Isolation and screening of facultative halophilic fungi producing industrial enzymes from Tehran Forest parks
Subject Areas : Microbial enzymesMohaddeseh Larypoor 1 , Ensyeh Kargar faragheh 2 , Monyreh Movahedi 3
1 - Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch
2 - Department of Biotechnology, Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch
3 - Department of Biochemistry, Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch
Keywords: Pectinase, Fungi, forest park, halophil,
Abstract :
Aim and Background: Facultative halophilic saprophytic fungi, can produce high amounts of extracellular enzymes with industrial consumption, due to their ease of cultivation, and endurance of severe conditions. In this study, it has been attempted to Assay of enzymatic activity of facultative halophilic fungi from Tehran Forest Parks. Materials and methods: So sampling of soil and air of forest park of Tehran, saprophytes are been identified by the use of microscopic technique and slide culture. Then, the fungi separated were examined qualitatively regarding enzyme activity, and most strains were produced pectinase, lipase, protease, and amylase enzymes. Penicillium halotolerant and E01, E03, E014 strains produced invertase. And only the E13strain produced cellulase.So the enzyme activity of pectinase (via examining the enzyme produced in the media with pectin from pug) in a strain containing the enzyme. In the end stage, the best of enzyme produced strain are been identification to PCR technique and drawn phylogeny tree. Results: Most separated fungi were Aspergillus sp., Penicillium sp., Fusarium sp., Mucor sp., and Rhizopus sp. from among the fungi separated and identified, 19 isolates were considered for producing extracellular enzymes and were identified using molecular methods, such as Fusarium sublunatum, Aspergillus subramanianii, Penicillium hallotolerans, Mucor circinelloides, and Purpureocillium lilacinum. Cladosporium sp., Penicillium halotolerans strains showed the most activity (with activities of 0.71, and 0.79 U/mL). Conclusion: Cladosporium sp, and Penicillium halotolerans due to being cultivated on inexpensive substrates and easy enzyme extraction from the culture media, can be used for industrial enzyme production.
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