The effects of high-intensity functional training with spinach-derived thylakoid supplementation on Irisin and Lipocalin-2 in obese males
الموضوعات : Sport Physiology
Leila Vakili
1
,
Seyed Ali Hosseini
2
,
Heshmatollah parsian
3
,
Mohammad Ali Azarbayjani
4
,
Rouhallah Ershadi
5
1 - Department of Physical Education and Sport Science, Ki.C., Islamic Azad University, Kish, Iran
2 - Department of Sport Physiology, Marv.C, Islamic Azad University, Marvdasht, Iran
3 - Department of Physical Education and Sport Science, ShQ.C., Islamic Azad University, Shahr-e Qods, Iran
4 - Department of Physical Education and Sport Sciences, CT.C., Islamic Azad University, Tehran, Iran
5 - Department of Physical Education and sport science, Bu.C., Islamic Azad University, Bushehr, Iran
الکلمات المفتاحية: Functional training, Adipokines, thylakoid, Obese Men,
ملخص المقالة :
Introduction: Obesity is a major cause of mortality in developing countries. Increasing physical activity and improving dietary patterns are effective strategies for its prevention and control. This study examined the effects of a 12-week high-intensity functional training (HIFT) program combined with spinach-derived thylakoid supplementation (T) on circulating lipocalin-2 and irisin levels in obese men.
Methods: Forty-four volunteers (mean age 27.6 ± 8.4 years; mean height 168.4 ± 2.6 cm; mean weight 95.7 ± 3.8 kg; mean BMI 32.6 ± 2.6 kg/m2) were randomly assigned to four equal groups (n = 11 each) including placebo control (C), thylakoid supplement (T), HIFT, and HIFT + thylakoid (HT). After baseline measurements, the HIFT and HT groups completed a 12-week HIFT program based on CrossFit principles (3 sessions/week, total 36 sessions; 60 min/session). Supplement groups consumed 5 g/day T powder; C group received 5 g/day raw corn starch (one sachet, taken 30 minutes before lunch) for the same period. Assessments were performed 48 hours before the intervention and 48 hours after the final training session. Between-group differences were analyzed using analysis of covariance (ANCOVA).
Results: ANCOVA revealed significant between-group effects for lipocalin-2 (P< 0.001) and irisin (P= 0.006). Post hoc pair wise comparisons showed significant increases in irisin and decreases in lipocalin-2 for the T, HIFT and HT groups versus C group (P < 0.001).
Conclusion: Twelve weeks of HIFT and daily T supplementation reduced lipocalin-2 and increased irisin concentrations in obese men, with the greatest improvements observed when training and supplementation were combined.
1. Benjamin EJ, Muntner P, Alonso A, Bittencourt MS, Callaway CW, Carson AP, et al. Heart disease and stroke statistics—2019 update: a report from the American Heart Association. Circulation. 2019;139(10):e56-e528.
2. Ayatollahi S, Ghoreshizadeh Z. Prevalence of obesity and overweight among adults in Iran. Obesity reviews. 2010;11(5):335-7.
3. Schwarzenberg SJ, Sinaiko AR. Obesity and inflammation in children. Paediatric Respiratory Reviews. 2006;7(4):239-46.
4. Naghizadeh, N., Riyahi Malayeri, S., Roozbahani, M., Khademi, A., Shirvani, H. The Effectiveness of Endurance Training and Nano Curcumin Supplementation on the Expression of Mir-21and P53 Genes in Brain Tumor Tissue in an Animal Model of Glioblastoma Multiform. Journal of Nutrition,Fasting and Health, 2024; 12(1): 26-35. doi: 10.22038/jnfh.2023.73964.1455
5. Nakamura K, Fuster JJ, Walsh K. Adipokines: a link between obesity and cardiovascular disease. Journal of cardiology. 2014;63(4):250-9.
6. Lee T, Yun S, Jeong JH, Jung TW. Asprosin impairs insulin secretion in response to glucose and viability through TLR4/JNK-mediated inflammation. Molecular and cellular endocrinology. 2019;486:96-104.
7. Li E, Shan H, Chen L, Long A, Zhang Y, Liu Y, et al. OLFR734 mediates glucose metabolism as a receptor of asprosin. Cell metabolism. 2019;30(2):319-28. e8.
8. Al-Daghri NM, Alokeel RM, Alamro A, Ansari MG, Hussain SD, Amer OE, et al. Serum asprosin levels are associated with obesity and insulin resistance in Arab adults. Journal of King Saud University-Science. 2022;34(1):101690.
9. Wen X, Su B, Gao M, Chen J, Zhou D, You H, et al. Obesity-associated up-regulation of lipocalin 2 protects gastric mucosa cells from apoptotic cell death by reducing endoplasmic reticulum stress. Cell Death & Disease. 2021;12(2):221.
