The effects of aerobic exercise and gallic acid on prostate cancer-related autophagy pathway genes in rats
الموضوعات : Exercise Training and Diseases
Fatemeh Jamshidirad
1
,
Hassan Matin Homaee
2
,
Mohammad Ali Azarbayjani
3
1 - Department of Exercise physiology, CT.C, Islamic Azad University, Tehran, Iran.
2 - Department of Exercise physiology, CT.C, Islamic Azad University, Tehran, Iran.
3 - Department of Exercise physiology, CT.C, Islamic Azad University, Tehran, Iran.
الکلمات المفتاحية: Aerobic exercise, gallic acid, Beclin-1, ATG5, and LC3,
ملخص المقالة :
Prostate cancer is one of the most common cancers. Regular physical activity and medicinal herbs can prevent their development by activating autophagy. However, the simultaneous effect of aerobic exercise and gallic acid on autophagic genes expression in prostate cancer tissues has not been studied. Accordingly, the present study determined the effect of aerobic exercise and gallic acid on the expression of Beclin-1, ATG5 and LC3 genes in the prostate tissue of male rats. In an experimental study, 60 male Wistar rats were randomly divided into 6 groups including 1-control-healthy group, 2-sham group, 3-control-prostate cancer group, 4-prostate cancer-aerobic exercise group, 5-prostate cancer-gallic acid group and 6-prostate cancer-aerobic exercise-gallic acid group. After prostate cancer was induced by inducing LNCaP and TSP-1-ENSCs cell lines, the subjects underwent aerobic exercise and gallic acid administration for eight weeks. At the end of the eighth week, the rats were sacrificed and their prostate tissue was removed. This was done to measure the expression of Beclin-1, ATG5 and LC3 genes by Real-Time PCR. Expression of BECLIN1 (P=0.019), ATG5 (P=0.001) and LC3 (P=0.001) genes was significantly lower in the control-prostate cancer induction group than in the control-healthy group. The expression of BECLIN1, ATG5 and LC3 genes was significantly higher in the aerobic exercise-prostate cancer group, the gallic acid-prostate cancer group and the aerobic exercise-gallic acid-prostate cancer group than in the control-prostate cancer group. According to the study results, it is concluded that aerobic exercise and gallic acid promote autophagy in prostate cancer tissue. It seems that the combination of these two interventions can be used as an effective strategy for managing and preventing disease progression.
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