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      • Open Access Article

        1 - Cheese whey recycling, the right approach to prevent environmental damage
        Zahra  Katabehei Moradi Maryam  Haghighi khomami Hamed Kioumarsi hooshang dehghanzadeh saeed tamadoni jahromi
        Today, with increasing in production and diversity expansion of dairy products in factories and production of whey products, this food product has become very important. Whey is a water-soluble part of milk that is obtained by adding acid, heating, and coagulating the c More
        Today, with increasing in production and diversity expansion of dairy products in factories and production of whey products, this food product has become very important. Whey is a water-soluble part of milk that is obtained by adding acid, heating, and coagulating the cheese. This yellow liquid contains a lot of lactose and mineral compounds such as calcium and phosphorus, which is important to achieve in order to use as a food source and prevent environmental pollution. Since the wastewaters of the dairy industry have the highest levels of contamination, if the untreated wastewaters of this industry are discharged, serious environmental problems will be inevitable. On the other hand, a reduction in existing reserves and environmental problems followed by increasing use of fossil fuels has prompted researchers to produce new sources of renewable energy, and it can be said that new biological technologies, such as the use of whey as a way to produce biofuels, reduces the environmental problems of dischrging this kind of wastewaters into nature. Today, using biological technology, these compounds can be used to produce biofuels such as hydrogen, methane, and ethanol. So far, various methods and tests have been performed to produce these fuel sources and in this study, the effective strategies for recycling whey and preventing environmental damages are investigated. Manuscript profile
      • Open Access Article

        2 - Isolation and screening of pectinase producing Aspergillus niger and use of whey to optimize enzyme production
        Mahsa pouya shokoofeh Ghazi Amir Tukmechi
        Aims and Background: Pectinase that can break down pectin in plant cell walls is one of the most important industrial enzymes in the world, which can be isolated from a wide range of microorganisms such as bacteria and fungi. Therefore, considering the importance and ap More
        Aims and Background: Pectinase that can break down pectin in plant cell walls is one of the most important industrial enzymes in the world, which can be isolated from a wide range of microorganisms such as bacteria and fungi. Therefore, considering the importance and applications of this enzyme in food processing, agriculture, the purpose of this research is isolating and screening pectinase-producing fungi from some of rotten fruits and vegetables, as well as optimizing the enzyme production condition. Materials and methods: Isolation and identification of pectinase producing fungi from rotten fruits (peach, plum and apple) and vegetables (onion) were done. Isolation and screening the most capable fungal strains producing pectinase was used by cultivation on specific Pectin Agar medium, staining with lactophenol cotton blue and slide culture method. In order to identify the isolated fungi more accurately, the molecular technique like 18S rRNA sequencing was used. Optimizing the production rate of pectinase enzyme carried out using pectin substrate accompanied by different Lactic Whey and Permeate whey under different temperatures and pH levels, and the activity of enzyme was measured by the standard DNS method. Results: one Aspergillus niger fungus producing pectinase enzyme was isolated from onion. Results for optimization conditions shows that the identified strain, shows an enzyme activity equal to 103/018 (IU/ml) in the presence of permeate whey and pectin substrate, under incubation at 25 C° ,140 rpm, and pH 6.5. While, pectinase production with only pure pectin substrate was lower equal to 92.717 (IU/ml) when incubation at 25 C° , same rpm and pH 7. Conclusions: Potent native fungal strains isolated from our country are suitable candidates for the production of important industrial enzymes such as pectinase. Also using cost effective substrates like Whey can be replaced by expensive pure substrates in production industry for the important enzymes production process. Manuscript profile