Effect of 8-week metabolic resistance training and Chlorogenic acid supplement on the expression of UCP1: A randomized clinical trial
محورهای موضوعی : تغذیه ورزشی
Maryam Takhty
1
,
Farshad Ghazalian
2
,
Shahram Soheili
3
,
khosrow Ebrahim
4
1 - Ph.D. student, Department of Sports Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Associate professor, Department of Sports Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Assistant professor, Department of Physical education and sport science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.
4 - Professor, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran.
کلید واژه: metabolic resistance training, green coffee, obese and overweight women, Uncoupling Protein 1. ,
چکیده مقاله :
Background: Obesity and overweight is one of the major public health challenges all around the world. The aim of this study was to evaluate the simultaneous effect of eight weeks of metabolic resistance training (MRT) and Chlorogenic Acid (CGA) supplementation on weight loss, expression level of UCP1in overweight women. Materials and Methods: The present study was a randomized clinical trial performed in 2022 on a sample of overweight women in Iran. Participants in the study were randomly divided into four groups including combined 8-week course of MRT training and CGA supplementation, 8-week course of MRT, CGA supplement, and the control group. Intervention included three training sessions per week and the duration of each session was 45 minutes. The supplementation arms were also received 400 mg / day CGA extracted from green coffee beans. Expression level of Uncoupling Protein 1 (UCP1) was the main interested outcome that assessed pre and post intervention. Results: In the MRT exercise group, UCP1 expression increased by 4.3 units on average over the 8-week intervention. The highest increase was observed in participants who received both CGA supplement and MRT exercise where UCP1 increased from 22.5 (1.2) to 28.0 (3.5) over the study period (P<0.05). No significant increase was observed in CGA supplementation group. Conclusion: MRT exercises with and without CGA supplementation stimulated expression level of UCP1 mRNA.
Background: Obesity and overweight is one of the major public health challenges all around the world. The aim of this study was to evaluate the simultaneous effect of eight weeks of metabolic resistance training (MRT) and Chlorogenic Acid (CGA) supplementation on weight loss, expression level of UCP1in overweight women. Materials and Methods: The present study was a randomized clinical trial performed in 2022 on a sample of overweight women in Iran. Participants in the study were randomly divided into four groups including combined 8-week course of MRT training and CGA supplementation, 8-week course of MRT, CGA supplement, and the control group. Intervention included three training sessions per week and the duration of each session was 45 minutes. The supplementation arms were also received 400 mg / day CGA extracted from green coffee beans. Expression level of Uncoupling Protein 1 (UCP1) was the main interested outcome that assessed pre and post intervention. Results: In the MRT exercise group, UCP1 expression increased by 4.3 units on average over the 8-week intervention. The highest increase was observed in participants who received both CGA supplement and MRT exercise where UCP1 increased from 22.5 (1.2) to 28.0 (3.5) over the study period (P<0.05). No significant increase was observed in CGA supplementation group. Conclusion: MRT exercises with and without CGA supplementation stimulated expression level of UCP1 mRNA.
1. Sarma S, Sockalingam S, Dash S. Obesity as a multisystem disease: Trends in obesity rates and obesity-related complications. Diabetes Obes Metab. 2021 Feb;23 Suppl 1:3-16. doi: 10.1111/dom.14290. PMID: 33621415.
2. WHO. Obesity and overweight. World Health Organization. 2021.
3. Farazandeh Nia, D., Hosseini, M., Riyahi Malayeri, S., Daneshjoo, A. Effect of Eight Weeks of Swimming Training with Garlic Intake on Serum Levels of IL-10 and TNF-α in Obese Male Rats. Jundishapur Scientific Medical Journal, 2018; 16(6): 665-671. doi: 10.22118/jsmj.2018.57830.
4. Haghighi AH, Yaghoubi M. The effect of eight weeks aerobic training and green tea supplementation on body fat percentage and serum lipid profiles in obese and overweight women. Medical journal of mashhad university of medical sciences. 2013;56(4):211-8.
5. Riyahi Malayeri S, Mirakhorli M. The Effect of 8 Weeks of Moderate Intensity Interval Training on Omentin Levels and Insulin Resistance Index in Obese Adolescent Girls. Sport Physiology & Management Investigations. 2018;10(2):59-68. http://www.sportrc.ir/article_67070.html?lang=en.
6. Riyahi Malayeri S, Saei M. Changes in Insulin resistance and serum levels of resistin after 10 weeks high intensity interval training in overweight and obese men. Sport Physiology & Management Investigations. 2019;10(4):31-42. http://www.sportrc.ir/article_82662.html?lang=en.
7. Hosseini M, Naderi S, Mousavi-Sadati, S K, Riyahi S. Effect of High Intensity Interval Training on the Level of Leptin and Liver Enzymes in Obese and Overweight Males. sjimu 2019; 27 (2) :41-50. doi: 10.29252/sjimu.27.2.41
8. Mann S, Beedie C, Jimenez A. Differential effects of aerobic exercise, resistance training and combined exercise modalities on cholesterol and the lipid profile: review, synthesis and recommendations. Sports Med. 2014 Feb;44(2):211-21. doi: 10.1007/s40279-013-0110-5. PMID: 24174305; PMCID: PMC3906547.
9. Ong KW, Hsu A, Tan BK. Anti-diabetic and anti-lipidemic effects of chlorogenic acid are mediated by ampk activation. Biochem Pharmacol. 2013 May 1;85(9):1341-51. doi: 10.1016/j.bcp.2013.02.008. Epub 2013 Feb 14. PMID: 23416115.
10. Onakpoya I, Terry R, Ernst E. The use of green coffee extract as a weight loss supplement: a systematic review and meta-analysis of randomised clinical trials. Gastroenterol Res Pract. 2011;2011:382852. doi: 10.1155/2011/382852. Epub 2010 Aug 31. PMID: 20871849; PMCID: PMC2943088..
