Background: Although the beneficial role of exercise and CBD oil in Myocardial infarction (MI) has been reported, the interactive effect of swimming training (ST) and CBD Oil (CO) consumption is still not well known. Therefore, the purpose of this study was to investigate the effect of eight weeks of CBD oil consumption on Ceramide gene expression in the heart tissue of rats suffering from heart infarction along with swimming exercise. Materials and Methods: In this experimental trial, 25 ovariectomized rats with myocardial infarction were divided into five groups, including (1) Healthy control (HC), (2) Myocardial infarction (MI) (3) Myocardial infarction+ Swimming training (MI+ST), (4) Myocardial infarction + 50 mg/kg of CBD oil consumption (MI+CO 50), and (5) Myocardial infarction + 50 mg/kg of CBD oil consumption+ Swimming training (MI+. +CO 50 + ST). Myocardial ischemia was induced by subcutaneous injection of isoproterenol (50 mg/kg intravenously) in myocardial infarction rats. Groups 3 and 4 received 50 mg/kg CBD as gavage on a daily basis for eight weeks, and groups 2 and 4 performed swimming training five days a week. One-way analysis of variance (ANOVA) with Tukey’s post hoc test was performed to analyze the findings (P < 0.05)).In this study, 25 rats were divided in 5 groups including: (1) healthy control, (2) patient control, (3) endurance training (3 days a week for one month), (4) recipients of mesenchymal stem cells (1 × 106 cells / Kg), and (5) simultaneous recipients of endurance training and mesenchymal stem cells. Alkaline phosphatase gene expression was assessed by RT PCR and the amount of osteopontin synthesis was measured by immunohistochemistry procedure. Results: Ceramide in the MI group had a significant decrease compared to the healthy control group (p=0.01). Compared to the MI group, only the MI group + swimming + CBD supplement showed a significant increase in Ceramide (p=0.01). Conclusion: Eight weeks of CBD oil consumption and swimming training can reduce heart tissue damage by increasing Ceramide expression. تفاصيل المقالة