI: 10.9734/IJBCRR/2018/40221
28. Kim J, Lee Y, Kim JC, Ko YH, Yoon CS, Yi H K. Effect of exercise training of diffrentint intensities on anti-inflamatory reaction in sportozotocion-induced diabetic rat. Journal of Biol Sport. 2013;31(1):73-9. DOI: 10.5604/20831862.1093775
29. feizolahi F, Azarbayjani MA , Nasehi M, Peeri M. Combination of Curcumin and Swimming Training Enhances Mitochondrial Biogenesis in the Hippocampal Tissue After Binge Ethanol Drinking in Male Rats. Iranian J Nutr Sci Food Technol. 2018;13(3):1-8. [Persian]. URL: http://nsft.sbmu.ac.ir/article-1-2624-en.html
30. Hemati Farsani Z, Banitalebi E, Faramarzi M, Bigham-Sadegh A. The Effect of Different Intensities Endurance and Resistance Training on Expression Mira and Two Transcription Factors of Osteogenic and Adipogenic, Runx2 and Pparγ on Bone Marrow in Old Male Wistar Rats. Sport Physiology. 2019;42:61-78. [Persian]. DOI: 10.1007/s11033-019-04695-w
