The Simultaneous effect of high-intensity interval training (HIIT) and omega-3 supplementation on malondialdehyde (MDA) levels in the serum of obese male rats.
محورهای موضوعی : تغذیه ورزشی
Zohreh Nasirizadeh
1
,
Abbas Fattahi Bafghi
2
1 - Department of Physical Education and Sports Sciences, Yazd Branch, Islamic Azad University, Yazd, Iran
2 - Department of Physical Education and Sports Sciences, Yazd Branch, Islamic Azad University, Yazd, Iran
کلید واژه: High Intensity Interval Training, Omega-3, oxidative stress, obese,
چکیده مقاله :
Background: . the aim of this study is to examine the effect of omega-3 fatty acids on Malondialdehyde (MDA) level in long-term aerobic exercise in the serum of obese rats
Materials and methods: In a semi-experimental and applied study, 40 obese male rats of 6 months with Wistar breed with a weighted average of 332.28 Grams and standard deviation of 9 after 7 days of acquaintance with the laboratory environment were randomly divided into 4 groups of 10 (control, training, supplement and training-supplement). The training-supplementation and training groups underwent aerobic training for 6 weeks and 4 sessions in each week, according to the training protocol. Supplement and training- Supplements Groups also for 6 weeks and 4 meals every week, one hour before the start of training, 400 mg of omega-3 per kilogram of body weight was gavage. 24 hours after the last training session, while fasting for 12 hours, all subjects were anesthetized with ketamine and xylazine solution and blood was taken from the posterior orbital sinus of the rats. The collected blood samples were immediately transferred to the laboratory to measure the variables. In order to analyze the data at the descriptive level, mean and standard deviation tests were used, and at the inferential level, the Shapiro-Wilk test was used to check the normality of the data, and the two-way ANOVA test was used to check the interaction effect of the groups. data analysis It was done using spss software version 26 and at a significance level(P≤0.05).
Result: The statistical results of the study showed that all three variables of intensity interval training, Omega-3 supplementation, and exercise-supplementation caused a significant decrease in serum levels of the enzyme malondialdehyde. (p < 0.05).
Conclusion: Based on the results of the present study, it seems that the interactive effect of endurance training and omega-3 supplementation can be used to reduce oxidative damage caused by obesity in the body.
Background: . the aim of this study is to examine the effect of omega-3 fatty acids on Malondialdehyde (MDA) level in long-term aerobic exercise in the serum of obese rats
Materials and methods: In a semi-experimental and applied study, 40 obese male rats of 6 months with Wistar breed with a weighted average of 332.28 Grams and standard deviation of 9 after 7 days of acquaintance with the laboratory environment were randomly divided into 4 groups of 10 (control, training, supplement and training-supplement). The training-supplementation and training groups underwent aerobic training for 6 weeks and 4 sessions in each week, according to the training protocol. Supplement and training- Supplements Groups also for 6 weeks and 4 meals every week, one hour before the start of training, 400 mg of omega-3 per kilogram of body weight was gavage. 24 hours after the last training session, while fasting for 12 hours, all subjects were anesthetized with ketamine and xylazine solution and blood was taken from the posterior orbital sinus of the rats. The collected blood samples were immediately transferred to the laboratory to measure the variables. In order to analyze the data at the descriptive level, mean and standard deviation tests were used, and at the inferential level, the Shapiro-Wilk test was used to check the normality of the data, and the two-way ANOVA test was used to check the interaction effect of the groups. data analysis It was done using spss software version 26 and at a significance level(P≤0.05).
Result: The statistical results of the study showed that all three variables of intensity interval training, Omega-3 supplementation, and exercise-supplementation caused a significant decrease in serum levels of the enzyme malondialdehyde. (p < 0.05).
Conclusion: Based on the results of the present study, it seems that the interactive effect of endurance training and omega-3 supplementation can be used to reduce oxidative damage caused by obesity in the body.
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