List of Articles آنزیم

• Open Access Article
  • Abstract Page
  • Full-Text
  
  1 - Evaluation and comparison of some green methods for synthesis of silver nanoparticles
  sepide hamedi seyed abbas  shojaossadati
  10.29252/.3.5.59
  Nowadays, there is an increasing need to develop high-yield, low cost, nontoxic, and eco-friendly procedures for synthesis of metallic nanoparticles. Therefore, the green synthesis methods become customary for synthesis of nanoparticles. Among metallic nanoparticles, na More

  Nowadays, there is an increasing need to develop high-yield, low cost, nontoxic, and eco-friendly procedures for synthesis of metallic nanoparticles. Therefore, the green synthesis methods become customary for synthesis of nanoparticles. Among metallic nanoparticles, nanosilver has developed because of its therapeutic properties. So in this paper, polysaccharide, tollens and biological green methods were investigated. In polysaccharide and tollens methods, starch and β-D glucose were used as a satabilizer and reducer respectively. In biological method biomass and cell filtrate of the Fusarium oxysporum fungus were used for the synthesis of nanoparticles. SEM images and UV-visible absorbtion spectra of these procedures showed that the polysaccharide method produced smaller silver nanoparticles wih high productivity. The changes of NADH-dependant nitrate reductase enzyme activity was evaluated in growh duration by colorometric Harely method due to importance of this enzyme in extracellular synthesis of silver nanoparticles. Results showed that the changes of dry cell mass impact on the enzyme activity. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  2 - Laboratory diagnosis of liver disease in small animal practice
  Saba Ahmadi Morteza  Hasanabadi Mehrdad Mohri
  Today’s accurate diagnosis of disease requires using of different diagnostic and paraclinical methods. Diagnosis of liver disease was a serious challenge both in medicine and veterinary medicine from the past. Clinical biochemistry is one of the main parts of diagnostic More

  Today’s accurate diagnosis of disease requires using of different diagnostic and paraclinical methods. Diagnosis of liver disease was a serious challenge both in medicine and veterinary medicine from the past. Clinical biochemistry is one of the main parts of diagnostic methods. Liver function is evaluated by measuring the variables such as excreting and metabolic functions and enzymes. Because of large functional reserve of liver, symptoms of liver disease appear after loss of huge number of hepatocytes, therefore using of laboratory methods with high specificity and sensitivity could be helpful. None of existing laboratory methods has all characteristics mentioned above. It seems that using different laboratory methods of liver function beside other diagnostic methods such as sonography, cytology and … could be an appropriate approach for reaching a diagnosis of hepatobiliary disease. Current article reviews the perfect utility of liver function tests for general diagnosis of liver disease. Manuscript profile
• Open Access Article
  • Abstract Page
  • Full-Text
  
  3 - Isolation and screening of pectinase producing Aspergillus niger and use of whey to optimize enzyme production
  Mahsa pouya shokoofeh Ghazi Amir Tukmechi
  Aims and Background: Pectinase that can break down pectin in plant cell walls is one of the most important industrial enzymes in the world, which can be isolated from a wide range of microorganisms such as bacteria and fungi. Therefore, considering the importance and ap More

  Aims and Background: Pectinase that can break down pectin in plant cell walls is one of the most important industrial enzymes in the world, which can be isolated from a wide range of microorganisms such as bacteria and fungi. Therefore, considering the importance and applications of this enzyme in food processing, agriculture, the purpose of this research is isolating and screening pectinase-producing fungi from some of rotten fruits and vegetables, as well as optimizing the enzyme production condition. Materials and methods: Isolation and identification of pectinase producing fungi from rotten fruits (peach, plum and apple) and vegetables (onion) were done. Isolation and screening the most capable fungal strains producing pectinase was used by cultivation on specific Pectin Agar medium, staining with lactophenol cotton blue and slide culture method. In order to identify the isolated fungi more accurately, the molecular technique like 18S rRNA sequencing was used. Optimizing the production rate of pectinase enzyme carried out using pectin substrate accompanied by different Lactic Whey and Permeate whey under different temperatures and pH levels, and the activity of enzyme was measured by the standard DNS method. Results: one Aspergillus niger fungus producing pectinase enzyme was isolated from onion. Results for optimization conditions shows that the identified strain, shows an enzyme activity equal to 103/018 (IU/ml) in the presence of permeate whey and pectin substrate, under incubation at 25 C° ,140 rpm, and pH 6.5. While, pectinase production with only pure pectin substrate was lower equal to 92.717 (IU/ml) when incubation at 25 C° , same rpm and pH 7. Conclusions: Potent native fungal strains isolated from our country are suitable candidates for the production of important industrial enzymes such as pectinase. Also using cost effective substrates like Whey can be replaced by expensive pure substrates in production industry for the important enzymes production process. Manuscript profile