The effect of eight weeks of crocetin consumption and aerobic exercise on the expression of Myod in the heart tissue of elderly prediabetic rats
الموضوعات :Abolhasan Sharifi Rigi 1 , Khosro Jalali Dehkordi 2 , Mohsen Akbarpour Beni 3 , Farzaneh Taghian 4
1 - Ph.D. Student, Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
2 - Associate Professor, Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
3 - Associate Professor, Department of Physical Education and Sport Sciences, University of Qom, Qom, Iran.
4 - Associate Professor, Department of Physical Education and Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
الکلمات المفتاحية: Aerobic exercise, crocetin, Myod, heart, elderly, pre-diabetes,
ملخص المقالة :
Background: Diabetic cardiomyopathy refers to changes in the heart as a result of impaired glucose homeostasis, leading to ventricular dysfunction and associated with mitochondrial dysfunction. Since sports activity is known to protect the heart, the aim of the present study was to investigate aerobic exercise and the effective substance crocetin on the expression of Myod gene in the heart tissue of an experimental model of elderly prediabetic rats. Materials and Methods: In this experimental trial, 25 elderly female c57bl6 male mice with prediabetes (20 mg/kg peritoneal injection) aged 14-16 weeks and weighing 30-35 grams were divided into 5 healthy control groups, prediabetic, prediabetic + aerobic exercise, pre-diabetic + crocetin, pre-diabetic + aerobic exercise + crocetin were divided. aerobic training was performed for eight weeks, five sessions per week. Mice received crocetin 30 mg/kg/day by intraperitoneal injection. Myod expression was measured by Real Time PCR method. To analyze the data, one-way analysis of variance and Tukey's post hoc test were used (P≥0.05). Results: Myod gene expression was significantly decreased in the prediabetic group compared to the healthy group (P=0.01). Myod gene expression was significantly higher in the pre-diabetes group + aerobic exercise + crocetin supplement and diabetes group + aerobic exercise + crocetin supplement than other groups (P=0.01). Conclusion: It seems that intermittent aerobic exercise and crocetin both alone and simultaneously are effective in improving Myod gene expression in prediabetic heart tissue. Therefore, the use of periodic aerobics and crocetin n is recommended in pre-diabetes conditions of old age
1.Jiang Y. J. Excessive ROS production and enhanced autophagy contribute to myocardial injury induced by branched-chain amino acids: Roles for the AMPK-ULK1 signaling pathway and α7nAChR. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 2020; 1867(1): 1-37.
2. Guo Q, Luo Q, Song G. Control of muscle satellite cell function by specific exercise‐induced cytokines and their applications in muscle maintenance. Journal of Cachexia, Sarcopenia and Muscle. 2024.
3. Espino-Gonzalez E, Dalbram E, Mounier R, Gondin J, Farup J, Jessen N, Treebak JT. Impaired skeletal muscle regeneration in diabetes: From cellular and molecular mechanisms to novel treatments. Cell Metabolism. 2024 Mar 7.
4. Aragno, M., Mastrocola, R., Catalano, M. G.,Brignardello, E., Danni, O., & Boccuzzi, G.(2004). Oxidative stress impairs skeletal musclerepair in diabetic rats. Diabetes, 53(4), 1082-1088.
5. Su Z, Yu M, Huang Y, Klein JD, Bian SS, Huang Y, Hassounah F, Chen X, Gao X, Cai H, Wang XH. Electric Acupuncture Mimics Exercise to Promote Myogenesis, Angiogenesis and Neurogenesis. Journal of Biotechnology and Biomedicine. 2024;7:01-14.
6. Marcotte-Chénard A, Little JP. Towards optimizing exercise prescription for type 2 diabetes: modulating exercise parameters to strategically improve glucose control. Translational Exercise Biomedicine. 2024 Apr 16;1(1):71-88.
7. Safian Boldaji M, Jalali Kh, Taghian F. The effect CBD oil consumption on Ceramide gene expression in heart tissue of rats with heart infarction along with swimming training. Journal of Sports Physiology and Athletic Conditioning. 2023 Sep;9(9):81.
8. Tayebi SM, Nouri AH, Tartibian B, Ahmadabadi S, Basereh A, Jamhiri I. Effects of swimming training in hot and cold temperatures combined with cinnamon supplementation on HbA1C levels, TBC1D1, and TBC1D4 in diabetic rats. Nutrition & Diabetes. 2024 Jan 10;14(1):1.
9. Su X, Yuan C, Wang L, Chen R, Li X, Zhang Y, et al. The beneficial effects of saffron extract on potential oxidative stress in cardiovascular diseases. Oxidative medicine and cellular longevity 2021; 19:1-14.
10. Guo ZL, Li MX, Li XL, Wang P, Wang WG, Du WZ, Yang ZQ, Chen SF, Wu D, Tian XY. Crocetin: a systematic review. Frontiers in Pharmacology. 2022 ; 14;12:745683.
