The Effect of Swimming Exercise on Interferon-Gamma Gene Expression in Mice with Multiple Sclerosis Exposed to Air Pollution
الموضوعات : Sport PhysiologyMahmood Reza Beig Agha 1 , خسرو جلالی دهکردی 2 , Baharak Moradi Kelardeh 3 , Farzaneh Taghian 4
1 - Ph.D. Student, Department of Sport Physiology, Isf.C., Islamic Azad University, Isfahan, Iran
2 -
3 - Assistance Professor, Department of Sport Physiology, Isf.C., Islamic Azad University, Isfahan, Iran
4 - Department of Sport Physiology, Isf.C., Islamic Azad University
الکلمات المفتاحية: Air Pollution, Multiple Sclerosis, Interferon-Gamma, Swimming Exercise, Animal Model.,
ملخص المقالة :
Background: Interferon-gamma (IFN-γ) is recognized as a key pro-inflammatory cytokine in the pathogenesis of multiple sclerosis (MS). This study aimed to investigate the effect of swimming exercise on IFN-γ levels in mice with experimental autoimmune encephalomyelitis (EAE) exposed to air pollution.
Materials and Methods: In this study, 24 male C57BL/6 mice with an average weight of 24±2 grams were randomly selected and, after an acclimation period, divided into four groups of six: 1) healthy control, 2) EAE-induced, 3) EAE-induced exposed to air pollution, and 4) EAE-induced exposed to air pollution combined with moderate-intensity swimming exercise. EAE was induced by injection of myelin oligodendrocyte glycoprotein (MOG). The swimming protocol was performed five days a week for six weeks. At the end of the study, serum IFN-γ levels were measured using an ELISA kit. Inter-group comparisons were conducted using one-way ANOVA and Tukey's post-hoc test.
Results: IFN-γ levels in the EAE group exposed to air pollution (76.98 ± 2.40 pg/mL) were significantly higher than those in the EAE group (31.20 ± 1.48 pg/mL) and the healthy control group (15.30 ± 1.21 pg/mL) (P<0.05). IFN-γ levels in the exercise group (43.18 ± 3.46 pg/mL) showed a significant decrease compared to the EAE group exposed to air pollution (P<0.05).
Conclusion: Moderate-intensity swimming exercise can significantly modulate the increased IFN-γ levels resulting from simultaneous exposure to an MS model and air pollution.
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