The effect of eight weeks of High Intensity Interval Training (HIIT) on the expression of lipasin in diabetic rats
Subject Areas : Exercise Training and Diseases
Sepideh Salehi
1
,
Nikoo khosravi
2
1 - Masters of Sciences in Exercise Physiology
2 - Department of Physical Education and Sport Sciences, alzahra university, Tehran, Iran
Keywords: HIIT training, insulin resistance, Lipasin, Type 2 diabetes,
Abstract :
Background: Diabetes is a metabolic disorder recognized as one of the most common diseases in the world. The disease has also increased dramatically in Iran.Today, there are many ways to treat diabetes, one of which is the increase in the level of pancreatic beta cells. The increase in these cells is done in several ways Several studies have demonstrated that the lipasin or betatrophin gene, a liver-expressed peptide hormone, increases the proliferation of beta cells, and that overexpression of this gene can increase the number of beta cells. Materials and Methods: The study was conducted on 16 Wistar rats with a mean weight of 160 ± 10. They were induced by diabetes (seven months of diabetes mellitus was caused in rats). They then were divided into two groups of 6: Control (C) and High-Intensity Interval Training (HIIT). Eight weeks of exercise training was conducted on rats.The qRT-PCR technique was used to investigate changes in lipasin expression. An Independent t-test was used for data analysis, and Pearson correlation was used to determine the correlation between lipasin expression and insulin resistance index (P <0.05). Results: The results showed that expression of lipasin gene in the liver of rats in the training group was significantly higher than the control group rats after 8 weeks of training; Insulin resistance index of plasma, plasma insulin and plasma glucose decreased considerably after eight weeks of HIIT. Between lipasin expression and insulin resistance index in rats with type 2 diabetes in the training group, a consider correlation has been observed. Conclusion: This study showed that an 8-week HIIT training period, with increased lipasin expression, could increase beta cells and also recover type 2 diabetes, which had been destroyed by these cells, and as a result of this increase in Insulin secretion and there is a way to prevent the disease.
1. Espes D, Martinell M, Liljebäck H, Carlsson PO. Betatrophin in Diabetes Mellitus: the Epidemiological Evidence in Humans. Curr Diab Rep. 2015 Dec;15(12):104. doi: 10.1007/s11892-015-0676-4. PMID: 26458375.
2. Chen J, Chen S, Huang P, Meng XL, Clayton S, Shen JS, Grayburn PA. In vivo targeted delivery of ANGPTL8 gene for beta cell regeneration in rats. Diabetologia. 2015 May;58(5):1036-44. doi: 10.1007/s00125-015-3521-z. Epub 2015 Feb 28. PMID: 25720603.
3. Ahnfelt-Ronne J, Madsen OD. Betatrophin. Islets. 2014;6(2): e28686.
4. Levitsky LL, Ardestani G, Rhoads DB. Role of growth factors in control of pancreatic beta cell mass: focus on betatrophin. Curr Opin Pediatr. 2014 Aug;26(4):475-9. doi: 10.1097/MOP.0000000000000110. PMID: 24905104.
5. Mohebbi H, Rohani H, Hassan-nia S, Pirooznia N. The Effect of Obesity and Endurance Training-induced Weight Loss on UCP3 mRNA Expression in C57BL/6 MICE. Iranian Journal of Endocrinology and Metabolism. 2013; 15 (3) :311-321
URL: http://ijem.sbmu.ac.ir/article-1-1502-en.html
6. Cox AR, Lam CJ, Bonnyman CW, Chavez J, Rios JS, Kushner JA. Angiopoietin-like protein 8 (ANGPTL8)/betatrophin overexpression does not increase beta cell proliferation in mice. Diabetologia. 2015 Jul;58(7):1523-31. doi: 10.1007/s00125-015-3590-z. Epub 2015 Apr 28. PMID: 25917759; PMCID: PMC4473078.
