List of Articles Antibiotic resistance

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  1 - Isolation and Determination of Antibiotic Resistance of Salmonella and Staphylococcus Isolated from Dog and Cat Diets
  Ebrahim  Janmohammadi Firooz Mehdi  Sharifi Soltani Atefeh Bozorgi
  Bacterial contamination and widespread use of antibiotics lead to the emergence of antibiotic-resistant bacteria. The aim of this study was to isolate Salmonella and Staphylococcus from diets used for dog and cat and to determine their antibiotic resistance. 50 samples More

  Bacterial contamination and widespread use of antibiotics lead to the emergence of antibiotic-resistant bacteria. The aim of this study was to isolate Salmonella and Staphylococcus from diets used for dog and cat and to determine their antibiotic resistance. 50 samples of dog and cat dry diets were collected. For isolation of Salmonella, culture in liquid Selenite-F, Bismuth sulfite, xylose lysine deoxycholate (XLD) and Salmonella Chromogenic agar were used and for the detection of Staphylococcus, Baird-Parker and mannitol-salt agar. Antibiogram testing was performed by disk diffusion method. Data were analyzed using SPSS 16 software, chi-square test and independent t-test (p≤0.05). Salmonella and Staphylococcus infections were 72% and 20% in dog food and 26% and 80% in cat food, respectively. 100% of the dry bulk feed of cats infected with Salmonella and Staphylococcus and 100% of the samples of dry bulk feed of dogs were infected with Salmonella. 100% of Salmonella strains were resistant to all antibiotics. There was a significant relationship between the level of bacterial contamination and the type of diet (p≤0.05). The presence of bacteria along with antibiotic resistance was identified in this study. Hygiene principles and microbial evaluations are necessary to control and prevent food contamination with bacteria. Manuscript profile
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  2 - Investigating and determining the antibiotic resistance pattern of Acinetobacter baumannii isolated from patients hospitalized in Tehran hospitals by PCR
  Parisa  Majdianfar Setareh Haghighat Farshad Hashemian Bahareh Nowruzi
  Aim and Background: Acinetobacter baumannii is an opportunistic bacterial pathogen responsible for a wide range of hospital-acquired infections. These bacteria take a variety of factors for resistance to different antibiotics, including resistance to β-lactams, aminogly More

  Aim and Background: Acinetobacter baumannii is an opportunistic bacterial pathogen responsible for a wide range of hospital-acquired infections. These bacteria take a variety of factors for resistance to different antibiotics, including resistance to β-lactams, aminoglycosides, and tetracyclines. The aims of this study were to determine the antimicrobial susceptibility pattern and prevalence of bla IMP4, bla CTX-M, tetA, and aadB genes in A. baumannii strains obtained from Imam Khomeini, Bahman, Bu-Ali, and Momenin hospitals. Material and Methods: In this cross-sectional study, 100 clinical Acinetobacter baumannii isolates were collected from various hospitals in Tehran. After the identification of Acinetobacter baumannii isolates by biochemical tests, the antibiotic susceptibility test (Kirby-Bauer method) was done according to CLSI 2021 advice against 8 antibiotics. Finally, the bla IMP4, bla CTX-M, tetA, and aadB genes were determined among the antibiotic-resistant isolates using PCR. Results: According to results, the disc diffusion results showed resistance rates of 90% for ciprofloxacin, 32% ceftazidime, 25% imipenem, 36% gentamicin, 34% streptomycin, 28% piperacillin, 5% polymyxin B and 63% tetracyclin. All isolates were susceptible to colistin. PCR results for bla IMP4, bla CTX-M, tetA, and aadB genes were detected in 63%, 62%, 76%, and 71% of resistant isolates respectively. Conclusion: This study detected clinical A. baumannii isolates harboring antibiotic resistance genes. Identification of antibiotic resistance patterns in A. baumannii and investigation of molecular epidemiology is critical to controlling the rapid spread of antimicrobial-resistant strains. Manuscript profile