Effect of 8-week simultaneous metabolic resistance training and Chlorogenic acid supplement on the expression level of BMP2, BMP4, BMP6, and BMP7: A randomized open label clinical trial
الموضوعات :
Maryam Takhty
1
,
Farshad Ghazalian
2
,
Shahram Soheili
3
,
khosrow Ebrahim
4
1 - Ph.D. student, Department of Sports Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - نویسنده مقالهAssociate professor, Department of Sports Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Assistant professor, Department of Physical education and sport science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.
4 - Professor, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran.
الکلمات المفتاحية: metabolic resistance training, green coffee, obese and overweight women, Bone/body morphogenic proteins,
ملخص المقالة :
Background: BMP has critical role in development, growth, and differentiation of cell. There is enough evidence regarding the role of BMPs in lipid accumulation and homeostasis The current study aimed to evaluate the simultaneous effect of eight weeks of metabolic resistance training (MRT) and Chlorogenic Acid (CGA) supplementation on expression level of BMP2, BMP4, BMP6, and BMP7 in overweight women. Materials and Methods: We carried-out a randomized clinical trial performed on 40 overweight women in Iran 2020. We randomly assigned the study participants into four groups including combined 8-week course of metabolic resistance training (MRT) training and 400 mg chlorogenic acid (CGA) supplementation, 8-week course of MRT, CGA supplement, and the control group. Intervention included three MRT training sessions per week and the duration of each session was 45 minutes. The training exercise intervention was 10 minutes of warm-up, 30 minutes of metabolic resistance training, and 5 minutes of cool-down. The supplementation arms were also received 400 mg / day CGA extracted from green coffee beans. Expression level of BMP2,4,6, and 7 was the main interested outcome that assessed pre and post intervention. Results: We observed significant decrease in BMP2 level in combined intervention group in compared with the control group (Regression coefficient= -2.7, 95% CI=-5.0, -0.4). Moreover, we observed that combined intervention has decreased BMP4 level and the observed difference was statistically significant (Regression coefficient= -6.2, -1.7, -10.6). No significant effect for MRT and CGA group was reported regarding BMP2, and BMP4. Neither combined nor separate form of CGA and MRT had no significant effect on BMP6 and BMP7 (P-value>0.05). Conclusion: Simultaneous MRT exercises and CGA supplementation prohibited expression levels of BMP2, and BMP4. However, they had no significant effect separately. There was no association between the interventions and expression level of BMP6, and BMP7. Abstract Background and Objective: BMP has critical role in development, growth, and differentiation of cell. There is enough evidence regarding the role of BMPs in lipid accumulation and homeostasis The current study aimed to evaluate the simultaneous effect of eight weeks of metabolic resistance training (MRT) and Chlorogenic Acid (CGA) supplementation on expression level of BMP2, BMP4, BMP6, and BMP7 in overweight women. Material and methods: We carried-out a randomized clinical trial performed on 40 overweight women in Iran 2020. We randomly assigned the study participants into four groups including combined 8-week course of metabolic resistance training (MRT) training and 400 mg chlorogenic acid (CGA) supplementation, 8-week course of MRT, CGA supplement, and the control group. Intervention included three MRT training sessions per week and the duration of each session was 45 minutes. The training exercise intervention was 10 minutes of warm-up, 30 minutes of metabolic resistance training, and 5 minutes of cool-down. The supplementation arms were also received 400 mg / day CGA extracted from green coffee beans. Expression level of BMP2,4,6, and 7 was the main interested outcome that assessed pre and post intervention. Results: We observed significant decrease in BMP2 level in combined intervention group in compared with the control group (Regression coefficient= -2.7, 95% CI=-5.0, -0.4). Moreover, we observed that combined intervention has decreased BMP4 level and the observed difference was statistically significant (Regression coefficient= -6.2, -1.7, -10.6). No significant effect for MRT and CGA group was reported regarding BMP2, and BMP4. Neither combined nor separate form of CGA and MRT had no significant effect on BMP6 and BMP7 (P-value>0.05). Conclusion: Simultaneous MRT exercises and CGA supplementation prohibited expression levels of BMP2, and BMP4. However, they had no significant effect separately. There was no association between the interventions and expression level of BMP6, and BMP7.
1. Riyahi Malayeri, S., Saei, M. Changes in Insulin resistance and serum levels of resistin after 10 weeks high intensity interval training in overweight and obese men. Sport Physiology & Management Investigations, 2019; 10(4): 31-42. http://www.sportrc.ir/article_82662.html?lang=en.
