Aerobic training affects the expression of oncogenic genes in prostate tissue in male rats
الموضوعات :
Hossein Manzouri
1
,
Mohammad Ali Azarbayjani
2
,
Hasan Matinhomaee
3
1 - Department of Physical Education and Sport Sciences, CT.C., Islamic Azad University, Tehran, Iran.
2 - Department of Physical Education and Sport Sciences, CT.C., Islamic Azad University, Tehran, Iran.
3 - Department of Physical Education and Sport Sciences, CT.C., Islamic Azad University, Tehran, Iran.
الکلمات المفتاحية: Aerobic training, prostate cancer, MMP2, MMP9,
ملخص المقالة :
Since the effect of aerobic training on MMP-9 and MMP-2 gene expression in prostate tissue under cancer conditions is unknown, the present study was conducted to establish the effect of aerobic training on MMP-9 and MMP-2 gene expression in prostate tissue of male rats. In an experimental study, 30 male Wistar rats were randomly divided into 3 groups: 1-control-healthy group, 2-control-prostate cancer group, and 3-prostate cancer-aerobic training group. After prostate cancer was induced using LNCaP and TSP-1-EnSC cell lines, the subjects underwent aerobic training for eight weeks. After prostate cancer was induced using LNCaP and TSP-1-EnSC cell lines, the subjects underwent aerobic training for eight weeks. At the end of the eighth week, the rats were sacrificed and their prostate tissue was removed. This was done to measure MMP-9 and MMP-2 gene expression by Real Time PCR. The results showed that MMP-9 gene expression (P=0.006) was considerably higher in the control-prostate cancer induction group than in the control-healthy group. MMP-9 gene expression (P=0.009) was significantly lower in the prostate cancer-aerobic training group than in the control prostate cancer group. MMP-2 gene expression (P=0.006) was significantly lower in the prostate cancer-aerobic training group than in the control prostate cancer induction group. The results of the present study show that prostate cancer induction is associated with increased expression of MMP-2 and MMP-9 genes, known as oncogenic genes. Aerobic training reduces the expression of MMP-9 and MMP-2 genes, which indicates that aerobic training in induced prostate cancer can prevent prostate tumor development by inhibiting the expression of oncogenic genes, so it is recommended to use aerobic training in prostate cancer conditions.
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