The synergy effect of nanoparticles of magnesium oxide and antibiotics on Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa
Subject Areas : Antibiotic ResistanceElham Siasi 1 , Mehrabian Sedigheh 2 , َAli Rafiei 3
1 - 1. Department of Microbiology, Collage of science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
2 - Department of Microbiology, Faculty of Biological science, Islamic Azad University, Tehran North Branch
3 - Department of Microbiology, Faculty of Biological science, Islamic Azad University, Tehran North Branch
Keywords: Magnesium oxide nanoparticles, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa,
Abstract :
Aim and Background: Prevalence of multidrug resistant bacteria,to be necessary simultaneous use of metal nanoparticles and antibiotics for synergistic antimicrobial effects. In this study was studied the synergy antimicrobial effect of magnesium oxide nanoparticles specific concentrations with antibiotics on the standard strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Materials and Methods: After preparation of nano particles and bacteria was used antibiogram by disk diffusion. The antimicrobial properties of nanoparticles were studied by using MIC (Minimum Inhibition concentration) and MBC (Minimum Bactericidal concentration), blank discs and liquid medium. Synergistic effects for bacteria were detected by combining of specific concentration of nanoparticles with antibiotic disks and solution of antibiotics (well-method). Results: The results of the MIC and MBC and liquid medium were confirmed antimicrobial properties of these nanoparticles. E. coli other than the bacteria was more sensitive to lower concentrations of the nanoparticles. Synergistic effect was showed between different concentrations of nanoparticles with methicillin disk in E. coli but, synergistic effect can be observed for three bacteria in the well-method. Conclusion: The results showed synergy effect was observed in all of the bacteria at low concentrations with antibiotics, so this property can be used to reduce the dosage and number of consuming antibiotics.
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