The effect CBD oil consumption on Ceramide gene expression in heart tissue of rats with heart infarction along with swimming training
محورهای موضوعی : فیزیولوژی ورزشی
Mostafa Safian Boldaji
1
,
khosro Jalali Dehkordi
2
,
Farzaneh Taghian
3
1 - Ph.D. Student, Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 - Associate Professor, Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
3 - Associate Professor, Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
کلید واژه: Swimming training, CBD oil, Ceramide, Myocardial infarction,
چکیده مقاله :
Background: Although the beneficial role of exercise and CBD oil in Myocardial infarction (MI) has been reported, the interactive effect of swimming training (ST) and CBD Oil (CO) consumption is still not well known. Therefore, the purpose of this study was to investigate the effect of eight weeks of CBD oil consumption on Ceramide gene expression in the heart tissue of rats suffering from heart infarction along with swimming exercise. Materials and Methods: In this experimental trial, 25 ovariectomized rats with myocardial infarction were divided into five groups, including (1) Healthy control (HC), (2) Myocardial infarction (MI) (3) Myocardial infarction+ Swimming training (MI+ST), (4) Myocardial infarction + 50 mg/kg of CBD oil consumption (MI+CO 50), and (5) Myocardial infarction + 50 mg/kg of CBD oil consumption+ Swimming training (MI+. +CO 50 + ST). Myocardial ischemia was induced by subcutaneous injection of isoproterenol (50 mg/kg intravenously) in myocardial infarction rats. Groups 3 and 4 received 50 mg/kg CBD as gavage on a daily basis for eight weeks, and groups 2 and 4 performed swimming training five days a week. One-way analysis of variance (ANOVA) with Tukey’s post hoc test was performed to analyze the findings (P < 0.05)).In this study, 25 rats were divided in 5 groups including: (1) healthy control, (2) patient control, (3) endurance training (3 days a week for one month), (4) recipients of mesenchymal stem cells (1 × 106 cells / Kg), and (5) simultaneous recipients of endurance training and mesenchymal stem cells. Alkaline phosphatase gene expression was assessed by RT PCR and the amount of osteopontin synthesis was measured by immunohistochemistry procedure. Results: Ceramide in the MI group had a significant decrease compared to the healthy control group (p=0.01). Compared to the MI group, only the MI group + swimming + CBD supplement showed a significant increase in Ceramide (p=0.01). Conclusion: Eight weeks of CBD oil consumption and swimming training can reduce heart tissue damage by increasing Ceramide expression.
Background: Although the beneficial role of exercise and CBD oil in Myocardial infarction (MI) has been reported, the interactive effect of swimming training (ST) and CBD Oil (CO) consumption is still not well known. Therefore, the purpose of this study was to investigate the effect of eight weeks of CBD oil consumption on Ceramide gene expression in the heart tissue of rats suffering from heart infarction along with swimming exercise. Materials and Methods: In this experimental trial, 25 ovariectomized rats with myocardial infarction were divided into five groups, including (1) Healthy control (HC), (2) Myocardial infarction (MI) (3) Myocardial infarction+ Swimming training (MI+ST), (4) Myocardial infarction + 50 mg/kg of CBD oil consumption (MI+CO 50), and (5) Myocardial infarction + 50 mg/kg of CBD oil consumption+ Swimming training (MI+. +CO 50 + ST). Myocardial ischemia was induced by subcutaneous injection of isoproterenol (50 mg/kg intravenously) in myocardial infarction rats. Groups 3 and 4 received 50 mg/kg CBD as gavage on a daily basis for eight weeks, and groups 2 and 4 performed swimming training five days a week. One-way analysis of variance (ANOVA) with Tukey’s post hoc test was performed to analyze the findings (P < 0.05)).In this study, 25 rats were divided in 5 groups including: (1) healthy control, (2) patient control, (3) endurance training (3 days a week for one month), (4) recipients of mesenchymal stem cells (1 × 106 cells / Kg), and (5) simultaneous recipients of endurance training and mesenchymal stem cells. Alkaline phosphatase gene expression was assessed by RT PCR and the amount of osteopontin synthesis was measured by immunohistochemistry procedure. Results: Ceramide in the MI group had a significant decrease compared to the healthy control group (p=0.01). Compared to the MI group, only the MI group + swimming + CBD supplement showed a significant increase in Ceramide (p=0.01). Conclusion: Eight weeks of CBD oil consumption and swimming training can reduce heart tissue damage by increasing Ceramide expression.