11. Ong KW, Hsu A, Tan BK. Anti-diabetic and anti-lipidemic effects of chlorogenic acid are mediated by ampk activation. Biochem Pharmacol. 2013 May 1;85(9):1341-51. doi: 10.1016/j.bcp.2013.02.008. Epub 2013 Feb 14. PMID: 23416115.
12. Yau WW, Yen PM. Thermogenesis in Adipose Tissue Activated by Thyroid Hormone. Int J Mol Sci. 2020 Apr 24;21(8):3020. doi: 10.3390/ijms21083020. PMID: 32344721; PMCID: PMC7215895.
13. Gaudry MJ, Keuper M, Jastroch M. Molecular evolution of thermogenic uncoupling protein 1 and implications for medical intervention of human disease. Mol Aspects Med. 2019 Aug;68:6-17. doi: 10.1016/j.mam.2019.06.006. Epub 2019 Jul 18. PMID: 31238069.
14. Roshan H, Nikpayam O, Sedaghat M, Sohrab G. Effects of green coffee extract supplementation on anthropometric indices, glycaemic control, blood pressure, lipid profile, insulin resistance and appetite in patients with the metabolic syndrome: a randomised clinical trial. Br J Nutr. 2018 Feb;119(3):250-258. doi: 10.1017/S0007114517003439. Epub 2018 Jan 8. PMID: 29307310.
15. Daneshyar S, OmidAli F, Feizipour S A. THE COMBINED EFFECT OF LONG-TERM FEEDING OF HIGH-FAT DIET AND REGULAR AEROBIC TRAINING ON GENE EXPRESSION OF UNCOUPLING PROTEIN 1 (UCP1) IN BROWN ADIPOSE TISSUE AND SARCOLIPIN (SLN) IN SOLEUS MUSCLE OF MICE: AN EXPERIMENTAL STUDY. Studies in Medical Sciences 2021; 32 (4) :290-302
URL: http://umj.umsu.ac.ir/article-1-5341-en.html
16. Xu X, Ying Z, Cai M, Xu Z, Li Y, Jiang SY, Tzan K, Wang A, Parthasarathy S, He G, Rajagopalan S, Sun Q. Exercise ameliorates high-fat diet-induced metabolic and vascular dysfunction, and increases adipocyte progenitor cell population in brown adipose tissue. Am J Physiol Regul Integr Comp Physiol. 2011 May;300(5):R1115-25. doi: 10.1152/ajpregu.00806.2010. Epub 2011 Mar 2. PMID: 21368268; PMCID: PMC3094041.
17. Daneshyar S, Kordi MR, Gaeini A, Kadivar M, Afshari S. The effect of endurance training on gene expression of Uncoupling Protein 1 (UCP-1) in white visceral adipose tissue of retroperitoneal depot of male Wistar rats. Razi Journal of Medical Sciences. 2015;22(136):35-45.
18. Shirkhani S, Marandi SM, Kazeminasab F, Esmaeili M, Ghaedi K, Esfarjani F, Shiralian-Esfahani H, Nasr-Esfahani MH. Comparative studies on the effects of high-fat diet, endurance training and obesity on Ucp1 expression in male C57BL/6 mice. Gene. 2018 Nov 15;676:16-21. doi: 10.1016/j.gene.2018.07.015. Epub 2018 Jul 6. PMID: 30201103.
19. Deng H, Zhang W, Ruan D, Chen D, Xu X, He X, et al. The Combination of Aerobic and Resistance Exercise Induces Weight Loss via the PGC-1α/Irisin/UCP-1 Pathway. Journal of Biomaterials and Tissue Engineering. 2019;9(10):1388-94.
20. Flouris AD, Dinas PC, Valente A, Andrade CMB, Kawashita NH, Sakellariou P. Exercise-induced effects on UCP1 expression in classical brown adipose tissue: a systematic review. Horm Mol Biol Clin Investig. 2017 Jan 13;31(2):/j/hmbci.2017.31.issue-2/hmbci-2016-0048/hmbci-2016-0048.xml. doi: 10.1515/hmbci-2016-0048. PMID: 28085671.
21. Ye X, Li J, Gao Z, Wang D, Wang H, Wu J. Chlorogenic Acid Inhibits Lipid Deposition by Regulating the Enterohepatic FXR-FGF15 Pathway. Biomed Res Int. 2022 Feb 25;2022:4919153. doi: 10.1155/2022/4919153. PMID: 35257010; PMCID: PMC8897747.
22. Wang G, Meyer JG, Cai W, Softic S, Li ME, Verdin E, Newgard C, Schilling B, Kahn CR. Regulation of UCP1 and Mitochondrial Metabolism in Brown Adipose Tissue by Reversible Succinylation. Mol Cell. 2019 May 16;74(4):844-857.e7. doi: 10.1016/j.molcel.2019.03.021. Epub 2019 Apr 15. PMID: 31000437; PMCID: PMC6525068.
23. Huang K, Liang XC, Zhong YL, He WY, Wang Z. 5-Caffeoylquinic acid decreases diet-induced obesity in rats by modulating PPARα and LXRα transcription. J Sci Food Agric. 2015 Jul;95(9):1903-10. doi: 10.1002/jsfa.6896. Epub 2014 Oct 1. PMID: 25186103.
24. Sudhakar M, Sasikumar SJ, Silambanan S, Natarajan D, Ramakrishnan R, Nair AJ, et al. Chlorogenic acid promotes development of brown adipocyte-like phenotype in 3T3-L1 adipocytes. Journal of Functional Foods. 2020;74:104161.