2. Ashtary-Larky D, Kashkooli S, Bagheri R, Lamuchi-Deli N, Alipour M, Mombaini D, Baker JS, Ramezani Ahmadi A, Wong A. The effect of exercise training on serum concentrations of chemerin in patients with metabolic diseases: a systematic review and meta-analysis. Arch Physiol Biochem. 2021 Mar 2:1-10. doi: 10.1080/13813455.2021.1892149. Epub ahead of print. PMID: 33651961.
3. Farazandeh Nia, D., Hosseini, M., Riyahi Malayeri, S., Daneshjoo, A. Effect of Eight Weeks of Swimming Training with Garlic Intake on Serum Levels of IL-10 and TNF-α in Obese Male Rats. Jundishapur Scientific Medical Journal, 2018; 16(6): 665-671. doi: 10.22118/jsmj.2018.57830
4. Riyahi Malayeri S, Kaka Abdullah Shirazi S, Behdari R, mousavi Sadati K. Effect of 8-week Swimming training and garlic intake on serum ICAM and VCAM adhesion molecules in male obese rats. JSSU 2019; 26 (10): 867-878.URL: http://jssu.ssu.ac.ir/article-1-4695-en.html.
5. Gobeske KT, Das S, Bonaguidi MA, Weiss C, Radulovic J, Disterhoft JF, Kessler JA. BMP signaling mediates effects of exercise on hippocampal neurogenesis and cognition in mice. PLoS One. 2009 Oct 20;4(10):e7506. doi: 10.1371/journal.pone.0007506. PMID: 19841742; PMCID: PMC2759555.
6. Wu MY, Hill CS. Tgf-beta superfamily signaling in embryonic development and homeostasis. Dev Cell. 2009 Mar;16(3):329-43. doi: 10.1016/j.devcel.2009.02.012. PMID: 19289080.
7. Clark JF, Ciccarelli EJ, Kayastha P, Ranepura G, Yamamoto KK, Hasan MS, et al. BMP pathway regulation of insulin signaling components promotes lipid storage in Caenorhabditis elegans. PLoS genetics. 2021;17(10):e1009836. https://doi.org/10.1371/journal.pgen.1009836.
8. Whittle AJ, Carobbio S, Martins L, Slawik M, Hondares E, Vázquez MJ, Morgan D, Csikasz RI, Gallego R, Rodriguez-Cuenca S, Dale M, Virtue S, Villarroya F, Cannon B, Rahmouni K, López M, Vidal-Puig A. BMP8B increases brown adipose tissue thermogenesis through both central and peripheral actions. Cell. 2012 May 11;149(4):871-85. doi: 10.1016/j.cell.2012.02.066. PMID: 22579288; PMCID: PMC3383997.
9. Riyahi Malayeri S, Abdolhay S, Behdari R, Hoseini M. The combined effect of resveratrol supplement and endurance training on IL-10 and TNF- α in type 2 diabetic rats. RJMS 2019; 25 (12) :140-149.URL: http://rjms.iums.ac.ir/article-1-5526-en.html.
10. Sadeghi Eshtehardi F, Peeri M, Azarbayjani MA. The effect of Different Intensity Circuit Resistance Training on the Levels of Selected Adipokines (WISP-1, WISP-2, BMP4) in Obese Postmenopausal Women. Razi Journal of Medical Sciences. 2022;28(12):15-27. URL: http://rjms.iums.ac.ir/article-1-6983-en.html.
11. Mao H, Li L, Fan Q, Angelini A, Saha PK, Wu H, Ballantyne CM, Hartig SM, Xie L, Pi X. Loss of bone morphogenetic protein-binding endothelial regulator causes insulin resistance. Nat Commun. 2021 Mar 26;12(1):1927. doi: 10.1038/s41467-021-22130-2. PMID: 33772019; PMCID: PMC7997910.
12. Chattopadhyay T, Singh RR, Gupta S, Surolia A. Bone morphogenetic protein-7 (BMP-7) augments insulin sensitivity in mice with type II diabetes mellitus by potentiating PI3K/AKT pathway. Biofactors. 2017 Mar;43(2):195-209. doi: 10.1002/biof.1334. Epub 2017 Feb 10. PMID: 28186649.
13. Xu X, Li X, Yang G, Li L, Hu W, Zhang L, Liu H, Zheng H, Tan M, Zhu D. Circulating bone morphogenetic protein-9 in relation to metabolic syndrome and insulin resistance. Sci Rep. 2017 Dec 13;7(1):17529. doi: 10.1038/s41598-017-17807-y. PMID: 29235531; PMCID: PMC5727514.