1. Feng L, Li B, Xi Y, Cai M, Tian Z. Aerobic exercise and resistance exercise alleviate skeletal muscle atrophy through IGF-1/IGF-1R-PI3K/Akt pathway in mice with myocardial infarction. Am J Physiol Cell Physiol. 2022 Feb 1;322(2):C164-C176. doi: 10.1152/ajpcell.00344.2021. Epub 2021 Dec 1. PMID: 34852207.
2. Riyahi Malayeri S, Kaka Abdullah Shirazi S, Behdari R, mousavi Sadati K. Effect of 8-week Swimming training and garlic intake on serum ICAM and VCAM adhesion molecules in male obese rats. . JSSU 2019; 26 (10) :867-878.URL: http://jssu.ssu.ac.ir/article-1-4695-en.html.
3. Riyahi Malayeri, S., Mirakhorli, M. The Effect of 8 Weeks of Moderate Intensity Interval Training on Omentin Levels and Insulin Resistance Index in Obese Adolescent Girls. Sport Physiology & Management Investigations, 2018; 10(2): 59-68. https://www.sportrc.ir/article_67070.html?lang=en
4. Riyahi Malayeri, S., Saei, M. Changes in Insulin resistance and serum levels of resistin after 10 weeks high intensity interval training in overweight and obese men.. Sport Physiology & Management Investigations, 2019; 10(4): 31-42. https://www.sportrc.ir/article_82662.html?lang=en
5. Ding Linlin, Lu Taotao, Wei Wei, Li Yongxu, Lin Libin, Lin Zhicheng, Xue Xiehua. Mechanisms by which swimming exercise and diet control improve hypothalamic lesions in APOE-/- mice with high-fat diet[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(20): 3136-3142. https://www.cjter.com/EN/10.12307/2023.471
6. Bahramian, A., Mirzaei, B., Rahmani nia, F., Karimzade, F. The Effect of Training Exercise Intensity on Left Ventricular Structure and Function in Rats with Myocardial Infarction. Journal of Sport Biosciences, 2019; 11(3): 315-326. doi: 10.22059/jsb.2019.261967.1295 . https://jsb.ut.ac.ir/article_74344.html?lang=en
7. Levin MC, Andersson L, Borén J. Cardiomyocytes, sphingolipids and cardio myotoxicity. Curr Opin Lipidol. 2023 Aug 1;34(4):180-188. doi: 10.1097/MOL.0000000000000829. PMID: 37431304.
8. Changizi, M., fathi, R., alizade, R., Avandi, S. M. Comparison the Effect of two type resistance training on Metabolism ceramide and insulin sensitivity in overweight and obese young men. Sport Physiology, 2022; 14(54): 171-194. doi: 10.22089/spj.2021.10387.2136.https://spj.ssrc.ac.ir/article_2745_en.html.
9. Duan M, Gao P, Chen SX, Novák P, Yin K, Zhu X. Sphingosine-1-phosphate in mitochondrial function and metabolic diseases. Obes Rev. 2022 Jun;23(6):e13426. doi: 10.1111/obr.13426. Epub 2022 Feb 5. PMID: 35122459.
10. Gruzdeva O, Dyleva Y, Belik E, Uchasova E, Ponasenko A, Ivanov S, Zinets M, Stasev A, Kutikhin A, Markova V, Poddubnyak A, Gorbatovskaya E, Fanaskova E, Barbarash O. Expression of Ceramide-Metabolizing Enzymes in the Heart Adipose Tissue of Cardiovascular Disease Patients. Int J Mol Sci. 2023 May 30;24(11):9494. doi: 10.3390/ijms24119494. PMID: 37298446; PMCID: PMC10254038.
11. Banitalebi E, Gharakhanlou R, Ghatrehsamani K, Parnow AH, Teimori H, Mohammad Amoli M. The effect of resistance training on plasma S1P level and gene expression of S1P1,2,3 receptors in male Wistar rats. Minerva Endocrinol. 2013 Dec;38(4):395-400. PMID: 24285107.