10. Zhang J, Wu Y, Zhang Y, LeRoith D, Bernlohr DA, Chen X. The role of lipocalin 2 in the regulation of inflammation in adipocytes and macrophages. Molecular endocrinology. 2008;22(6):1416-26.
11. Wang Y, Lam KS, Kraegen EW, Sweeney G, Zhang J, Tso AW, et al. Lipocalin-2 is an inflammatory marker closely associated with obesity, insulin resistance, and hyperglycemia in humans. Clinical chemistry. 2007;53(1):34-41.
12. Choi K, Kim T, Yoo H, Lee K, Cho G, Hwang T, et al. Effect of exercise training on A‐FABP, lipocalin‐2 and RBP4 levels in obese women. Clinical endocrinology. 2009;70(4):569-74.
13. Yan Q-W, Yang Q, Mody N, Graham TE, Hsu C-H, Xu Z, et al. The adipokine lipocalin 2 is regulated by obesity and promotes insulin resistance. Diabetes. 2007;56(10):2533-40.
14. Wu G, Li H, Fang Q, Jiang S, Zhang L, Zhang J, et al. Elevated circulating lipocalin-2 levels independently predict incident cardiovascular events in men in a population-based cohort. Arteriosclerosis, thrombosis, and vascular biology. 2014;34(11):2457-64.
15. Chen N, Li Q, Liu J, Jia S. Irisin, an exercise‐induced myokine as a metabolic regulator: an updated narrative review. Diabetes/metabolism research and reviews. 2016;32(1):51-9.
16. Arhire LI, Mihalache L, Covasa M. Irisin: a hope in understanding and managing obesity and metabolic syndrome. Frontiers in endocrinology. 2019;10:524.
17. Lujan HL, DiCarlo SE. Physical activity, by enhancing parasympathetic tone and activating the cholinergic anti-inflammatory pathway, is a therapeutic strategy to restrain chronic inflammation and prevent many chronic diseases. Medical hypotheses. 2013;80(5):548-52.
18. Oliveira JS, Pinheiro MB, Fairhall N, Walsh S, Franks TC, Kwok W, et al. Evidence on physical activity and the prevention of frailty and sarcopenia among older people: a systematic review to inform the World Health Organization physical activity guidelines. Journal of Physical Activity and Health. 2020;17(12):1247-58.
19. Feito Y, Heinrich KM, Butcher SJ, Poston WSC. High-intensity functional training (HIFT): Definition and research implications for improved fitness. Sports. 2018;6(3):76.
20. Heinrich KM, Becker C, Carlisle T, Gilmore K, Hauser J, Frye J, et al. High‐intensity functional training improves functional movement and body composition among cancer survivors: a pilot study. European journal of cancer care. 2015;24(6):812-7.
21. Feito Y, Hoffstetter W, Serafini P, Mangine G. Changes in body composition, bone metabolism, strength, and skill-specific performance resulting from 16-weeks of HIFT. PloS one. 2018;13(6):e0198324.
22. Heinrich KM, Patel PM, O’Neal JL, Heinrich BS. High-intensity compared to moderate-intensity training for exercise initiation, enjoyment, adherence, and intentions: an intervention study. BMC public health. 2014;14(1):1-6.
23. Ghanbari-Niaki A, Saeidi A, Ahmadian M, Gharahcholo L, Naghavi N, Fazelzadeh M, et al. The combination of exercise training and Zataria multiflora supplementation increase serum irisin levels in postmenopausal women. Integrative medicine research. 2018;7(1):44-52.
24. Stenblom E-L, Egecioglu E, Landin-Olsson M, Erlanson-Albertsson C. Consumption of thylakoid-rich spinach extract reduces hunger, increases satiety and reduces cravings for palatable food in overweight women. Appetite. 2015;91:209-19.
25. Albertsson P-Å, Köhnke R, Emek SC, Mei J, Rehfeld JF, Åkerlund H-E, et al. Chloroplast membranes retard fat digestion and induce satiety: effect of biological membranes on pancreatic lipase/co-lipase. Biochemical journal. 2007;401(3):727-33.
26. Montelius C, Erlandsson D, Vitija E, Stenblom E-L, Egecioglu E, Erlanson-Albertsson C. Body weight loss, reduced urge for palatable food and increased release of GLP-1 through daily supplementation with green-plant membranes for three months in overweight women. Appetite. 2014;81:295-304.
27. Erlanson-Albertsson C, Albertsson P-Å. The use of green leaf membranes to promote appetite control, suppress hedonic hunger and loose body weight. Plant foods for human nutrition. 2015;70(3):281-90.
28. Feito Y, Patel P, Sal Redondo A, Heinrich KM. Effects of eight weeks of high intensity functional training on glucose control and body composition among overweight and obese adults. Sports. 2019;7(2):51.
