The Effect of Swimming Exercise on Interferon-Gamma Gene Expression in Mice with Multiple Sclerosis Exposed to Air Pollution
Subject Areas : Sport PhysiologyMahmood Reza Beig Agha 1 , khosro Jalali Dehkordi 2 , Baharak Moradi Kelardeh 3 , Farzaneh Taghian 4
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Keywords: Air Pollution, Multiple Sclerosis, Interferon-Gamma, Swimming Exercise, Animal Model.,
Abstract :
Background: Interferon-gamma (IFN-γ) is recognized as a key pro-inflammatory cytokine in the pathogenesis of multiple sclerosis (MS). This study aimed to investigate the effect of swimming exercise on IFN-γ levels in mice with experimental autoimmune encephalomyelitis (EAE) exposed to air pollution.
Materials and Methods: In this study, 24 male C57BL/6 mice with an average weight of 24±2 grams were randomly selected and, after an acclimation period, divided into four groups of six: 1) healthy control, 2) EAE-induced, 3) EAE-induced exposed to air pollution, and 4) EAE-induced exposed to air pollution combined with moderate-intensity swimming exercise. EAE was induced by injection of myelin oligodendrocyte glycoprotein (MOG). The swimming protocol was performed five days a week for six weeks. At the end of the study, serum IFN-γ levels were measured using an ELISA kit. Inter-group comparisons were conducted using one-way ANOVA and Tukey's post-hoc test.
Results: IFN-γ levels in the EAE group exposed to air pollution (76.98 ± 2.40 pg/mL) were significantly higher than those in the EAE group (31.20 ± 1.48 pg/mL) and the healthy control group (15.30 ± 1.21 pg/mL) (P<0.05). IFN-γ levels in the exercise group (43.18 ± 3.46 pg/mL) showed a significant decrease compared to the EAE group exposed to air pollution (P<0.05).
Conclusion: Moderate-intensity swimming exercise can significantly modulate the increased IFN-γ levels resulting from simultaneous exposure to an MS model and air pollution.
1.Reich DS. Lucchinetti CF and Calabresi PA. Multiple Sclerosis. New Engl J Med. 2018 Jan 11;378:169-80.
2. Walton C, King R, Rechtman L, Kaye W, Leray E, Marrie RA, et al. Rising prevalence of multiple sclerosis worldwide: Insights from the Atlas of MS. Multiple Sclerosis Journal. 2020 Dec;26(14):1816-21.
3. Arellano G, et al. Role of IFN-γ in MS. Front Immunol. 2022;13:890229.
4. Naghizadeh, N., Riyahi Malayeri, S., Roozbahani, M., Khademi, A., Shirvani, H. The Effectiveness of Endurance Training and Nano Curcumin Supplementation on the Expression of Mir-21and P53 Genes in Brain Tumor Tissue in an Animal Model of Glioblastoma Multiform. Journal of Nutrition,Fasting and Health, 2024; 12(1): 26-35. doi: 10.22038/jnfh.2023.73964.1455.
5. Balashov KE, et al. CCR5+ and CXCR3+ T cells are increased in multiple sclerosis. J Immunol. 2020;205(4):1026-35.
6. Heydarpour P, et al. Air pollution and MS. Neuroepidemiology. 2014;43(3-4):233-8.
7. Ashtari F, et al. Air pollution and MS in Tehran. Iran J Neurol. 2018;17(4):186-9.
8. Miller DB, et al. Air pollution and neuroinflammation. Toxicol Pathol. 2021;49(3):534-54.
9. Dalgas U, Stenager E. Exercise and MS. Ther Adv Neurol Disord. 2012;5(2):81-95.
10. White LJ, Castellano V. Exercise and brain health in MS. Sports Med. 2008;38(2):91-100.
11. Rietberg MB, et al. Exercise therapy for MS. Cochrane Database Syst Rev. 2005;(1):CD003980.
12. Stromnes IM, Goverman JM. Active induction of experimental autoimmune encephalomyelitis. Nat Protoc. 2006;1(4):1810-9.
13. Ghasemi F, et al. Animal exposure system for air pollution. J Environ Health Sci Eng. 2020;18(2):1125-33.
14. Almeida, P.W.M., et al. Swim training suppresses tumor growth in mice. J. Appl. Physiol. 2009;107:261–265.
15. Moradi L, et al. Swimming exercise in MS mice. J Sports Med Phys Fitness. 2022;62(3):345-52.
16. Dehghan F, et al. Moderate intensity exercise in MS. Cytokine. 2021;138:155369.
17. Radak Z, et al. Exercise and oxidative stress. Ageing Res Rev. 2008;7(1):34-42.
18. Petersen AM, Pedersen BK. Anti-inflammatory effect of exercise. J Appl Physiol. 2005;98(4):1154-62.
19. Scheller J, et al. Pro- and anti-inflammatory properties of IL-6. Biochim Biophys Acta. 2011;1813(5):878-88.
20. Bernardes D, et al. Exercise training attenuates experimental autoimmune encephalomyelitis. Brain Behav Immun. 2018;70:75-84.
21. Rossi S, et al. Aerobic exercise affects gene expression in MS. J Neuroimmunol. 2021;355:577575.
22. Radak Z, et al. Exercise, oxidative stress and hormesis. Ageing Res Rev. 2008;7(1):34-42.
23. Petersen AM, Pedersen BK. The anti-inflammatory effect of exercise. J Appl Physiol. 2005;98(4):1154-62.
24. Scheller J, et al. The pro- and anti-inflammatory properties of interleukin-6. Biochim Biophys Acta. 2011;1813(5):878-88.
25. Pedersen BK, Toft AD. Effects of exercise on lymphocytes and cytokines. Br J Sports Med. 2000;34(4):246-51.
26. Calderón-Garcidueñas L, et al. Air pollution and brain inflammation. Toxicol Pathol. 2021;49(3):534-54.
27. Heydarpour P, et al. Air pollution and multiple sclerosis. Neuroepidemiology. 2014;43(3-4):233-8.
28. Rossi S, et al. Neurotrophic factors and exercise in MS. Front Physiol. 2022;13:902892.