12. de Koning IA, van Bakel BMA, Rotbi H, Van Geuns RM, Cramer GE, Pop GAM, Eijsvogels T, Thijssen DHJ. Association between engagement in exercise training and peak cardiac biomarker concentrations following ST-elevation myocardial infarction. BMJ Open Sport Exerc Med. 2023 Apr 11;9(2):e001488. doi: 10.1136/bmjsem-2022-001488. PMID: 37073175; PMCID: PMC10106052.
13. Lai CC, Tang CY, Fu SK, Tseng WC, Tseng KW. Effects of swimming training on myocardial protection in rats. Biomed Rep. 2022 Mar;16(3):19. doi: 10.3892/br.2022.1502. Epub 2022 Jan 31. PMID: 35251606; PMCID: PMC8850963.
14. Aissaoui H, Boulouiz S, El-Azrak M, Bouchlarhem A, Elouafi N, Bazid Z. Cannabis-induced myocardial infarction in a 27-year-old man: Case report. Ann Med Surg (Lond). 2022 Jun 25;80:104054. doi: 10.1016/j.amsu.2022.104054. PMID: 35855878; PMCID: PMC9287769.
15. Walsh SK, Hepburn CY, Kane KA, Wainwright CL. Acute administration of cannabidiol in vivo suppresses ischaemia-induced cardiac arrhythmias and reduces infarct size when given at reperfusion. Br J Pharmacol. 2010 Jul;160(5):1234-42. doi: 10.1111/j.1476-5381.2010.00755.x. PMID: 20590615; PMCID: PMC2936031.
16. Sultan SR, O'Sullivan SE, England TJ. The effects of acute and sustained cannabidiol dosing for seven days on the haemodynamics in healthy men: A randomised controlled trial. Br J Clin Pharmacol. 2020 Jun;86(6):1125-1138. doi: 10.1111/bcp.14225. Epub 2020 Mar 3. PMID: 32128848; PMCID: PMC7256118.
17. Melo SF, Fernandes T, Baraúna VG, Matos KC, Santos AA, Tucci PJ, Oliveira EM. Expression of MicroRNA-29 and Collagen in Cardiac Muscle after Swimming Training in Myocardial-Infarcted Rats. Cell Physiol Biochem. 2014;33(3):657-69. doi: 10.1159/000358642. Epub 2014 Mar 6. PMID: 24642957.
18. Walsh SK, Hepburn CY, Kane KA, Wainwright CL. Acute administration of cannabidiol in vivo suppresses ischaemia-induced cardiac arrhythmias and reduces infarct size when given at reperfusion. Br J Pharmacol. 2010 Jul;160(5):1234-42. doi: 10.1111/j.1476-5381.2010.00755.x. PMID: 20590615; PMCID: PMC2936031.
19. Park LK, Garr Barry V, Hong J, Heebink J, Sah R, Peterson LR. Links between ceramides and cardiac function. Curr Opin Lipidol. 2022 Feb 1;33(1):47-56. doi: 10.1097/MOL.0000000000000802. PMID: 34889803; PMCID: PMC8702478.
20. Gencer B, Morrow DA, Braunwald E, Goodrich EL, Hilvo M, Kauhanen D, Sabatine MS, Laaksonen R, O'Donoghue ML. Plasma ceramide and phospholipid-based risk score and the risk of cardiovascular death in patients after acute coronary syndrome. Eur J Prev Cardiol. 2022 May 6;29(6):895-902. doi: 10.1093/eurjpc/zwaa143. Erratum in: Eur J Prev Cardiol. 2021 Dec 20;28(14):1619. PMID: 33624052.
21. Hoffman M, Palioura D, Kyriazis ID, Cimini M, Badolia R, Rajan S, Gao E, Nikolaidis N, Schulze PC, Goldberg IJ, Kishore R, Yang VW, Bannister TD, Bialkowska AB, Selzman CH, Drakos SG, Drosatos K. Cardiomyocyte Krüppel-Like Factor 5 Promotes De Novo Ceramide Biosynthesis and Contributes to Eccentric Remodeling in Ischemic Cardiomyopathy. Circulation. 2021 Mar 16;143(11):1139-1156. doi: 10.1161/CIRCULATIONAHA.120.047420. Epub 2021 Jan 12. PMID: 33430631; PMCID: PMC7965352.