14. Rocha-Rodrigues S, Rodríguez A, Gouveia AM, Gonçalves IO, Becerril S, Ramírez B, Beleza J, Frühbeck G, Ascensão A, Magalhães J. Effects of physical exercise on myokines expression and brown adipose-like phenotype modulation in rats fed a high-fat diet. Life Sci. 2016 Nov 15;165:100-108. doi: 10.1016/j.lfs.2016.09.023. Epub 2016 Sep 28. PMID: 27693382.
15. Takhty M, Ghazalian F, Sohili S, Ebrahim K. Simultaneous Effect of Eight Weeks of Metabolic Resistance Training and CGA Supplementation on Weight Loss and Fat Profile of Overweight Women. RJMS 2022; 29 (7) :23-32.URL: http://rjms.iums.ac.ir/article-1-7593-fa.html.
16. Roshan H, Nikpayam O, Sedaghat M, Sohrab G. Effects of green coffee extract supplementation on anthropometric indices, glycaemic control, blood pressure, lipid profile, insulin resistance and appetite in patients with the metabolic syndrome: a randomised clinical trial. Br J Nutr. 2018 Feb;119(3):250-258. doi: 10.1017/S0007114517003439. Epub 2018 Jan 8. PMID: 29307310.
17. Schreiber, I., Dörpholz, G., Ott, CE. et al. BMPs as new insulin sensitizers: enhanced glucose uptake in mature 3T3-L1 adipocytes via PPARγ and GLUT4 upregulation. Sci Rep 7, 17192 (2017). https://doi.org/10.1038/s41598-017-17595-5
18. Tseng YH, Kokkotou E, Schulz TJ, Huang TL, Winnay JN, Taniguchi CM, Tran TT, Suzuki R, Espinoza DO, Yamamoto Y, Ahrens MJ, Dudley AT, Norris AW, Kulkarni RN, Kahn CR. New role of bone morphogenetic protein 7 in brown adipogenesis and energy expenditure. Nature. 2008 Aug 21;454(7207):1000-4. doi: 10.1038/nature07221. PMID: 18719589; PMCID: PMC2745972.
19. Hoffmann JM, Grünberg JR, Church C, Elias I, Palsdottir V, Jansson JO, Bosch F, Hammarstedt A, Hedjazifar S, Smith U. BMP4 Gene Therapy in Mature Mice Reduces BAT Activation but Protects from Obesity by Browning Subcutaneous Adipose Tissue. Cell Rep. 2017 Aug 1;20(5):1038-1049. doi: 10.1016/j.celrep.2017.07.020. PMID: 28768190.
20. Guiu-Jurado E, Unthan M, Böhler N, Kern M, Landgraf K, Dietrich A, Schleinitz D, Ruschke K, Klöting N, Faßhauer M, Tönjes A, Stumvoll M, Körner A, Kovacs P, Blüher M. Bone morphogenetic protein 2 (BMP2) may contribute to partition of energy storage into visceral and subcutaneous fat depots. Obesity (Silver Spring). 2016 Oct;24(10):2092-100. doi: 10.1002/oby.21571. Epub 2016 Aug 12. PMID: 27515773.
21. Fujita K, Otsuka T, Yamamoto N, Kainuma S, Ohguchi R, Kawabata T, Sakai G, Kuroyanagi G, Matsushima-Nishiwaki R, Kozawa O, Tokuda H. (-)-Epigallocatechin gallate but not chlorogenic acid upregulates osteoprotegerin synthesis through regulation of bone morphogenetic protein-4 in osteoblasts. Exp Ther Med. 2017 Jul;14(1):417-423. doi: 10.3892/etm.2017.4491. Epub 2017 May 22. PMID: 28672948; PMCID: PMC5488595.
22. Majerczak J, Filipowska J, Tylko G, Guzik M, Karasinski J, Piechowicz E, Pyza E, Chlopicki S, Zoladz JA. Impact of long-lasting spontaneous physical activity on bone morphogenetic protein 4 in the heart and tibia in murine model of heart failure. Physiol Rep. 2020 Apr;8(8):e14412. doi: 10.14814/phy2.14412. PMID: 32319199; PMCID: PMC7174143.
23. Keller MP, Choi Y, Wang P, Davis DB, Rabaglia ME, Oler AT, Stapleton DS, Argmann C, Schueler KL, Edwards S, Steinberg HA, Chaibub Neto E, Kleinhanz R, Turner S, Hellerstein MK, Schadt EE, Yandell BS, Kendziorski C, Attie AD. A gene expression network model of type 2 diabetes links cell cycle regulation in islets with diabetes susceptibility. Genome Res. 2008 May;18(5):706-16. doi: 10.1101/gr.074914.107. Epub 2008 Mar 17. PMID: 18347327; PMCID: PMC2336811.