11.Jafari A,Zarghami A,Nikokheslat S,Karimi P. effect of eight-week high-intensity interval training (HIIT) along with caffeine injection on the levels of some myocardial autophagy-related proteins in diabetic ratsI.ranian Journal of Diabetes and Metabolism. 2020 ;10;19(4):183-94.
12.Haghparast Azad M, Niktab I, Dastjerdi S, Abedpoor N, Rahimi G, Safaeinejad Z, et al. The combination of endurance exercise and SGTC (Salvia–Ginseng–Trigonella–Cinnamon) ameliorate mitochondrial markers’ overexpression with sufficient ATP production in the skeletal muscle of mice fed AGEs-rich high-fat diet. Nutrition & metabolism. 2022; 5;19(1):17
13. Zhang J, Wang Y, Dong X, Liu J. Crocetin attenuates inflammation and amyloid-β accumulation in APPsw transgenic mice. Immunity & Ageing. 2018;15(1):1-8.
14. Lou X, Zhang Y, Guo J, Gao L, Ding Y, Zhuo X, Lei Q, Bian J, Lei R, Gong W, Zhang X. What is the impact of ferroptosis on diabetic cardiomyopathy: a systematic review. Heart Failure Reviews. 2024 Jan;29(1):1-1.
15. Khan AR, Alnoud MA, Ali H, Ali I, Ahmad S, ul Hassan SS, Shaikh AL, Hussain T, Khan MU, Khan SU, Khan MS. Beyond the beat: A pioneering investigation into exercise modalities for alleviating diabetic cardiomyopathy and enhancing cardiac health. Current Problems in Cardiology. 2023 Nov 23:102222.
16. Zhang F, Lin JJ, Tian HN, Wang J. Effect of exercise on improving myocardial mitochondrial function in decreasing diabetic cardiomyopathy. Experimental Physiology. 2024 Feb;109(2):190-201.
17. Pirani H, Soltany A, Hossein Rezaei M, Khodabakhshi Fard A, Nikooie R, Khoramipoor K, Chamari K, Khoramipour K. Lactate-induced autophagy activation: unraveling the therapeutic impact of high-intensity interval training on insulin resistance in type 2 diabetic rats. Scientific Reports. 2024 Jan 11;14(1):1108.
18. Mehri K, Hamidian G, Babri S, Farajdokht F, Oskuye ZZ. Exercise and insulin glargine administration in mothers with diabetes during pregnancy ameliorate function of testis in offspring: Consequences on apelin-13 and its receptor. Life Sciences. 2024 Feb 22:122517.
19. Pitt JP, McCarthy OM, Hoeg-Jensen T, Wellman BM, Bracken RM. Factors influencing insulin absorption around exercise in type 1 diabetes. Frontiers in Endocrinology. 2020 Oct 21;11:573275.
20. Majd SK, Gholami M, Bazgir B. PAX7 and MyoD proteins expression in response to eccentric and concentric resistance exercise in active young men. Cell Journal (Yakhteh). 2023 Feb;25(2):135.
21. Liu Z, Fu Z, Xia Y, Ding H. The role of BMAL1 and MyoD in exercise-induced skeletal muscle damage. Chinese Journal of Tissue Engineering Research. 2024 Feb 8;28(4):510.
22. Dalle Carbonare L, Dorelli G, Li Vigni V, Minoia A, Bertacco J, Cheri S, Deiana M, Innamorati G, Cominacini M, Tarperi C, Schena F. Physical activity modulates miRNAs levels and enhances MYOD expression in myoblasts. Stem Cell Reviews and Reports. 2022 Jun;18(5):1865-74.
23. Soltanian, Arab Momeni, Allah Yar. Comparison of the effect of two methods of circuit resistance training and intense performance on satellite cell activating factors (MyoD and Myf-5) in young non-athletic men. Community health. 2023 Jun 22;17(2):39-50
24.Majd SK, Gholami M, Bazgir B. PAX7 and MyoD proteins expression in response to eccentric and concentric resistance exercise in active young men. Cell Journal (Yakhteh). 2023 Feb;25(2):135.
25. Gharib MH, Samani KG, ZarrinÅbadi Z, Mokhtari M, Heydarian E. Effect of resveratrol supplementation on antioxidant parameters, lipids profile and several biochemical indices in type 2 diabetic patients: a double-blind randomized-controlled clinical trial. Iranian Journal of Nutrition Sciences & Food Technology 2018;12(4):33-42.
26. Cerdá-Bernad D, Valero-Cases E, Pastor JJ, Frutos MJ. Saffron bioactives crocin, crocetin and safranal: Effect on oxidative stress and mechanisms of action. Critical Reviews in Food Science and Nutrition 2022 ;21;62(12):3232-49.