22. Danieli-Betto D, Peron S, Germinario E, Zanin M, Sorci G, Franzoso S, Sandonà D, Betto R. Sphingosine 1-phosphate signaling is involved in skeletal muscle regeneration. Am J Physiol Cell Physiol. 2010 Mar;298(3):C550-8. doi: 10.1152/ajpcell.00072.2009. Epub 2009 Dec 30. PMID: 20042733.
23. Mohammadi, S., Rostamkhani, F., Riyahi Malayeri, S. et al. High-intensity interval training with probiotic supplementation decreases gene expression of NF-κβ and CXCL2 in small intestine of rats with steatosis. Sport Sci Health 18, 491–497 (2022). https://doi.org/10.1007/s11332-021-00829-5.
24. Riyahi Malayeri, S., Nikbakht, H, Gaeini (2014). Serum Chemerin Levels and Insulin Resistance Response to High- Intensity Interval Training in Overweight Men. Bulletin of Environment, Pharmacology and Life Sciences, 3(2), pp. 385-389.
25. Hosseini M, Ghasem Zadeh Khorasani N, Divkan B, Riyahi Malayeri S. Interactive Effect of High Intensity Interval Training with Vitamin E Consumption on the Serum Levels of Hsp70 and SOD in Male Wistar Rats. Iranian J Nutr Sci Food Technol 2019; 13 (4) :21-28
URL: http://nsft.sbmu.ac.ir/article-1-2689-en.html
26. soleimanifarsani, M., Gharakhanlou, R., Agha alinejad, H., Banitalebi, E., hemati farsani, Z. The effect of resistance training on plasma and cardiac muscle sphingosine-1-phosphate (S1P) levels of male Wistar rat. Journal of Sports and Biomotor Sciences, 2014; 6(12): 38-44. doi: 10.22034/sbs.2023.371643.0.
27. Changizi, M., fathi, R., alizade, R., Avandi, S. M. Comparison the Effect of two type resistance training on Metabolism ceramide and insulin sensitivity in overweight and obese young men. Sport Physiology, 2022; 14(54): 171-194. doi: 10.22089/spj.2021.10387.2136. https://spj.ssrc.ac.ir/article_2745_en.html
28. Dobrzyń A, Górski J. Ceramides and sphingomyelins in skeletal muscles of the rat: content and composition. Effect of prolonged exercise. Am J Physiol Endocrinol Metab. 2002 Feb;282(2):E277-85. doi: 10.1152/ajpendo.00151.2001. PMID: 11788358.
29. Błachnio-Zabielska A, Baranowski M, Zabielski P, Górski J. Effect of exercise duration on the key pathways of ceramide metabolism in rat skeletal muscles. J Cell Biochem. 2008 Oct 15;105(3):776-84. doi: 10.1002/jcb.21877. PMID: 18680146.
30. Rajesh M, Mukhopadhyay P, Bátkai S, Patel V, Saito K, Matsumoto S, Kashiwaya Y, Horváth B, Mukhopadhyay B, Becker L, Haskó G, Liaudet L, Wink DA, Veves A, Mechoulam R, Pacher P. Cannabidiol attenuates cardiac dysfunction, oxidative stress, fibrosis, and inflammatory and cell death signaling pathways in diabetic cardiomyopathy. J Am Coll Cardiol. 2010 Dec 14;56(25):2115-25. doi: 10.1016/j.jacc.2010.07.033. PMID: 21144973; PMCID: PMC3026637.
31. Walsh SK, Hepburn CY, Kane KA, Wainwright CL. Acute administration of cannabidiol in vivo suppresses ischaemia-induced cardiac arrhythmias and reduces infarct size when given at reperfusion. Br J Pharmacol. 2010 Jul;160(5):1234-42. doi: 10.1111/j.1476-5381.2010.00755.x. PMID: 20590615; PMCID: PMC2936031.