29. Glassman G. A theoretical template for crossfit’s programming. CrossFit Journal. 2003;6:1-5.
30. Tabrizi FPF, Farhangi MA, Vaezi M, Hemmati S. The effects of spinach-derived thylakoid supplementation in combination with calorie restriction on anthropometric parameters and metabolic profiles in obese women with polycystic ovary syndrome: a randomized, double-blind, placebo-controlled clinical trial. Nutrition Journal. 2020;19(1):1-13.
31. Gao K, Su Z, Meng J, Yao Y, Li L, Su Y, et al. Effect of exercise training on some anti-inflammatory adipokines, high sensitivity C-reactive protein, and clinical outcomes in Sedentary adults with metabolic syndrome. Biological Research for Nursing. 2024;26(1):125-38.
32. Birar A, Afroundeh R, Hoseiny A. Comparison of the effect of high-intensity functional training (HIFT) and traditional resistance training on the inflammatory status of young obese men. Journal of Applied Health Studies in Sport Physiology. 2024(Articles in Press).
33. Mosialou I, Shikhel S, Luo N, Petropoulou PI, Panitsas K, Bisikirska B, et al. Lipocalin-2 counteracts metabolic dysregulation in obesity and diabetes. Journal of Experimental Medicine. 2020;217(10):e20191261.
34. Mousapoor Sooran A, Ehsan Bakhsh H, Gholami M, Abed Natanzi H. The effect of moderate-intensity continuous training versus high-intensity interval training on the lipocalin 2 levels in obese women. Journal of Exercise & Organ Cross Talk. 2022;2(4):155-60.
35. Salus M, Tillmann V, Remmel L, Unt E, Mäestu E, Parm Ü, et al. Serum osteocalcin, sclerostin and lipocalin-2 levels in adolescent boys with obesity over a 12-week sprint interval training. Children. 2023;10(5):850.
36. Roca-Rivada A, Castelao C, Senin LL, Landrove MO, Baltar J, Crujeiras AB, et al. FNDC5/irisin is not only a myokine but also an adipokine. PloS one. 2013;8(4):e60563.
37. Jia J, Yu F, Wei W-P, Yang P, Zhang R, Sheng Y, et al. Relationship between circulating irisin levels and overweight/obesity: A meta-analysis. World journal of clinical cases. 2019;7(12):1444.
38. Mazur-Bialy AI. Irisin acts as a regulator of macrophages host defense. Life sciences. 2017;176:21-5.
39. Mazur-Bialy AI, Pocheć E, Zarawski M. Anti-inflammatory properties of irisin, mediator of physical activity, are connected with TLR4/MyD88 signaling pathway activation. International journal of molecular sciences. 2017;18(4):701.
40. Jiao P, Chen Q, Shah S, Du J, Tao B, Tzameli I, et al. Obesity-related upregulation of monocyte chemotactic factors in adipocytes: involvement of nuclear factor-κB and c-Jun NH2-terminal kinase pathways. Diabetes. 2009;58(1):104-15.
41. Dong J, Dong Y, Chen F, Mitch W, Zhang L. Inhibition of myostatin in mice improves insulin sensitivity via irisin-mediated cross talk between muscle and adipose tissues. International journal of obesity. 2016;40(3):434-42.
42. Li H, Wang F, Yang M, Sun J, Zhao Y, Tang D. The effect of irisin as a metabolic regulator and its therapeutic potential for obesity. International Journal of Endocrinology. 2021;2021(1):6572342.
43. Torabi A, Reisi J, Kargarfard M, Mansourian M. Differences in the Impact of Various Types of Exercise on Irisin Levels: A Systematic Review and Meta-Analysis. International Journal of Preventive Medicine. 2024;15:11.
44. Jandova T, Buendia-Romero A, Polanska H, Hola V, Rihova M, Vetrovsky T, et al., editors. Long-term effect of exercise on Irisin blood levels—systematic review and meta-analysis. Healthcare; 2021: MDPI.
45. Pourteymour Fard Tabrizi F, Abbasalizad Farhangi M. A systematic review of the potential effects of thylakoids in the management of obesity and its related issues. Food Reviews International. 2021;37(5):469-90.
46. Foshati S, Ekramzadeh M. Thylakoids: a novel food-derived supplement for obesity–a mini-review. International Journal for Vitamin and Nutrition Research. 2019.
47. Amirinejad A, Heshmati J, Shidfar F. Effects of thylakoid intake on appetite and weight loss: a systematic review. Journal of Diabetes & Metabolic Disorders. 2020;19:565-73.
48. Stenkula KG, Stenblom E-L, Montelius C, Egecioglu E, Erlanson-Albertsson C. Thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice. Nutrition & metabolism. 2017;14:1-8.
49. Pourteymour Fard Tabrizi F, Abbasalizad Farhangi M, Vaezi M, Hemmati S. Changes of body composition and circulating neopterin, omentin‐1, and chemerin in response to thylakoid‐rich spinach extract with a hypocaloric diet in obese women with polycystic ovary syndrome: a randomized controlled trial. Phytotherapy Research. 2021;35(5):2594-606.