7. Gusarova V, Alexa CA, Na E, Stevis PE, Xin Y, Bonner-Weir S, Cohen JC, Hobbs HH, Murphy AJ, Yancopoulos GD, Gromada J. ANGPTL8/betatrophin does not control pancreatic beta cell expansion. Cell. 2014 Oct 23;159(3):691-6. doi: 10.1016/j.cell.2014.09.027. PMID: 25417115; PMCID: PMC4243040.
8. Yi P, Park JS, Melton DA. Betatrophin: a hormone that controls pancreatic β cell proliferation. Cell. 2013 May 9;153(4):747-58. doi: 10.1016/j.cell.2013.04.008. Epub 2013 Apr 25. Retraction in: Cell. 2017 Jan 12;168(1-2):326. PMID: 23623304; PMCID: PMC3756510..
9. Zhu JZ, Yu CH, Li YM. Betatrophin provides a new insight into diabetes treatment and lipid metabolism (Review). Biomed Rep. 2014 Jul;2(4):447-451. doi: 10.3892/br.2014.284. Epub 2014 May 20. PMID: 24944788; PMCID: PMC4051489.
10. Wu S, Gao H, Ma Y, Fu L, Zhang C, Luo X. Characterisation of betatrophin concentrations in childhood and adolescent obesity and insulin resistance. Pediatr Diabetes. 2016 Feb;17(1):53-60. doi: 10.1111/pedi.12233. Epub 2014 Nov 21. PMID: 25413012..
11. Wawrusiewicz-Kurylonek N, Telejko B, Kuzmicki M, Sobota A, Lipinska D, Pliszka J, et al. Increased maternal and cord blood betatrophin in gestational diabetes. PloS one. 2015;10(6):e0131171.
12. Erol O, Ellidağ HY, Ayık H, Özel MK, Derbent AU, Yılmaz N. Evaluation of circulating betatrophin levels in gestational diabetes mellitus. Gynecol Endocrinol. 2015;31(8):652-6. doi: 10.3109/09513590.2015.1056142. Epub 2015 Aug 4. PMID: 26291796..
13. Ebert T, Kralisch S, Wurst U, Lössner U, Kratzsch J, Blüher M, Stumvoll M, Tönjes A, Fasshauer M. Betatrophin levels are increased in women with gestational diabetes mellitus compared to healthy pregnant controls. Eur J Endocrinol. 2015 Jul;173(1):1-7. doi: 10.1530/EJE-14-0815. Epub 2015 Apr 7. PMID: 25850828.
14. Yamada H, Saito T, Aoki A, Asano T, Yoshida M, Ikoma A, Kusaka I, Toyoshima H, Kakei M, Ishikawa SE. Circulating betatrophin is elevated in patients with type 1 and type 2 diabetes. Endocr J. 2015;62(5):417-21. doi: 10.1507/endocrj.EJ14-0525. Epub 2015 Mar 5. PMID: 25753914.
15. Abu-Farha M, Sriraman D, Cherian P, AlKhairi I, Elkum N, Behbehani K, et al. Circulating ANGPTL8/Betatrophin Is Increased in Obesity and Reduced after Exercise Training. PLoS One. 2016;11(1): e0147367.
16. Cahová M, Habart D, Olejár T, Berková Z, Papáčková Z, Daňková H, Lodererova A, Heczková M, Saudek F. Lipasin/betatrophin is differentially expressed in liver and white adipose tissue without association with insulin resistance in Wistar and Goto-Kakizaki rats. Physiol Res. 2017 May 4;66(2):273-281. doi: 10.33549/physiolres.933339. Epub 2016 Dec 16. PMID: 27982676.
17. Guo K, Lu J, Yu H, Zhao F, Pan P, Zhang L, Chen H, Bao Y, Jia W. Serum betatrophin concentrations are significantly increased in overweight but not in obese or type 2 diabetic individuals. Obesity (Silver Spring). 2015 Apr;23(4):793-7. doi: 10.1002/oby.21038. Epub 2015 Mar 16. PMID: 25776943.
